Protein microarray chip with Ni–Co alloy coated surface

Chang, Yaw-Jen; Chang, Chao‐Yuan

doi:10.1016/j.bios.2009.12.030

Cited by 20 publications

(6 citation statements)

References 28 publications

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“…The Taguchi method, which was established by Genichi Taguchi in 1980s 11 , has been widely used as a systematic approach to optimize the design parameters under the given conditions 12 13 14 . This approach can significantly minimize the overall testing time and the experimental costs 12 13 14 15 16 . By using the specially designed orthogonal array, the optimum experimental conditions can be determined 13 .…”

Section: Methodsmentioning

confidence: 99%

Self-assembly modified-mushroom nanocomposite for rapid removal of hexavalent chromium from aqueous solution with bubbling fluidized bed

Liu

Chen

et al. 2016

Sci Rep

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A self-assembled modified Pleurotus Cornucopiae material (SMPM) combined with improved Intermittent Bubbling Fluidized Bed (IBFB) was investigated to remove the hexavalent chromium ions in aqueous solution. After the modification, the powder-like raw material gradually self-assembled together to SMPM, which had crinkly porous structure, improved the Cr-accommodation ability in a sound manner. Optimized by Taguchi method, Cr(VI) removal efficiency was up to 75.91% and 48.01% for 100 mg/L and 500 mg/L initial concentration of Cr(VI), respectively. Results indicated that the metal removal was dependent on dosage of adsorbent, particle diameter and treatment time. The experimental data obtained from the biosorption process was successfully correlated with Freundlich isotherm model. Thermodynamic study indicated the endothermic nature of the process. The results confirmed that self-assembly modified Pleurotus Cornucopiae material could be applied for the removal of heavy metal from wastewater in continuous fluidized bed process.

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Section: Methodsmentioning

confidence: 99%

Self-assembly modified-mushroom nanocomposite for rapid removal of hexavalent chromium from aqueous solution with bubbling fluidized bed

Liu

Chen

et al. 2016

Sci Rep

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“…[28][29][30] Different from the reported works, the Ni-Co film developed in our previous works is an alloy type of protein chip with bimetallic elements to enhance the specific binding capability with the histidine (His). [31][32][33] Therefore, the immobilization of different functional proteins with a histidine tag attached becomes feasible. To test the performance of this proposed droplet-based immunoassay chip, two biomaterials were adopted as examples of bioassays: tumor suppressor p53 and kinase ERK1.…”

Section: Introductionmentioning

confidence: 99%

Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

et al. 2020

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“…In our previous work, a protein chip with a Ni-Co layer fabricated by electrodeposition was developed [8][9][10]. It is an alloy-film type of protein chip.…”

Section: Introductionmentioning

confidence: 99%

Label‐free immunosensor based on interdigitated electrodes with Ni–Co nanowires

Siao

Chang

Shih

2018

Micro & Nano Letters

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This work presents a label-free immunosensor consisting of interdigitated electrodes and Ni-Co nanowires (NWs) to determine the relative amount of analyte, using electroanalytical measurement. Interdigitated electrodes were fabricated by the conventional printed circuit board treatment and Ni-Co NWs were synthesised by the electrodeposition method using a porous anodic alumina oxide membrane as the template. The experimental results showed that Ni-Co NWs can provide specific binding with histidine-tagged capture agent based on the method of immobilised metal affinity chromatography. Due to the large surface-to-volume ratio of NWs, this immunosensor had 2.6-fold the immobilisation ability of flat Ni-Co film to absorb histidine-tagged capture agent. Moreover, this immunosensor exhibited sensitivity in detecting low amounts of analyte. The electrical responses of the electroanalytical measurement of analyte, from 1000 to 625 ng/mL, could be discriminated; however, the fluorescence intensities became indiscriminative. This label-free immunosensor has the potential to immobilise various capture agents through the use of histidine for different analyte analyses.

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Protein microarray chip with Ni–Co alloy coated surface

Cited by 20 publications

References 28 publications

Self-assembly modified-mushroom nanocomposite for rapid removal of hexavalent chromium from aqueous solution with bubbling fluidized bed

Self-assembly modified-mushroom nanocomposite for rapid removal of hexavalent chromium from aqueous solution with bubbling fluidized bed

Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

Label‐free immunosensor based on interdigitated electrodes with Ni–Co nanowires

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