Protein-mimetic peptide nanofibers: Motif design, self-assembly synthesis, and sequence-specific biomedical applications

Zhang, Wensi; Yu, Xiaoqing; Li, Yang; Su, Zhiqiang; Jandt, Klaus D.; Wei, Gang

doi:10.1016/j.progpolymsci.2017.12.001

Cited by 159 publications

(107 citation statements)

References 244 publications

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“…Various self-assembling peptide nanofibers have been employed as advanced materials in different biomedical applications. [16] For example, peptide and protein amyloids have been widely used for medical imaging, tissue engineering, and drug delivery. [17] Hybrids based peptide nanofibers have been utilized for targeting and imaging tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme‐Induced Local Retention Properties

Bellat

Ting

Southard

et al. 2018

Adv Funct Materials

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An effective tumoral delivery system should show minimal removal by the reticuloendothelial system (RES), promote tumor uptake and penetration, and minimize on-site clearance. This study reports the design and synthesis of advanced self-assembling peptide nanofiber precursor (NFP) analogues. The peptidic nature of NFP offers the design flexibility for on-demand customization with imaging agents and surface charges while maintaining a set size, allowing for real-time monitoring of kinetic and dynamic tumoral delivery by multimodal fluorescence/positron emission tomography/computed tomography (fluo/PET/CT) imaging, for formulation optimization. The optimized glutathione (GSH)-NFP displays a reduced capture by the RES as well as excellent tumor targeting and tissue invasion properties compared to naive NFP. Inside a tumor, GSH-NFP can structurally transform into ten times larger interfibril networks, serving as in situ depot that promotes weeks-long local retention. This nanofiber, which can further be designed to release the active pharmacophores within a tumor microenvironment, displays a superior therapeutic efficacy for inhibiting disease progression and improving the survival of animals bearing triple-negative breast cancer tumors compared to free drug and liposome formulation of the drug, in addition to a favorable toxicity profile.

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Section: Introductionmentioning

confidence: 99%

Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme‐Induced Local Retention Properties

Bellat

Ting

Southard

et al. 2018

Adv Funct Materials

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“…Therefore, the use of biomolecules in NP syntheses has received attention as a potential 'green synthesis' vehicles for the morphological and functional regulation of inorganic nanomaterials. [3][4][5] Amongst the plethora of available biological molecules, peptides have attracted particular attention as valuable tools in bionanotechnology, [6][7][8][9][10][11][12] because of their diverse functionalities and, with certain peptides, the potential for specic binding to particular materials (both organic and inorganic). [13][14][15] Various peptides have been utilised in the functionalisation of nanomaterials, 13,16,17 the control of NP synthesis, 18,19 as biotemplating for morphological control, 20 and biocatalysts for the NP crystalisation.…”

mentioning

confidence: 99%

Rational screening of biomineralisation peptides for colour-selected one-pot gold nanoparticle syntheses

et al. 2019

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“…These results demonstrated that the endocytic uptake mechanisms involved in CaP nanoparticles were through the clathrin-mediated and caveolae-mediated cellular uptake pathway, but mainly in the clathrin-mediated pathway which subsequently delivered cargo into the endosome and then transferred into the lysosome, which led to DNA degradation and low transfection efficiency [13,18,19]. Thus, it was very crucial to alter the cellular uptake pathway and subsequently change the intracellular profiles for improving the efficacy of gene vectors.…”

Section: Endocytic Mechanisms Involved In Cap-ma Non-viral Vectorsmentioning

confidence: 96%

“…To date, numerous researchers focused on the design and construction of gene delivery vectors and made attempts to address the challenges. As for the non-viral delivery vectors, they often suffer insufficient performance due to poor transfection efficiency, relatively high toxicity, inadequate cellular uptake and gene degradation in endosome or lysosome, which significantly hampers the application in the clinic [1,[12][13][14]. Viral delivery vectors possess innate machinery to overcome cellular barriers, however, non-viral delivery vectors require great effort to rationally design to overcome these barriers.…”

Section: Introductionmentioning

confidence: 99%

Enhance transgene responses through improving cellular uptake and intracellular trafficking by bio-inspired non-viral vectors

Jia

et al. 2020

J Nanobiotechnol

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Background: Gene therapy remains a significant challenge due to lots of barriers limiting the genetic manipulation technologies. As for non-viral delivery vectors, they often suffer insufficient performance due to inadequate cellular uptake and gene degradation in endosome or lysosome. The importance of overcoming these conserved intracellular barriers is increasing as the delivery of genetic cargo. Results: A surface-functionalized non-viral vector involving the biomimetic mannitol moiety is initiated, which can control the cellular uptake and promote the caveolae-mediated pathway and intracellular trafficking, thus avoiding acidic and enzymatic lysosomal degradation of loaded gene internalized by clathrin-mediated pathway. Different degrees of mannitol moiety are anchored onto the surface of the nanoparticles to form bio-inspired non-viral vectors and CaP-MA-40 exhibits remarkably high stability, negligible toxicity, and significantly enhanced transgene expression both in vitro and in vivo. Conclusions: This strategy highlights a paradigmatic approach to construct vectors that need precise intracellular delivery for innovative applications.

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Protein-mimetic peptide nanofibers: Motif design, self-assembly synthesis, and sequence-specific biomedical applications

Cited by 159 publications

References 244 publications

Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme‐Induced Local Retention Properties

Functional Peptide Nanofibers with Unique Tumor Targeting and Enzyme‐Induced Local Retention Properties

Rational screening of biomineralisation peptides for colour-selected one-pot gold nanoparticle syntheses

Enhance transgene responses through improving cellular uptake and intracellular trafficking by bio-inspired non-viral vectors

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