Protein molecularly imprinted polyacrylamide membrane: for hemoglobin sensing

Wu, Shouguo; Tan, Wenguang; Xu, Haihong

doi:10.1039/c0an00191k

Cited by 57 publications

(25 citation statements)

References 26 publications

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“…Furthermore, the great flexibility of the protein structure means that it should be polymerized under almost-native conditions so that its shape would be preserved and the binding site accurately designed. Acrylamide matrices meet these criteria [35][36][37][38]. They polymerize under mild conditions and carry uncharged functions that establish hydrogen bonds and dipole-dipole interactions with Myo [6].…”

Section: Chemical Assembly Of the Molecularly Imprinted Materialsmentioning

confidence: 99%

Electrochemical biosensor based on biomimetic material for myoglobin detection

Moreira

Dutra

Noronha

et al. 2013

Electrochimica Acta

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a b s t r a c tA novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVC COOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification.The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 g mL −1 , of positive slope 6.50 ± 1.48 (k mL g −1 ). The limit of detection was 2.25 g mL −1 . Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 g mL −1 . A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 A mL g −1 . MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.

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Section: Chemical Assembly Of the Molecularly Imprinted Materialsmentioning

confidence: 99%

Electrochemical biosensor based on biomimetic material for myoglobin detection

Moreira

Dutra

Noronha

et al. 2013

Electrochimica Acta

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“…Future works with electrosynthesized pAPBA MIPs for glycoprotein recognition should consider prior immobilization of the template glycoprotein, which was found essential in obtaining satisfactory selectivity against other glycoproteins [76]. Beside biocompatibility, a wide variety of functional groups and extensive knowledge about the formed polymers motivated the use of dopamine [80,81] and acrylamide [57,82] for protein MIPs.…”

Section: Functional Monomers For the Electrosynthesis Of Protein Mipsmentioning

confidence: 99%

Electrosynthesized molecularly imprinted polymers for protein recognition

Erdőssy

Horváth²,

Yarman

et al. 2016

TrAC Trends in Analytical Chemistry

155

133

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“…The results demonstrated that the sensor possessed not only a short response time, but also a good selectivity and binding capacity for 4-nitrophenol, which enabled the sensor with higher current response than that of non-imprinted polymer, traditional MIP, and reduced graphene oxide. Electropolymerization of monomer in the presence of protein is the favorite method for MIP based electrochemical sensors preparation to recognize and detect template protein [17][18][19]. In recent years, dopamine (DA) has attracted great interests due to its unique property of selfpolymerization in weak alkaline solution on most organic and inorganic materials [20,21].…”

Section: Introductionmentioning

confidence: 99%

Recognition and determination of bovine hemoglobin using a gold electrode modified with gold nanoparticles and molecularly imprinted self-polymerized dopamine

Fan

Dai

et al. 2015

Microchim Acta

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A molecularly imprinted polymer (MIP) was prepared by self-polymerization of dopamine in the presence of bovine hemoglobin (BHb) and then deposited on the surface of an electrode modified with gold nanoparticles (AuNPs). Scanning electron microscopy, cyclic voltammetry, and differential pulse voltammetry were employed to characterize the modified electrode using the hexacyanoferrate redox system as an electroactive probe. The effects of BHb concentration, dopamine concentration, and polymerization time were optimized. Under optimized conditions, the modified electrode selectively recognizes BHb even in the presence of other proteins. The peak current for hexacyanoferrate, typically measured at + 0.17 V (vs. SCE), depends on the concentration of BHb in the 1.0×10 −11 to 1.0×10 −2 mg mL −1 range. Due to the ease of preparation and tight adherence of polydopamine to various support materials, the present strategy conceivably also provides a platform for the recognition and detection of other proteins.

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Protein molecularly imprinted polyacrylamide membrane: for hemoglobin sensing

Abstract: A hemoglobin sensor based on a molecularly imprinted polymer-modified electrode has been fabricated by electrochemically induced redox polymerization of acrylamide in the presence of hemoglobin.

Cited by 57 publications

References 26 publications

Electrochemical biosensor based on biomimetic material for myoglobin detection

Electrochemical biosensor based on biomimetic material for myoglobin detection

Electrosynthesized molecularly imprinted polymers for protein recognition

Recognition and determination of bovine hemoglobin using a gold electrode modified with gold nanoparticles and molecularly imprinted self-polymerized dopamine

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