Protein patterns and superoxide dismutase activity in non-mycorrhizal and arbuscular mycorrhizalPisum sativum L. plants

Arines, J.; Quintela, Manuela; Vilariño, A.; Palma, José M.

doi:10.1007/bf02185479

Cited by 30 publications

(27 citation statements)

References 22 publications

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“…However, very little is known about the complex gene network involved in the regulation of ROS homeostasis in AM, that is, the mechanisms controlling the balance between the ROS-producing and ROS-scavenging enzymes. SOD activity was shown to increase in mycorrhizal roots (Palma et al 1993;Arines et al 1994;Ruiz-Lozano et al 1996), however, the contribution of each partner is still unknown. In this work, we characterized a G. intraradices gene encoding a CuZnSOD to provide new data on the role of this enzyme in ROS homeostasis in AM.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a CuZn superoxide dismutase gene in the arbuscular mycorrhizal fungus Glomus intraradices

et al. 2010

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To gain further insights into the mechanisms of redox homeostasis in arbuscular mycorrhizal fungi, we characterized a Glomus intraradices gene (GintSOD1) showing high similarity to previously described genes encoding CuZn superoxide dismutases (SODs). The GintSOD1 gene consists of an open reading frame of 471 bp, predicted to encode a protein of 157 amino acids with an estimated molecular mass of 16.3 kDa. Functional complementation assays in a CuZnSOD-defective yeast mutant showed that GintSOD1 protects the yeast cells from oxygen toxicity and that it, therefore, encodes a protein that scavenges reactive oxygen species (ROS). GintSOD1 transcripts differentially accumulate during the fungal life cycle, reaching the highest expression levels in the intraradical mycelium. GintSOD1 expression is induced by the well known ROS-inducing agents paraquat and copper, and also by fenpropimorph, a sterol biosynthesis inhibitor (SBI) fungicide. These results suggest that GintSOD1 is involved in the detoxification of ROS generated from metabolic processes and by external agents. In particular, our data indicate that the antifungal effects of fenpropimorph might not be only due to the interference with sterol metabolism but also to the perturbation of other biological processes and that ROS production and scavenging systems are involved in the response to SBI fungicides.

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Section: Discussionmentioning

confidence: 99%

Characterization of a CuZn superoxide dismutase gene in the arbuscular mycorrhizal fungus Glomus intraradices

et al. 2010

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“…Although we seem far from assigning any biological function to the symbiosis-specific polypeptides detected by 2D-PAGE, progress could be made using immunological probes obtained from SR genes revealed by differential display of mRNA populations . Furthermore, targeted approaches on the first functional proteins now clearly identified in AM symbiosis, such as the mycorrhiza-specific chitinase isoforms (DumasGaudot et al, 1994(2;Dassi et al, 1996, Pozo et al, 1996 or the mycorrhiza-superoxide dismutase isoforms Arines et al, 1994) could greatly improve our knowledge of molecular mechanisms governing the AM symbiosis.…”

Section: Up and Down Regulation Of Preexisting Polypeptidesmentioning

confidence: 99%

Detection of symbiosis‐related polypeptides during the early stages of the establishment of arbuscular mycorrhiza between Glomus mosseae and Pisum sativum roots

1997

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SUMMARYChanges in polypeptide contents following inoculation of roots of Pisum sativum L. (wild type cv. Frisson (myc^, nod^)) with the arbuscular mycorrhizal fungus Glomus mosseae were analysed by two-dimensional gel electrophoresis (2D-PAGE). A different polypeptide pattern was obtained in a mycorrhiza-resistant pea genotype P2 (myc", nod") inoculated with G. mosseae. In order further to characterize the polypeptide modifications detected and to provide evidence for some possible symbiosis-related (SR) proteins, a time course experiment, from appressoria formation to fully developed symbiosis, was carried out on two genotypes allowing fungal colonization: the wild type and its isogenic mutant P56 (myc^, nod"). The same experiment was done with the mycorrhiza-resistant pea genotype (myc", nod"). After G. mosseae inoculation, we characterized 12 additional polypeptides in the two mycorrhiza-compatible pea genotypes which were never observed in root extracts from the mycorrhiza-resistant mutant. Five polypeptides were first detected in the early stage of the symbiosis (5 d of inoculation) while others were observed later (8 d of inoculation). The induction and accumulation of these polypeptides seem to be more correlated to the establishment of the functional symbiosis than to the recognition stages and appressorium formation. Furthermore, none of the additional polypeptides were detected in the mycorrhiza-resistant pea genotype. This mutant was more characterized by a great repression of polypeptides. In addition, up-regulated and down-regulated polypeptides from the mycorrhiza-compatible genotypes were different from those of the mycorrhiza-resistant genotype.

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“…In arbuscular mycorrhizal (AM) associations, the accumulation of ROS and the induction of ROS-scavenging enzymes, such as superoxide dismutase (SOD), catalase (CAT), and POX, have been reported (Arines et al 1994;Salzer et al 1999;Blilou et al 2000;Lambais et al 2003;Fester and Hause 2005). In contrast, during the development of ECM symbioses, data detailing the involvement of the antioxidative system are still scarce.…”

Section: Introductionmentioning

confidence: 99%

Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius

Baptista¹,

Martins²,

Pais³

et al. 2007

Mycorrhiza

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Evidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal (ECM) establishment is lacking. In this paper, we evaluated ROS production and the activities of superoxide dismutase (SOD) and catalase (CAT) during the early contact of the ECM fungus Pisolithus tinctorius with the roots of Castanea sativa (chestnut tree). Roots were placed in contact with P. tinctorius mycelia, and ROS production was evaluated by determining the levels of H 2 O 2 and O 2 I − during the early stages of fungal contact. Three peaks of H 2 O 2 production were detected, the first two coinciding with O 2 I − bursts. The first H 2 O 2 production peak coincided with an increase in SOD activity, whereas CAT activity seemed to be implicated in H 2 O 2 scavenging. P. tinctorius growth was evaluated in the presence of P. tinctorius-elicited C. sativa crude extracts prepared during the early stages of fungal contact. Differential hyphal growth that matched the H 2 O 2 production profile with a delay was detected. The result suggests that during the early stages of ECM establishment, H 2 O 2 results from an inhibition of ROS-scavenging enzymes and plays a role in signalling during symbiotic establishment.

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Protein patterns and superoxide dismutase activity in non-mycorrhizal and arbuscular mycorrhizalPisum sativum L. plants

Cited by 30 publications

References 22 publications

Characterization of a CuZn superoxide dismutase gene in the arbuscular mycorrhizal fungus Glomus intraradices

Characterization of a CuZn superoxide dismutase gene in the arbuscular mycorrhizal fungus Glomus intraradices

Detection of symbiosis‐related polypeptides during the early stages of the establishment of arbuscular mycorrhiza between Glomus mosseae and Pisum sativum roots

Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius

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