Protein:protein aggregation induced by protein oxidation

Carbonylation of the cytoskeletal protein actin leads to aggregate formation

“…It was suggested, that one of these is actin which, in vitro, exhibits elevated susceptibility to carbonylation and can form covalent cross-linking as well as noncovalent aggregates [11]. Such actin susceptibility was also observed in rat spinal cord cells [12] and mussel muscular cells [13] and additional actin filament disruption and polymerization failure were also noted in the Caco-2 colonic cell line [14] and yeast [15] when submitted to oxidative challenge. The data support the point that actin is thus a protein with a major likelihood to oxidative modifications.…”

Relationship Between Protein Oxidation Markers and Oxidative Stress Biomarkers

“…Hence, there are many attempts to develop antioxidants that can cross the blood-brain barrier and decrease oxidative damage (Halliwell 2007) and of biopharmaceuticals that can counteract protein oxidation and precipitation-aggregation (Wang 2005). Aggregation of proteins is a common feature triggered by protein oxidation and it has been found "in vitro and "in vivo" being carbonylation a common feature (Mirzaei & Regnier 2008). Aggregation is manifest in globular proteins, because under stress conditions or proteolysis nonnative conformations can be adopted.…”

A computational method to predict carbonylation sites in yeast proteins

“…Furthermore, yeast has a small but well-defined genome. Lastly, yeast expresses numerous proteins that are orthologous to mammalian proteins (MacLean et al, 2001;Longo and Fabrizio, 2002;Mirzaei and Regnier, 2008). However, the yeast genome encodes for approximately 6000 proteins (Mirzaei and Regnier, 2006), and current experimental approaches used to identify carbonylation sites are expensive, time-consuming, and limited in protein processing abilities.…”