Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite

Ranguelova, Kalina; Chatterjee, Saurabh; Ehrenshaft, Marilyn; Ramirez, Dario C.; Summers, Fiona A.; Kadiiska, Maria B.; Mason, Ronald P.

doi:10.1074/jbc.m109.069054

Cited by 58 publications

(61 citation statements)

References 60 publications

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“…1A. From the EPR spectrum, the calculated isotropic hyperfine coupling constants are a N ¼14.57 G and a H ¼16.09 G, which are in agreement with previous reports [51]. In the absence of The level of sulfite is increased under various pathological conditions, including environmental exposure [2,3,25].…”

Section: Electron Paramagnetic Resonance (Epr) Measurementssupporting

confidence: 77%

“…It has been demonstrated that sulfite toxicity is due to the increased production of ROS in the mitochondria and inactivation of mitochondrial proteins [9]. Studies have also shown that sulfite radical is capable of damaging DNA/ RNA, lipids, and proteins [51,76,77,[95][96][97]. The increased production of oxidants can damage the mitochondrial proteins, lipids, DNA/RNA and this is implicated in several neurological diseases [86,91,98].…”

Section: Discussionmentioning

confidence: 99%

“…Studies have demonstrated that the sulfite radical further reacts with oxygen to form peroxymonosulfate and sulfate radicals, which are powerful oxidants, Scheme 3 [51]. However, the spin trap DMPO (100 mM) competes with oxygen and scavenges sulfite radical [51].…”

Section: Discussionmentioning

confidence: 99%

“…However, the spin trap DMPO (100 mM) competes with oxygen and scavenges sulfite radical [51]. In cells, reactive oxygen species such as H 2 O 2 are produced by the mitochondria under physiological and pathophysiological conditions [52].…”

Section: Discussionmentioning

confidence: 99%

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Sulfite Oxidase Activity of Cytochrome C: Role of Hydrogen Peroxide

Murugesan

Hemann

Cardounel

et al. 2015

Free Radical Biology and Medicine

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Section: Electron Paramagnetic Resonance (Epr) Measurementssupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Sulfite Oxidase Activity of Cytochrome C: Role of Hydrogen Peroxide

Murugesan

Hemann

Cardounel

et al. 2015

Free Radical Biology and Medicine

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“…Since the production of the anti-DMPO antibody [23], a number of protein radicals have been detected by DMPO-based IST [21, 32], including myoglobin [23, 34–36], hemoglobin [22, 35, 37–38], cytochrome c [39], neuroglobin [40], Cu, Zn-superoxide dismutase (SOD1) [41–43] and Mn-SOD [44], catalase [33], myeloperoxidase [45–46], eosinophil peroxidase [47], lactoperoxidase [48–49], microglobulin [50], albumin [43, 51], thyroid peroxidase (TPO)[25], α-lactalbumin [52] and Ras GTPase [53]. Oxidants used to generate these radicals included H 2 O 2 , peroxynitrite, Cu 2+ /H 2 O 2 , hypochlorous acid, tert -butylhydroperoxide, peroxymonocarbonate, and reactive species generated from ketoprofen plus UV-A, riboflavin plus UV-A irradiation and nano-TiO 2 plus UV-A irradiation.…”

Section: Ist Study Of Protein Radicals In Purified Proteinsmentioning

confidence: 99%

Immuno-spin trapping from biochemistry to medicine: Advances, challenges, and pitfalls. Focus on protein-centered radicals

Gomez-Mejiba

Zhai

Vedova

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

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BACKGROUND Immuno-spin trapping (IST) is based on the reaction of a spin trap with a free radical to form a stable nitrone adduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduct. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug-induced agranulocytosis, hepatotoxicity, cardiotoxicity, and ischemia/reperfusion, as well as in models of neurological, metabolic and immunological diseases. SCOPE OF THE REVIEW To critically evaluate advances, challenges, and pitfalls as well as the scientific opportunities of IST as applied to the study of protein-centered free radicals generated in stressed organelles, cells, tissues and animal models of disease and exposure. MAJOR CONCLUSIONS Because the spin trap has to be present at high enough concentrations in the microenvironment where the radical is formed, the possible effects of the spin trap on gene expression, metabolism and cell physiology have to be considered in the use of IST and in the interpretation of results. These factors have not yet been thoroughly dealt with in the literature. GENERAL SIGNIFICANCE The identification of radicalized proteins during cell/tissue response to stressors will help define their role in the complex cellular response to stressors and pathogenesis; however, the fidelity of spin trapping/ immuno-detection and the effects of the spin trap on the biological system should be considered.

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Cross‐talk Between (Hydrogen)Sulfite and Metalloproteins: Impact on Human Health

Maiti

2022

Chemistry A European J

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Sulfite is a potent toxic substance causing harm to multi-organ in human. Despite toxicity, it is widely used as preservative, anti-browning and anti-oxidant in foods, beverages, and pharmaceuticals, which cause easy admission of sulfite in human. Sulfite is also produced endogenously during the catabolism of cysteine and methionine. In vivo, the serum sulfite level at physiological range is strictly maintained by a molybdenum dependent sulfite oxidase (SO), which catalyzes sulfite to sulfate oxidation via a two-electron oxidation pathway. The loss of SO activity causes high serum sulfite level that fosters several diseases, including asthma, neurological dysfunction, birth defects, and heart diseases. The cytotoxicity of (bi)sulfite is implicated as sulfite radicals, which are generated by mainly heme-peroxidases via a oneelectron oxidation pathway. On the other hand, the toxic sulfite radicals are neutralized to sulfite by heme-globins. The enzymatic reduction of sulfite to sulfide is catalyzed by sulfite reductase, which contains an unusual metal cofactor, siroheme-[4Fe4S]-cluster. Overall, the interaction of sulfite with various metalloproteins in vivo is a close relation with human health. Therefore, this review describes the metabolic conversion of (bi)sulfite to sulfate, sulfite radical or sulfide via oxidation or reduction pathways by various metalloproteins (specially SOs, peroxidases, heme-globins, and sulfite reductases), and the potential applications of sulfite in biosensors/ biofuel cells, anti-browning, and advance oxidation process.

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Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite

Cited by 58 publications

References 60 publications

Sulfite Oxidase Activity of Cytochrome C: Role of Hydrogen Peroxide

Sulfite Oxidase Activity of Cytochrome C: Role of Hydrogen Peroxide

Immuno-spin trapping from biochemistry to medicine: Advances, challenges, and pitfalls. Focus on protein-centered radicals

Cross‐talk Between (Hydrogen)Sulfite and Metalloproteins: Impact on Human Health

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