Protein‐responsive imprinted polymers with specific shrinking and rebinding

Abstract

Stimuli-responsive protein imprinted polymers were obtained via a combination of molecular imprinting and reversible stimuli-responsive polymer using lysozyme or cytochrome c as template, N-isopropylacrylamide (NIPA) as major monomer, methacrylic acid (MAA) and acrylamide (AAm) as functional co-monomers, and N,N-methylenebisacrylamide (MBAAm) as crosslinker. The molecularly imprinted polymers (MIPs) can respond not only to external stimuli such as temperature and salt concentration, but also to the corresponding template protein with significant specific volume shrinking. This specific shrinking behavior was attributed to the synergistic effect of multiple-site weak interactions (electrostatic force, hydrogen bonding and hydrophobic interaction) and the cavity effect. The MIPs showed highly selective adsorption of template proteins with specific shrinking compared with the non-imprinted polymers. The results indicated that the MIPs seemed to change shape to accommodate the conformation of the template protein leading to the formation of a shape complementary cavity.

pH‐Sensitive Water‐Soluble Nanospheric Imprinted Hydrogels Prepared as Horseradish Peroxidase Mimetic Enzymes

“…[19] We have previously developed poly-N-isopropylacrylamide (pNIPAM)-based protein-imprinted polymer gels that can sensitively respond not only to external stimuli, such as temperature and salt concentration, but also to the corresponding template protein with significant volume shrinking. [20] However, the employment of a stimuli-responsive polymer in the preparation of a catalytic MIP with typical enzyme-like characteristics has thus far not yet been reported. So in this study, we attempt to improve the water solubility of the imprinted catalyst and to mimic the dual functions of natural enzymes by combining a stimuli-responsive hydrogel and a molecularly imprinted catalyst in the same material.…”

Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

“…Moreover, PNIPAAm hydrogels as matrices possess a degree of flexibility very similar to natural tissue, which ensures biocompatibility and conformational stability of the proteins. To date, there have been several works focusing on the design of protein-imprinted materials based on PNIPAAm [24][25][26][27].…”

Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique

“…Molecularly imprinting technology is an attractive mimetic approach to create specific binding sites complementary to the shape and functional group arrangement of template molecules [7]. Strategies to form MIPs have been used successfully for small molecules recognition [8][9][10][11].…”