Protein ROMP: Aqueous Graft-from Ring-Opening Metathesis Polymerization

Isarov, Sergey A.; Pokorski, Jonathan K.

doi:10.1021/acsmacrolett.5b00497

Cited by 64 publications

(62 citation statements)

References 31 publications

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“…Ring‐opening metathesis polymerization (ROMP) was also applied in the synthesis of protein–polymer bioconjugates. Isarov and Pokorski developed graft‐from chemistry based on ROMP ( Figure ) . In their approach, Grubbs' third generation catalyst was modified with PEGylated pyridyl groups, and then reacted with monomer‐modified lysozyme, forming a stable water‐soluble ROMP macroinitiator.…”

Section: Synthesis Of Polymer–protein Conjugatesmentioning

confidence: 99%

Section: Synthesis Of Polymer–protein Conjugatesmentioning

confidence: 99%

“…Isarov and Pokorski developed graft-from chemistry based on ROMP (Figure 3). [142] In their approach, Grubbs' third generation catalyst was modified with PEGylated pyridyl groups, and then reacted with monomer-modified lysozyme, forming a stable water-soluble ROMP macroinitiator. Subsequently, the protein macroinitiator was used to grow water-soluble norbornenes, resulting in the synthesis of high molecular weight protein-polymer conjugates.…”

Section: Grafting-from Approachmentioning

confidence: 99%

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Fabrication of Polymer–Protein Hybrids

Zhang

Zhao

2018

Macromol. Rapid Commun.

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Rapid developments in organic chemistry and polymer chemistry promote the synthesis of polymer-protein hybrids with different structures and biofunctionalities. In this feature article, recent progress achieved in the synthesis of polymer-protein conjugates, protein-nanoparticle core-shell structures, and polymer-protein nanogels/hydrogels is briefly reviewed. The polymer-protein conjugates can be synthesized by the "grafting-to" or the "grafting-from" approach. In this article, different coupling reactions and polymerization methods used in the synthesis of bioconjugates are reviewed. Protein molecules can be immobilized on the surfaces of nanoparticles by covalent or noncovalent linkages. The specific interactions and chemical reactions employed in the synthesis of core-shell structures are discussed. Finally, a general introduction to the synthesis of environmentally responsive polymer-protein nanogels/hydrogels by chemical cross-linking reactions or molecular recognition is provided.

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Section: Synthesis Of Polymer–protein Conjugatesmentioning

confidence: 99%

Section: Synthesis Of Polymer–protein Conjugatesmentioning

confidence: 99%

Section: Grafting-from Approachmentioning

confidence: 99%

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Fabrication of Polymer–Protein Hybrids

Zhang

Zhao

2018

Macromol. Rapid Commun.

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“…Furthermore, side chain functionality, incorporated either in the oxazoline monomer or through post‐polymerization modification has been exploited for the ‘grafting‐through’ modification of polymer chains and particles . The latest generation of the Grubb's catalyst has also led to the use of ROMP in the preparation of protein‐polymer conjugates via both ‘grafting‐to’ and ‘grafting‐from’ strategies . The resulting polymers composed of norbornene‐based monomers exhibit markedly different properties compared to the conventional (meth)acrylate derived polymers traditionally employed.…”

Section: Synthetic Approaches To Covalently‐linked Protein/peptide‐pomentioning

confidence: 99%

“…[46] The latest generation of the Grubb's catalyst has also led to the use of ROMP in the preparation of protein-polymer conjugates via both 'grafting-to' and 'graftingfrom' strategies. [47,48] The resulting polymers composed of norbornenebased monomers exhibit markedly different properties compared to the conventional (meth)acrylate derived polymers traditionally employed.…”

Section: Synthetic Approaches To Covalently-linked Protein/ Peptide-pmentioning

confidence: 99%

Synthesis and Applications of Protein/Peptide-Polymer Conjugates

Wilson

2017

Macromol. Chem. Phys.

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Recent advances in synthetic methodologies have brought us closer than ever to the precision conferred by nature. For example, the control possible in reversible deactivation radical polymerization enables us to design and synthesize macromolecules with unprecedented control over not only the polymer chain ends, but also the side chain functionality. Furthermore, this functionality can be exploited to afford chemical modification of peptides and proteins, with ever‐improving site‐specificity, yielding a range of well‐defined protein/peptide‐hybrid materials. Such materials benefit from the amalgamation of the properties of proteins/peptides with those of the synthetic (macro)molecules in question. Here, the latest developments in the synthesis of functional polymers and their use for preparation of well‐defined protein/peptide‐polymer conjugates will be discussed, with particular attention focused on modulating the stability, efficacy and/or administration of therapeutic peptides.

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Weaving Enzymes with Polymeric Shells for Biomedical Applications

Blum

et al. 2021

Advanced Materials

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the most common administration method. However, the sizes of most enzymes are smaller than the kidney filtration threshold (50-60 kDa), resulting in fast renal clearance and short half-lives. [5] Also, limited by its hydrophilicity and large molecular weight, it is difficult for exogenous enzyme macromolecules to pass through the cell membrane to effectively catalyze intracellular target reactions. [6] To date, most of the clinical protein therapeutics were explored toward extracellular targets owing to the low translocation efficiency. [7] However, for enzyme therapeutics, especially for the enzyme replacement therapy, intracellular delivery is considered critical for effective therapy. [8] Typically, even if a number of enzymes are transfected into

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Protein ROMP: Aqueous Graft-from Ring-Opening Metathesis Polymerization

Cited by 64 publications

References 31 publications

Fabrication of Polymer–Protein Hybrids

Fabrication of Polymer–Protein Hybrids

Synthesis and Applications of Protein/Peptide-Polymer Conjugates

Weaving Enzymes with Polymeric Shells for Biomedical Applications

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