“…These regions correspond predominantly to loops, insertions of any length, and non-conserved side chains. The most significant part of comparative modeling termed as model or loop refinement is done using either of techniques, fragment-guided molecular dynamic simulation (FG-MD) [19], GalaxyRefine [20,21], 3Drefine [22][23][24], protein structure refinement via molecular dynamics (PREFMD) [25], Errat-guided LM via MODELLER [7] and molecular dynamics simulations (MDS) via AMBER16 [26] However, none of them describes the effectiveness of one method over the other in terms of model validation parameters, required experimental time and tediousness of each method during refinement procedure. Identifying the most effective technique can help in selecting the best refinement method, possibly reducing the required computational time and accelerate the process for refinement of homology model.…”