Protein synthesis in liposomes with a minimal set of enzymes

Murtas, Giovanni; Kuruma, Yutetsu; Bianchini, Paolo; Diaspro, Alberto; Luisi, Pier Luigi

doi:10.1016/j.bbrc.2007.07.201

Cited by 140 publications

(105 citation statements)

References 20 publications

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“…Here, either a simple self-replicating molecule or an enzymatic system is encapsulated by a self-reproducing micelle 26,27 or vesicle 28,29 . Developing a chemical protocellular model system of this kind has been a research goal for several decades; the state-of-the-art was recently and elegantly exemplified by Kurihara et al 30 , who amplified DNA by the PCR within self-reproducing vesicles and demonstrated a coupling between the replication of nucleotides and lipids.…”

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confidence: 99%

Physical autocatalysis driven by a bond-forming thiol–ene reaction

2014

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Autocatalysis has been extensively studied because it is central to the propagation of living systems. Chemical systems which self-reproduce like living cells would offer insight into principles underlying biology and its emergence from inanimate matter. Protocellular models feature a surfactant boundary, providing compartmentalization in the form of a micelle or vesicle and any model of the emergence of cellular life must account for the appearance, and evolution of, such boundaries. Here, we describe an autocatalytic system where two relatively simple components combine to form a more complex product. The reaction products aggregate into micelles that catalyse molecular self-reproduction. Study of the reaction kinetics and aggregation behaviour suggests a mechanism involving micelle-mediated physical autocatalysis and led to the rational design of a second-generation system. These reactions are driven by irreversible bond formation and provide a working model for the autocatalytic formation of protocells from the coupling of two simple molecular components.

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confidence: 99%

Physical autocatalysis driven by a bond-forming thiol–ene reaction

2014

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“…Starting from 2006, the use of PURE system for synthesizing a protein inside liposomes has become the benchmark for SSMCs construction (Sunami et al, 2006;Murtas et al, 2007), even if the yield of produced protein is typically one third when compared with cell extracts (Hillebrecht & Chong, 2008). Interestingly, we can look at the PURE system as a synthetic biology tool from the viewpoint of "standard biological parts" (http://partsregistry.org/Main_Page).…”

Section: The Technology For Building Minimal Cellsmentioning

confidence: 99%

Advances in Minimal Cell Models: a New Approach to Synthetic Biology and Origin of Life

Stano¹

2011

Progress in Molecular and Environmental Bioengineering - From Analysis and Modeling to Technology Applications

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“…For a variety of reasons, it would be extremely interesting to encapsulate these components within artificial cell-like compartments (Szostak et al, 2001;Pohorille and Deamer, 2002;Luisi et al, 2006;Forster and Church, 2006;Murtas et al, 2007). Due to the limited diffusion space, reaction kinetics could be faster and reaction products would not be lost by diffusion-an attractive aspect for nanoscale synthesis.…”

Section: Artificial Cellsmentioning

confidence: 99%