Protein synthesis in muscle of mature sheep

Bryant, D. T. W.; Smith, Richmond W.

doi:10.1017/s0021859600054447

Cited by 25 publications

(19 citation statements)

References 18 publications

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“…Arnal (1977) found a substantial decline in synthetic rate between 1 and 16 weeks of age in lambs and also observed differences between red and white muscles. Bryant & Smith (1982a) measured synthesis in several muscles of mature sheep and they observed lower rates than Arnal (1977) with little difference between most muscles. Vastus intermedius muscle in the hind leg, however, had higher rates of synthesis than other muscles.…”

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confidence: 83%

Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique

1987

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There is little information on rates of protein synthesis in individual muscles in ruminant animals. Skeletal muscle is the largest single tissue and, as such, has a considerable effect on nitrogen transactions in the body. Arnal (1977) found a substantial decline in synthetic rate between 1 and 16 weeks of age in lambs and also observed differences between red and white muscles. Bryant & Smith (1982a) measured synthesis in several muscles of mature sheep and they observed lower rates than Arnal (1977) with little difference between most muscles. Vastus intermedius muscle in the hind leg, however, had higher rates of synthesis than other muscles. Here we report rates of protein synthesis in individual muscles in rapidly growing lambs approximately 12 weeks of age. The major back muscle, the longissimus dorsi, and two hind leg muscles, the vastus lateralis and the vastus intermedius, were chosen for study.

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confidence: 83%

Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique

1987

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“…Indeed, several studies report that the rate of protein degradation is increased in animals fed diets which result in an acetate fermentation (Bryant & Smith 1982;Abdul-Razzaq & Bickerstaffe 1989).…”

Section: Introductionmentioning

confidence: 99%

Diet-induced variation in acetate metabolism of ovine perirenal adipose tissuein vitro

Scollan

Jessop

1995

J. Agric. Sci.

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SUMMARYSixteen Blackface wether lambs (25–27 kg) were fed for 9 weeks on diets based on either barley (B) or sugarbeet pulp (S), with either a low or high protein content. A constant amount of metabolizable energy (ME) was given at twice maintenance level. At one stage the animals were fed frequently and blood was sampled over a 24 h period. Plasma was assayed for acetate, glucose and insulin. After slaughter, the metabolism of acetate to CO2 and lipid was investigated in perirenal adipocytes in vitro.Frequent feeding helped to achieve near-steady-state conditions. Carbohydrate source influenced plasma acetate (P < 0·08) but not glucose or insulin. Level of protein in the diet influenced plasma acetate, higher concentrations of acetate were associated with lower levels of protein (P < 0·01). Acetate oxidation and incorporation into lipid was influenced by the concentrations of acetate, glucose and insulin in the incubation media (P < 0·001). Acetate oxidation was influenced by carbohydrate source (P < 0·05), whereas level of protein in the diet affected acetate incorporation into lipid (P < 0·05).These results show how the balance of nutrients provided to a ruminant for metabolism may influence selected plasma parameters and the metabolism of acetate by adipose tissue, which may help to explain the reduced efficiency of ME utilization from more fibrous diets.

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“…We are unaware of other experiments in which the rates of muscle protein synthesis in different anatomical regions of cattle have been compared. It has been shown however that, in sheep, the rate of synthesis in the vastus intermedius muscle in the hind leg is higher than in the neighbouring vastus lateralis and in the longissimus dorsi in the back (Bryant & Smith 1982;. The comparative slaughter experiment of Wood et al (1986) showed that, in steers treated with a combined implant of trenbolone acetate and oestradiol, there was preferential deposition of protein in the forequarter muscles compared with the muscles of the back and .…”

Section: Discussionmentioning

confidence: 99%

Whole-body and tissue protein synthesis in steers losing weight on a low-protein roughage diet: the effect of trenbolone acetate

Hunter

Magner

1990

J. Agric. Sci.

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S U M M A R YSix Brahman (Bos indicus) steers implanted with 300 mg trenbolone acetate and six similar nonimplanted steers were fed a low-quality, low-protein roughage diet at 10 g dry matter/kg live weight. They were housed in individual pens for 27 days in 1987 before being placed in metabolism crates for measurement of digestibility, nitrogen retention, and the rate of protein synthesis in the whole body and fractional rate in three muscles and hide.The rate of weight loss of steers treated with trenbolone acetate was significantly (P < 005) less than that of controls (-0-34 v. -0-56 kg/day). Treated steers excreted significantly (P < 005) less 3-methylhistidine (128 v. 202 umol) and urea N (0-5 v. 1 -6 g/day) in urine. There was a more favourable nitrogen retention in animals implanted with trenbolone acetate (-10 v. -12g/day) but this difference was not significant.Whole-body protein synthesis, calculated from plasma leucine flux, was 511 and 508 g/day in treated and control steers, respectively. Treatment also had no significant effect on the fractional rate of protein synthesis, in skeletal muscle or in hide. The fractional rate of synthesis in muscle was < 1 %/day and in hide < 2%/day. These results are discussed in relation to the protein and energy metabolism of undernourished cattle. I N T R O D U C T I O Na su b m ai n tenance diet and related changes in protein metabolism to known changes in energy metabolism Beef production in seasonally dry areas follows a (Hunter & Vercoe 1987) associated with trenbolone pattern of weight gain during the wet season and zero acetate treatment. gain or weight loss during the dry season, resulting in A possible complication with trenbolone acetate is small annual gains. Improvements in production the differential effect on protein transactions in could result from an increase in gain during the wet or different muscles. It can be calculated from the a decrease in loss during the dry season. There is comparative slaughter experiment of Wood et al. evidence that the anabolic agent trenbolone acetate (1986) that a combined implant of trenbolone acetate reduces energy expenditure in cattle (Hunter & and oestradiol increased protein deposition in the Vercoe 1987), and thus has the potential to reduce neck and shoulder by an average of c. 20% and in the weight loss in the dry season. It has been suggested vertebral column and hindquarter muscles by only that part of the mode of action of trenbolone acetate c. 10%. In this paper, the effect of trenbolone acetate in the weight gain of female rats (Vemon & Buttery on protein synthesis in the whole body, in the hide 1976, \91Sa,b)and female lambs (Sinnett-Smith et al. and in three muscles was measured, and the calculated 1983) is its effect on reducing muscle protein synthesis energy costs of protein synthesis were related to and breakdown. As the reduction in breakdown is energy expenditure in the whole animal. The muscles greater than the reduction in synthesis, the net result were the trapezius in the shoulder, the longiss...

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Protein synthesis in muscle of mature sheep

Cited by 25 publications

References 18 publications

Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique

Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique

Diet-induced variation in acetate metabolism of ovine perirenal adipose tissuein vitro

Whole-body and tissue protein synthesis in steers losing weight on a low-protein roughage diet: the effect of trenbolone acetate

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