Protein synthesis in pox-infected cells treated with interferon

Bodo, G.; Scheirer, W; Suh, Martha; Schultze, B.; Horak, Ivan D.; Jungwirth, C.

doi:10.1016/0042-6822(72)90354-6

Cited by 25 publications

(15 citation statements)

References 14 publications

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“…Most evidence obtained with poxvirus-infected chick embryo fibroblasts supports the notion that in interferon-treated cells virus-specific mRNA translation is impaired [5,6]. Previous studies on the molecular mechanism of interferon action have been largely limited to the early phase of poxvirus infection.…”

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confidence: 62%

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Inhibition of Poxvirus‐Specific Functions Induced in Chick‐Embryo Fibroblasts by Treatment with Homologous Interferon

Osterhoff

Jäger

Jungwirth

et al. 1976

European Journal of Biochemistry

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Poxvirus‐infected primary chick embryo fibroblasts have been used as a model system to study the mechanism of the antiviral activity of homologous interferon. The effect of pretreatment of cells with 10–300 units/ml of interferon on the synthesis of cowpox and vaccinia WR virus‐specific proteins was tested in the ‘early’ and ‘late’ phase of infection. [35S]Methionine‐labelled virus‐specific proteins were examined after separation from host cell proteins by sodium dodecylsulfate acrylamide gel electrophoresis followed by autoradiography. Synthesis of individual poxvirus‐specific proteins was affected to varying degrees by interferon treatment of the cells. Some ‘late’ viral proteins were synthesized at a reduced rate even if the cells had been treated with only 10 units/ml of interferon. On the other hand, even after pretreatment with high doses of interferon (1000 units/ml) synthesis of certain virus‐specific proteins could not be completely inhibited. In the late stage of infection with strain vaccinia WR or cowpox virus, a protective effect of interferon treatment on host cell protein synthesis could be detected. Vaccinia‐WR‐specific DNA synthesis is fairly resistant to interferon pretreatment, thereby making it possible to analyze the transcription program of virus‐specific RNA in the ‘late’ phase of infection in cells treated with low doses of interferon. All vaccinia‐WR‐specific RNA species transcribed in the ‘late’ phase of infection were detectable in chick embryo fibroblasts pretreated with 100 units ml of interferon which resulted in strong inhibition of the synthesis of several ‘late’ viral proteins. Cowpox‐virus‐infected primary chick embryo fibroblasts developed a distinct cytopathic effect at late stages of infection but vaccinia‐WR‐infected cells did not. Morphologically this cytopathic effect was reduced in interferon‐treated cowpox‐infected chick embryo fibroblasts.

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confidence: 62%

“…Primary chick embryo fibroblasts have been used extensively to study the mechanism of the antiviral effect on various animal viruses mainly because of the availability of highly purified chick interferon preparations. Furthermore, anti-cellular effects are slight under conditions where macromolecule synthesis and virus growth are drastically affected [5].…”

mentioning

confidence: 99%

Inhibition of Poxvirus‐Specific Functions Induced in Chick‐Embryo Fibroblasts by Treatment with Homologous Interferon

Osterhoff

Jäger

Jungwirth

et al. 1976

European Journal of Biochemistry

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“…Depending on the interferon concentration used to induce the antiviral state, an increasing number of infected cells attached to the petri dish and the culture can grow to confluency in interferon-containing medium (unpublished observations). Cowpox virus was used for the study of the interferon effect on poxvirus-specific DNA-binding protein synthesis because the virus strain is, compared to vaccinia WR, slightly more interferon-sensitive [3,6]. Some studies were also done with vaccinia WR.…”

Section: Sensitiuity Of'synthesis Of' Cowpox-virus-induced Early Dnabmentioning

confidence: 99%

“…This shows that the synthesis of this specific group of cellular proteins in chick cells is also not turned off by viral infection. The absence of a general inhibition of cellular protein synthesis after poxvirus infection of chick cells has been described previously [3]. The [35S]methionine-labeled protein fraction from infected chick cells and L-929 cells not binding to the DNA-cellulose column under these conditions was also analyzed by SDSjPAGE (Fig.…”

Section: Isolation Of Dna-binding Proteins From Vaccinia-virus-infecmentioning

confidence: 99%

“…It has been established that in cells pretreated with homologous interferon, formation of mature virus particles and viral protein synthesis are inhibited in a concentration-depcndent manner. Only an incomplete analysis of early vaccinia-virus-specific protein synthesis in chick embryo fibroblasts treated with interferon has been carried out because of the low viral titer in these cells and because host cell protein synthesis is not switched off early after infection [3]. Therefore the detection of early viral proteins is more difficult than in many other poxviruse-infected cells.…”

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Isolation of Early Viral Proteins from Poxvirus-Insfected Chick Embryo Fibroblasts by DNA-Cellulose Chromatography and Inhibition of Their Synthesis by Chicken Interferon

Rösel

Jungwirth

1983

Eur J Biochem

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Up to seven early poxvirus-specific proteins have been isolated from vaccinia-WR-infected and cowpox-virusinfected chick embryo fibroblasts by affinity chromatography on native DNA-cellulose columns. The proteins have been characterized by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis and by nonequilibrium pH-gradient electrophoresis. The molecular weights of the viral proteins were determined by comparison with proteins of known molecular weight and are comparable to several of the vaccinia-WR-specific DNA-binding proteins isolated previously from infected L-929 cells by Solosky J. M., Esteban M. and Holowczak J. A. [ J . Vim/. 25, 263 -273 (1978)l. The viral proteins binding reversibly to native DNA have been classified as immediate early viral gene products. Synthesis of cowpox-virus-induced early DNA-binding proteins is inhibited in chick cells pretreated with homologous interferon at a concentration of 500 -1000 units/ml.The antiviral effect of interferon is directed against both cytocidal and tumor viruses. The primary target in the replication of cytocidal viruses seems to be the viral gene expression. Despite extensive studies on virus-infected cells and cell-free systems prepared from interferon-treated cclls, the mcchanism of the inhibition of viral protein synthesis, the connection to cellular alterations by interferon and the molecular basis of the discrimination belween viral and cellular functions are not yet completely understood [I].The replication cycle of poxviruses in chick embryo fibroblasts has been previously used to study the antiviral activities of interferon [2]. It has been established that in cells pretreated with homologous interferon, formation of mature virus particles and viral protein synthesis are inhibited in a concentration-depcndent manner. Only an incomplete analysis of early vaccinia-virus-specific protein synthesis in chick embryo fibroblasts treated with interferon has been carried out because of the low viral titer in these cells and because host cell protein synthesis is not switched off early after infection [3]. Therefore the detection of early viral proteins is more difficult than in many other poxviruse-infected cells.In this report we describe the isolation of early viral proteins in vaccinia-WR-infected and cowpox-virus-infected chick embryo fibroblasts by affinity chromatography on native DNA. They were preliminary characterized by onedimensional and two-dimensional separation techniques and several of them classified as immediate early viral proteins. Previous studies [2] of the interferon effect on viral gene ~~

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Reproduction of Poxviruses

Moss

1974

Reproduction

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Protein synthesis in pox-infected cells treated with interferon

Cited by 25 publications

References 14 publications

Inhibition of Poxvirus‐Specific Functions Induced in Chick‐Embryo Fibroblasts by Treatment with Homologous Interferon

Inhibition of Poxvirus‐Specific Functions Induced in Chick‐Embryo Fibroblasts by Treatment with Homologous Interferon

Isolation of Early Viral Proteins from Poxvirus-Insfected Chick Embryo Fibroblasts by DNA-Cellulose Chromatography and Inhibition of Their Synthesis by Chicken Interferon

Reproduction of Poxviruses

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