Proteins in Art, Archaeology, and Paleontology: From Detection to Identification

Dallongeville, Sophie; Garnier, Nicolas; Rolando, Christian; Tokarski, Caroline

doi:10.1021/acs.chemrev.5b00037

Cited by 141 publications

(80 citation statements)

References 607 publications

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“…While a method, called peptide mass fingerprint, is widely known for the straightforward identification of proteins in the biomedical field39 and it has been successfully adapted to the study of proteins in samples from cultural heritage40, the identification of plant gums is still challenging and this paper aims to exceed these obstacles by adapting metabolomics-like strategies41. The strategy consists of the partial enzymatic digestion of the natural polymers with glycoside hydrolases followed by MALDI-TOF MS and MS/MS analysis of the released oligosaccharides.…”

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confidence: 99%

Plant gum identification in historic artworks

Granzotto

Arslanoglu

Rolando

et al. 2017

Sci Rep

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We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage. Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis. We developed an enzyme cocktail suitable for plant gums of unknown composition. Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified. A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles. Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including ‘Colour Box Charles Roberson & Co’ dating 1870s and drawings from the American painter Arthur Dove (1880–1946). This is the first time that a gum is accurately identified in a cultural heritage sample using structural information. Furthermore, this methodology is applicable to other domains (food, cosmetic, pharmaceutical, biomedical).

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confidence: 99%

Plant gum identification in historic artworks

Granzotto

Arslanoglu

Rolando

et al. 2017

Sci Rep

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“…On the other hand, chemical modifications occurring to a variable extent in paint layers upon ageing [11–14], can still impair positive protein identification [36]. In other words, if the detection of specific peptides univocally identifies a protein in a sample, the non-identification of specific peptides does not necessarily rule out the presence of a protein.…”

Section: Resultsmentioning

confidence: 99%

GC/MS and proteomics to unravel the painting history of the lost Giant Buddhas of Bāmiyān (Afghanistan)

et al. 2017

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A chemical investigation of the organic paint binders of the Giant Buddhas of Bāmiyān was performed using an analytical approach based on mass spectrometry, combining traditional gas chromatography/mass spectrometry protocols with advanced proteomics methodologies. The research was carried out on a selection of rescued fragments. The data revealed the use of egg proteins as the paint binders of the original layers, in accordance with the traditional use of this proteinaceous medium in antiquity, spanning from the Mediterranean basin to the Far East, and already in the Bronze Age. Egg tempera was thus known to artists of the region in the first centuries AD, probably also due to the position of the Bāmiyān valley, which was connected to the Silk Road. Milk was found in the first historical overpaintings. A new proteomics approach was used, which was able to identify the source of the milk proteins present in the restoration layers, despite their age and degradation. In particular cow’s and goat's milk were both found, in agreement with the documented presence of rich pastures in the Bāmiyān valley when the historical restorations were carried out. Investigating the materials of the Giant Buddhas not only enabled us to obtain isolated data on these invaluable works of art, which are now lost, but contributes to understanding the big “puzzle” of our past and the development of our culture, by implementing and supporting written sources, stylistic and anthropological studies with molecular data.

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“…The EVA technology has the unique property of leaving intact the items under analysis, yet collecting enough surface material for permitting subsequent instrumental analysis such as GC‐MS or high‐resolution proteomics with advanced mass spectrometers. There are several other techniques for exploring surfaces, such as UV/Vis, fluorescence analysis, IR, and other types of spectroscopies, which however do not permit collection of surface material for chemical analyses . When this is performed, one has to sacrifice (even if minimally) a portion of the item being scrutinized, thus permanently damaging our cultural heritage.…”

Section: Discussionmentioning

confidence: 99%

Anton Chekhov and Robert Koch Cheek to Cheek: A Proteomic Study

D’Amato

Zilberstein

et al. 2018

Proteomics

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Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in the State Literary-Memorial Museum-Reserve A. P. Chekhov Melikhovo (nearby Moscow), have been analyzed by applying EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C resins) diskettes to these surfaces. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectrometry. The environmental microbiota present on samples and the Mycobacterium tuberculosis strain were described by a meta-proteomics approach. Eight identified M. tuberculosis proteins confirmed the presence of the bacterium and the cause of Chekhov's death, in addition to several sequenced peptides belonging to other bacterial species. The human plasma proteins and human keratins, detected on a tiny blood spot on the shirt, demonstrated the power of the combined approach.

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Proteins in Art, Archaeology, and Paleontology: From Detection to Identification

Cited by 141 publications

References 607 publications

Plant gum identification in historic artworks

Plant gum identification in historic artworks

GC/MS and proteomics to unravel the painting history of the lost Giant Buddhas of Bāmiyān (Afghanistan)

Anton Chekhov and Robert Koch Cheek to Cheek: A Proteomic Study

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