Proteins in the Subretinal Fluid

Abstract

This study was designed to improve the understanding of the mechanisms that govern the origin of retinal detachment (RD) by examining the quantitative composition of proteins in the subretinal fluid (SRF). Inflammation proteins and immunoglobulins (Ig) were titrated from SRF and sera in 25 patients with RD The following concentrations were found in SRF: total proteins 16 g/l, albumin 12.6 g/l; pre-albumin 0.37 g/l; transferin 1.8 g/l in 68% of cases; haptoglobin and alpha 2 macroglobin were present in only one SRF: alpha 1 antitrypsin 0.86 g/l in 48%; orosomucoid 0.57 g/l in 32%; IgG 2 g/l in 52%; IgA 0.17 g/l in 88% and IgM 15.9 mg/l in 56%. Correlations were found between the duration of detachment and the concentration of total proteins (p < 0.01). The extent of RD was correlated with the concentration of IgG or IgA in the SRF (p < 0.01). These findings suggest that the correlation between the Ig concentration and the extent of RD is an argument for the participation of choriocapillaris in the genesis of SRF.

Blood Components and OCT Reflectivity Evaluated in Animal Model

“…5 The major protein in the SRF was found to be albumin. 19 These findings are compatible with the present results. However, the OCT reflectivity of SRF was reported to be high in diabetic macular edema (DME).…”

Concentration of neuron-specific enolase and S100 protein in the subretinal fluid of rhegmatogenous retinal detachment

“…S100 concentration in SRF therefore increases proportionally to the protein concentration. It is worth noting that, according to Berrod et al, protein concentration in SRF is linked to the duration of RRD [2]. No correlation could be established between NSE or S100 and the anatomical or functional prognosis because retinal cryoapplications strongly increase the concentration of these two markers.…”

“…The composition of subretinal fluid (SRF) varies according to the duration of the rhegmatogenous retinal detachment (RRD) [2,5]. SRF is not merely a transudate or an exudate, and its origin can be retinal, vitreous or seric.…”

Differential Optical Density of Subretinal Spaces

“…The most abundant protein is albumin, with the protein concentration varying as a function of the duration of the RD. 10 SRF lipid concentration in RRD varies from 0.1 to 2.4 mg/mL, 11 and the main lipid components of SRF on chromatography are phospholipids, esterified cholesterol, and triglycerides. There are very few fatty acids.…”