Proteins of rat serum IV. Time‐course of acute‐phase protein expression and its modulation by indomethacine

Eberini, Ivano; Miller, Ingrid; Zancan, Valeria; Bolego, Chiara; Puglisi, L.; Gemeiner, Manfred; Gianazza, Elisabetta

doi:10.1002/9783527613489.ch34

Cited by 3 publications

(5 citation statements)

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“…Other changes have been reported associated with malnutrition (45), haptoglobin in Duchenne muscular dystrophy (46), haptoglobin in Down syndrome (47), apoA-I during parturition (48), return from extended space flight (49) (possibly an acute phase response to resuming 1 ϫ g), apoA-I isoforms in heart disease (50), human chorionic gonadotropin isoforms in patients with trophoblastic tumors (51), apoE isoforms in chronic spinal pain (52), and oxidized plasma proteins in Alzheimer's disease (53). Important parallel work on the rat plasma proteome and its perturbation by experimental treatments (not reviewed in detail here) have been carried out by Gianazza and colleagues (54,55).…”

Section: Two-dimensional Electrophoresismentioning

confidence: 99%

The Human Plasma Proteome

Anderson¹,

Anderson²

2002

Molecular & Cellular Proteomics

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1,585

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The human plasma proteome holds the promise of a revolution in disease diagnosis and therapeutic monitoring provided that major challenges in proteomics and related disciplines can be addressed. Plasma is not only the primary clinical specimen but also represents the largest and deepest version of the human proteome present in any sample: in addition to the classical "plasma proteins," it contains all tissue proteins (as leakage markers) plus very numerous distinct immunoglobulin sequences, and it has an extraordinary dynamic range in that more than 10 orders of magnitude in concentration separate albumin and the rarest proteins now measured clinically. Although the restricted dynamic range of conventional proteomic technology (two-dimensional gels and mass spectrometry) has limited its contribution to the list of 289 proteins (tabulated here) that have been reported in plasma to date, very recent advances in multidimensional survey techniques promise at least double this number in the near future. Abundant scientific evidence, from proteomics and other disciplines, suggests that among these are proteins whose abundances and structures change in ways indicative of many, if not most, human diseases. Nevertheless, only a handful of proteins are currently used in routine clinical diagnosis, and the rate of introduction of new protein tests approved by the United States Food and Drug Administration (FDA) has paradoxically declined over the last decade to less than one new protein diagnostic marker per year. We speculate on the reasons behind this large discrepancy between the expectations arising from proteomics and the realities of clinical diagnostics and suggest approaches by which protein-disease associations may be more effectively translated into diagnostic tools in the future.

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Section: Two-dimensional Electrophoresismentioning

confidence: 99%

The Human Plasma Proteome

Anderson¹,

Anderson²

2002

Molecular & Cellular Proteomics

3,887

1,585

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“…Studies by Gianazza et al on rat serum identified 34 proteins with human homologues and concluded that even abundant proteins could be markers for disease states [30][31][32]. Traditionally, it was believed that the only source of Immunoglobulins (IgGs) was mature B lymphocytes, but recently, some researchers have reported that IgG could also be detected in carcinoma cells derived from epithelium [33].…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of differentially expressed serum proteins in lung cancer

et al. 2012

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AbstractLung cancer continues to represent a major public health concern with high morbidity and mortality worldwide. Early detection of lung cancer is problematic due to a lack of diagnostic markers with high sensitivity and specificity. To determine the differently expressed proteins in the serum of lung cancer and identify the function of such proteins, two-dimensional electrophoresis (2DE) and liquid chromatography mass spectrometry (LC-MS) were used to screen the serum of lung cancer model induced by 4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanone (NNK). A total of 25 protein spots were qualitatively different and 6 were quantitatively different in the serum from rats bearing induced lung cancer when compared with normal controls. Two of the proteins that showed major changes in concentration in sera were identified to be Immunoglobulin γ 2A chain C region (heavy chain) and Transferrin by LC-MS/MS.

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“…Analysis of rat serum by 2-DE thus provides a very sensitive way to monitor onset and progression of any pathological state involving an inflammatory component as well as to assess the outcome of any therapeutic intervention. We could validate such principles in a few model systems (various types of experimentallyinduced inflammation [5][6][7], a genetically-determined susceptibility to cerebro-vascular disease [8][9][10][11]). We could then apply similar concepts to the analysis of cerebrospinal fluid (CSF) proteome [12].…”

Section: Anniversariesmentioning

confidence: 97%

“…We then used these models for the assessment of the effects of non-steroidal anti-inflammatory drugs (NSAID), namely indomethacin [6,7] and ibuprofen [6]. In the arthritis model, both drugs inhibited the effects of the inflammatory stimulus but to a different extent and by affecting different proteins.…”

mentioning

confidence: 99%

“…The first topic we wish to review in this chapter is the characterization of a few models of acute and sub-chronic inflammation, because they provide a description of the features of the acute phase reaction in the rat and may be regarded as a background and a reference for correlations with any pathological status. We have used ourselves the model based on the intramuscular injection of turpentine -a mixture of monoterpene hydrocarbons obtained from various Pinus species -to derive the time-course data and the influence of gender on the expression of the various APR [4,5,7]. We also resorted to the adjuvant arthritis model [6], a T cell-mediated autoimmune condition induced in the susceptible strains (in our case, female Lewis rats) by injecting a suspension of heat-killed Mycobacterium tuberculosis in paraffin oil.…”

mentioning

confidence: 99%

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