Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting

Abstract

In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.

Functional Analysis of the Glucuronyltransferases GlcAT-P and GlcAT-S of Drosophila melanogaster: Distinct Activities towards the O-linked T-antigen

“…It has already been reported that molecular chaperones in Drosophila can be secreted into the extracellular space [ 12 ]. ER-localized heat-shock proteins, disulfide isomerases and inositol phosphatases have previously been reported as O -glycosylated in “simple cells” (S2-KO cell models without the ability of core-GalNAc elongation) and proteins from the heat-shock family 70 have already been identified as O -glycoproteins in Drosophila [ 13 , 14 , 15 ].…”

Environmental proteomics, biodiversity statistics and food-web structure

“…There are approximately 6,000–60,000 protein-encoding genes in a single prokaryote or eukaryote species‘ genome [36], and even a single tissue sampled from a single multicellular species contains hundreds or even thousands of distinct proteins [37]. In ecological assemblages that contain both microbes and macrobes, proteomic diversity likely will exceed 10 10 expressed proteins because— unlike the — genome the proteome of a multicellular organism is not constant during its lifetime, but changes ontogenetically [38] and in response to changing biotic and abiotic conditions [9]. …”

Molecular Social Interactions

“…Of the more than 180 predicted extracellular proteins present in the reproductive secretory glands of male D. melanogaster , over 100 have been confirmed to be transferred to the female along with sperm (e.g., reviewed in Ravi Ram & Wolfner 2007a and Chapman 2008; see also Walker et al 2006; Chintapalli et al 2007; Findlay et al 2008, 2009; Takemori & Yamamoto 2009). Many of the transferred proteins fall into conserved protein classes found in the seminal fluid of most animals studied to date and include proteases, protease inhibitors, acid lipases, cysteine rich secretory proteins (CRISPs), and lectins (Mueller et al 2004; Ravi Ram & Wolfner 2007a).…”