Proteoform profiling of peripheral blood serum proteins from pregnant women provides a molecular IUGR signature

Wölter, Manja; Röwer, Claudia; Koy, Cornelia; Rath, Werner; Pecks, Ulrich; Glocker, Michael O.

doi:10.1016/j.jprot.2016.04.027

Cited by 26 publications

(53 citation statements)

References 53 publications

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“…A quick access to the most important publications in the field and an overview of the history and status quo in proteomics may be taken from current reviews with focus on 2D gel‐based proteome analysis and references therein, and a few application examples shall be mentioned. Analysis of protein compositions in biological samples of high complexity has been successfully performed using combinations of electrophoresis methods, such as SDS‐PAGE and 2DE, with MS and provided detailed molecular information on tissues and on protein‐containing biological fluids , respectively. Proteome analyses have been applied by us and others to discover expression differences , and/or to reveal mechanisms of molecular or dynamic processes of protein expression in cells under exposure of external stimuli .…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS‐PAGE and 2D gel electrophoresis

et al. 2019

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Highly homogenous α zein protein was isolated from maize kernels in an environment‐friendly process using 95% ethanol as solvent. Due to the polyploidy and genetic polymorphism of the plant source, the application of high resolution separation methods in conjunction with precise analytical methods, such as MALDI‐TOF‐MS, is required to accurately estimate homogeneity of products that contain natural zein protein. The α zein protein product revealed two main bands in SDS‐PAGE analysis, one at 25 kDa and other at 20 kDa apparent molecular mass. Yet, high resolution 2DE revealed approximately five protein spot groups in each row, the first at ca. 25 kDa and the second at ca. 20 kDa. Peptide mass fingerprinting data of the proteins in the two dominant SDS‐PAGE bands matched to 30 amino acid sequence entries out of 102 non‐redundant data base entries. MALDI‐TOF‐MS peptide mapping of the proteins from all spots indicated the presence of only α zein proteins. The most prominent ion signals in the MALDI mass spectra of the protein mixture of the 25 kDa SDS gel band after in‐gel digestion were found at m/z 1272.6 and m/z 2009.1, and the most prominent ion signals of the protein mixture of the 20 kDa band after in‐gel digestion were recorded at m/z 1083.5 and m/z 1691.8. These ion signals have been found typical for α zein proteins and may serve as marker ion signals which upon chymotryptic digestion reliably indicate the presence of α zein protein in two hybrid corn products.

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Section: Introductionmentioning

confidence: 99%

Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS‐PAGE and 2D gel electrophoresis

et al. 2019

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“…The study cohort described here encompassed serum samples from pregnant women that previously were investigated in both our primary study on fetal cord blood proteome profiling and our recently published study of maternal peripheral blood apolipoprotein CII and CIII proteoform abundances . In both studies, differentiation of serum samples into IUGR and CTRL was achieved with great confidence for which reason this well‐studied cohort represented an excellent sample set for the assessment of novel discriminatory methods performed here that provide great potential for future clinical application.…”

Section: Discussionmentioning

confidence: 99%

“…Blood samples (up to 9 mL, each) were taken from each individual antenatally from the right or left cubital vein using monovette syringes (Serum Z/9 mL; Monovette, Sarstedt, Germany). Serum was prepared in the clinic as described . Altogether, time between blood sample collection and storage of frozen serum aliquots averaged around less than 1 h. Frozen serum aliquots were shipped on dry ice to Proteome Center Rostock (for more information see Experimental Section, Supporting Information).…”

Section: Methodsmentioning

confidence: 99%

“…We identified specific marker proteins that are known to be involved in the pathomechanisms of atherosclerosis by the regulation of triglyceride levels . Particularly pro‐apolipoprotein CII and apolipoprotein CIII together with its distinctly differently glycosylated isoforms enabled us to discriminate pregnancies with IUGR from control individuals ahead of birth, and independent from other biometrical data …”

Section: Introductionmentioning

confidence: 99%

“…In an effort to potentially widen the spectrum of future screening biomarkers for detection of pregnancy complications, such as IUGR, we conducted a retrospective study with the same 30 maternal serum samples that we had investigated previously . With IUGR in focus and our previous study results in mind, we focused our interest to evaluate levels of further apolipoproteins in peripheral blood of pregnant women.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Maternal Apolipoprotein B100 Serum Levels are Diminished in Pregnancies with Intrauterine Growth Restriction and Differentiate from Controls

Wölter

Okai

Smith

et al. 2018

Proteomics Clinical Apps

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Human cord plasma proteomic analysis reveals sexually dimorphic proteins associated with intrauterine growth restriction

Akinyemi,

Du,

Aguilan

et al. 2023

Proteomics

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Intrauterine growth restriction (IUGR) is associated with increased risk of cardiometabolic disease later in life and has been shown to affect female and male offspring differently, but the mechanisms remain unclear. The purpose of this study was to identify proteomic differences and metabolic risk markers in IUGR male and female neonates when compared to appropriate for gestational age (AGA) babies that will provide a better understanding of IUGR pathogenesis and its associated risks. Our results revealed alterations in IUGR cord plasma proteomes with most of the differentially abundant proteins implicated in peroxisome pathways. This effect was evident in females but not in males. Furthermore, we observed that catalase activity, a peroxisomal enzyme, was significantly increased in females (p < 0.05) but unchanged in males. Finally, we identified risk proteins associated with obesity, type‐2 diabetes, and glucose intolerance such as EGF containing fibulin extracellular matrix protein 1 (EFEMP1), proprotein convertase subtilisin/kexin type 9 (PCSK9) and transforming growth factor beta receptor 3 (TGFBR3) proteins unique to females while coagulation factor IX (C9) and retinol binding protein 4 (RBP4) are unique in males. In conclusion, IUGR may display sexual dimorphism which may be associated with differences in lifelong risk for cardiometabolic disease between males and females.

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Proteoform profiling of peripheral blood serum proteins from pregnant women provides a molecular IUGR signature

Cited by 26 publications

References 53 publications

Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS‐PAGE and 2D gel electrophoresis

Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS‐PAGE and 2D gel electrophoresis

Maternal Apolipoprotein B100 Serum Levels are Diminished in Pregnancies with Intrauterine Growth Restriction and Differentiate from Controls

Human cord plasma proteomic analysis reveals sexually dimorphic proteins associated with intrauterine growth restriction

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