Proteogenomic Analyses Revealed Favorable Metabolism Pattern Alterations in Rotifer Brachionus plicatilis Fed with Selenium-rich Chlorella

Abstract

Organoselenium have garnered attention because of their potential to be used as ingredients in new anti-aging and antioxidation medicines and food. Rotifers are frequently used as a model organism for aging research. In this study, we used Se-enriched Chlorella (Se- Chlorella), a novel organoselenium compound, to feed Brachionus plicatilis to establish a rotifer model with a prolonged lifespan. The results showed that the antioxidative effect in Se-enriched rotifer was associated with an increase in guaiacol peroxidase (GPX) and catalase (CAT). The authors then performed the first proteogenomic analysis of rotifers to understand their possible metabolic mechanisms. With the de novo assembly of RNA-Seq reads as the reference, we mapped the proteomic output generated by iTRAQ-based mass spectrometry. We found that the differentially expressed proteins were primarily involved in antireactive oxygen species (ROS) and antilipid peroxidation (LPO), selenocompound metabolism, glycolysis, and amino acid metabolisms. Furthermore, the ROS level of rotifers was diminished after Se- Chlorella feeding, indicating that Se- Chlorella could help rotifers to enhance their amino acid metabolism and shift the energy generating metabolism from tricarboxylic acid cycle to glycolysis, which leads to reduced ROS production. This is the first report to demonstrate the anti-aging effect of Se- Chlorella on rotifers and to provide a possible mechanism for this activity. Thus, Se- Chlorella is a promising novel organoselenium compound with the potential to prolong human lifespans.

Effects of selenium supplementation on the antioxidation response in the rotifer ( Brachionus plicatilis )

“…Se-enriched Chlorella pyrenoidesa was developed as an antioxidant feed for aquaculture (Zhong & Cheng, 2017). Also, the previous study verified the effect and the mechanism of Se-enriched C. pyrenoidesa treatments on rotifers lifespan (Sun et al, 2018).…”

“…The rotifers, Brachionus plicatilis (90% of L type), were obtained from the collection of the Institute of Hydrobiology, Jinan University. After hatching, the rotifers were kept in saliferous (10‰) EPA media at 25°C (Sun et al., 2018). A clonal culture of non‐Se‐fortified and Se‐fortified green alga, C. pyrenoides,a was established at 3 × 10 6 cells per ml (Sun et al., 2018).…”

“…After hatching, the rotifers were kept in saliferous (10‰) EPA media at 25°C (Sun et al., 2018). A clonal culture of non‐Se‐fortified and Se‐fortified green alga, C. pyrenoides,a was established at 3 × 10 6 cells per ml (Sun et al., 2018). The algae were cultured in BG11 media at 25°C in 3‐L flasks under constant fluorescent illumination of 4,000 lux (Sun et al., 2014).…”

“…The total Se content in rotifers was determined by inductively coupled plasma-mass spectrometry (ICP-MS, Agilent 7700ce; Agilent Technologies) as described previously (Sun et al, 2018).…”

Versatile Dibenzothio[seleno]phenes via Hexadehydro-Diels–Alder Domino Cyclization

“…In recent years, the use of group-16 elements such as sulfur and selenium in biological processes, materials science, and anti-cancer drugs has increasingly attracted interest among researchers (Chen et al, 2013; Sun et al, 2018; Waldvogel et al, 2018). During the manufacturing of perfumes, dyes, polymers, medicine materials, and pharmaceuticals, thiophene and selenophene could be significant intermediates (Duffield-Lillico et al, 2002; Sancineto et al, 2015).…”