Proteoglycans of reactive rat cortical astrocyte cultures: Abundance of N-unsubstituted glucosamine-enriched heparan sulfate

Hering, Thomas M.; Beller, Justin; Calulot, Christopher M.; Centers, Adrian; Snow, Diane

doi:10.1016/j.matbio.2014.11.006

Cited by 5 publications

(4 citation statements)

References 67 publications

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“…In particular, the ability of heparan sulfate domains to mediate binding of specific proteins is fundamental to the organization of protein–receptor interactions at the cell surface and for the creation of chemotactic gradients of growth factors and chemokines . For example, heparan sulfate facilitates the interaction of growth factors, such as FGF‐2, VEGF and heparin‐binding epidermal‐like growth factor, with their respective receptors . Upon EC injury resynthesis of membrane bound glycosaminoglycans occurs where sulfation patterns, and consequently also growth factor and morphogen binding, are modified to adapt to the altered microenvironment (for review see Rabelink et al ).…”

Section: The Perivascular Extracellular Matrix As a Signaling Platformentioning

confidence: 99%

Concise Review: The Endothelial Cell Extracellular Matrix Regulates Tissue Homeostasis and Repair

Witjas

Berg

et al. 2018

Stem Cells Translational Medicine

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All tissues are surrounded by a mixture of noncellular matrix components, that not only provide physical and mechanical support to cells, but also mediate biochemical signaling between cells. The extracellular matrix (ECM) of endothelial cells, also known as the perivascular matrix, forms an organ specific vascular niche that orchestrates mechano‐, growth factor, and angiocrine signaling required for tissue homeostasis and organ repair. This concise review describes how this perivascular ECM functions as a signaling platform and how this knowledge can impact the field of regenerative medicine, for example, when designing artificial matrices or using decellularized scaffolds from organs. Stem Cells Translational Medicine 2019;8:375–382

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Section: The Perivascular Extracellular Matrix As a Signaling Platformentioning

confidence: 99%

Concise Review: The Endothelial Cell Extracellular Matrix Regulates Tissue Homeostasis and Repair

Witjas

Berg

et al. 2018

Stem Cells Translational Medicine

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“…Rat cortical astrocytes were shown to produce more HS than CS in culture with a relatively higher content of unsubstituted glucosamine disaccharides. The glycosaminoglycan chains of higher negative charge were more effective at stimulating nerve growth factor (NGF) signaling in PC12 cells but the protein core to which the HS was attached was not investigated [152]. The heparin binding domain of laminin is responsible for neurite outgrowth together with NGF [127] implicating HS proteoglycans in this process.…”

Section: Glial Scarring In Neural Tissuesmentioning

confidence: 99%

The multifaceted roles of perlecan in fibrosis

et al. 2018

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Perlecan, or heparan sulfate proteoglycan 2 (HSPG2), is a ubiquitous heparan sulfate proteoglycan that has major roles in tissue and organ development and wound healing by orchestrating the binding and signaling of mitogens and morphogens to cells in a temporal and dynamic fashion. In this review, its roles in fibrosis are reviewed by drawing upon evidence from tissue and organ systems that undergo fibrosis as a result of an uncontrolled response to either inflammation or traumatic cellular injury leading to an over production of a collagen-rich extracellular matrix. This review focuses on examples of fibrosis that occurs in lung, liver, kidney, skin, kidney, neural tissues and blood vessels and its link to the expression of perlecan in that particular organ system.

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“…The neurite outgrowth assays were performed as described in detail previously [29,33]. Briefly, collagen-coated 96-well plates were incubated with varying concentrations of aggrecan in 0.1 M phosphate buffered saline (PBS) overnight at 4 • C. Following adsorption of aggrecan, wells were left untreated or were treated with enzymes as described above.…”

Section: Neurite Outgrowth Assaymentioning

confidence: 99%

Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan

Hering

Beller

Calulot

et al. 2020

Biology

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The role of proteoglycans in the central nervous system (CNS) is a rapidly evolving field and has major implications in the field of CNS injury. Chondroitin sulfate proteoglycans (CSPGs) increase in abundance following damage to the spinal cord and inhibit neurite outgrowth. Major advances in understanding the interaction between outgrowing neurites and CSPGs has created a need for more robust and quantitative analyses to further our understanding of this interaction. We report the use of a high-throughput assay to determine the effect of various post-translational modifications of aggrecan upon neurite outgrowth from NS-1 cells (a PC12 cell line derivative). Aggrecan contains chondroitin sulfate, keratan sulfate, and N-linked oligosaccharides (N-glycans), each susceptible to removal through different enzymatic digestions. Using a sequential digestion approach, we found that chondroitin sulfate and N-glycans, but not keratan sulfate, contribute to inhibition of neurite outgrowth by substrate-bound aggrecan. For the first time, we have shown that N-linked oligosaccharides on aggrecan contribute to its inhibition of neuritogenesis.

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Proteoglycans of reactive rat cortical astrocyte cultures: Abundance of N-unsubstituted glucosamine-enriched heparan sulfate

Cited by 5 publications

References 67 publications

Concise Review: The Endothelial Cell Extracellular Matrix Regulates Tissue Homeostasis and Repair

Concise Review: The Endothelial Cell Extracellular Matrix Regulates Tissue Homeostasis and Repair

The multifaceted roles of perlecan in fibrosis

Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan

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