Proteolysis-mediated protection of gold nanoparticles for sensitive activity assay of peptidases

Le, Dinh-Vu; Nguyen, Van-Trong; Tang, Li‐Juan; Jiang, Jian‐Hui; Yu, Ru‐Qin; Wang, Yuzhi

doi:10.1016/j.talanta.2013.01.016

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…For example, DNA conjugated AuNPs play an important role in biosensing and in nanobiotechnology, and they find many applications in molecular diagnostic [4]–[7], nanofabrication [8], molecular nanoelectronic [9], cell imaging and gene regulation [10]–[13]. Concerning this last possible use, nanoparticles based probes play an important role not only in enhancing imaging sensitivity and resolution but also in possessing “molecular imaging” capability [14]–[15]. One of the advantages of nanoparticle probes is their flexibility when conjugated with DNA, peptides, and antibodies for monitoring specific molecular events such as gene expression and other biological processes as cell metastasis.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Light Scattering on Bioconjugated Laser Generated Gold Nanoparticles

et al. 2014

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Gold nanoparticles (AuNPs) conjugated to DNA are widely used for biomedical targeting and sensing applications. DNA functionalization is easily reached on laser generated gold nanoparticles because of their unique surface chemistry, not reproducible by other methods. In this context, we present an extensive investigation concerning the attachment of DNA to the surface of laser generated nanoparticles using Dynamic Light Scattering and UV-Vis spectroscopy. The DNA conjugation is highlighted by the increase of the hydrodynamic radius and by the UV-Vis spectra behavior. Our investigation indicates that Dynamic Light Scattering is a suitable analytical tool to evidence, directly and qualitatively, the binding between a DNA molecule and a gold nanoparticle, therefore it is ideal to monitor changes in the conjugation process when experimental conditions are varied.

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Section: Introductionmentioning

confidence: 99%

Dynamic Light Scattering on Bioconjugated Laser Generated Gold Nanoparticles

et al. 2014

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“…Guarise and colleagues 96 first demonstrated a simple enzyme-inhibited AuNP aggregation for colorimetric protease assay by cleaving the peptide substrates modified on the AuNP surface. In similar fashion, many attempts have been made to analyze various cancer-related enzymatic activities, including methyltransferase, 97 protein kinase C, 98 adenosine deaminase, 99 alkaline phosphatase (AP), 100 MMP, 101 peptidases, 102 telomerase, 103−105 as well as hyaluronidase. 106 For instance, Wang et al 103 described a no-wash colorimetric method for visual analysis of human telomerase activity using primer-modified AuNPs, where the presence of target could lead to the elongation of primer preconjugated on the surface of AuNPs that can fold into a G-quadruplex to inhibit AuNP aggregation (Figure 3A).…”

Section: Target-induced Nanoparticle Dispersionmentioning

confidence: 99%

“…Guarise and colleagues first demonstrated a simple enzyme-inhibited AuNP aggregation for colorimetric protease assay by cleaving the peptide substrates modified on the AuNP surface. In similar fashion, many attempts have been made to analyze various cancer-related enzymatic activities, including methyltransferase, protein kinase C, adenosine deaminase, alkaline phosphatase (AP), MMP, peptidases, telomerase, − as well as hyaluronidase . For instance, Wang et al .…”

Section: Target-induced Nanoparticle Dispersionmentioning

confidence: 99%

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

et al. 2017

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In vitro biosensors have been an integral component for early diagnosis of cancer in the clinic. Among them, no-wash biosensors, which only depend on the simple mixing of the signal generating probes and the sample solution without additional washing and separation steps, have been found to be particularly attractive. The outstanding advantages of facile, convenient, and rapid response of no-wash biosensors are especially suitable for point-of-care testing (POCT). One fast-growing field of no-wash biosensor design involves the usage of nanomaterials as signal amplification carriers or direct signal generating elements. The analytical capacity of no-wash biosensors with respect to sensitivity or limit of detection, specificity, stability, and multiplexing detection capacity is largely improved because of their large surface area, excellent optical, electrical, catalytic, and magnetic properties. This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells. Representative examples are selected to demonstrate the proof-of-concept with promising applications for in vitro diagnostics of cancer. Finally, a brief discussion of common unresolved issues and a perspective outlook on the field are provided.

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“…On the basis of detection methods, homogeneous assays can be further classified into colorimetric assays, 20,21 mass spectrometry-based assays, [22][23][24] and fluorescence resonance energy transfer (FRET) assays. [25][26][27][28][29][30][31][32][33][34] More recently, nanomaterials such as noble metal nanoparticles, [35][36][37][38][39][40][41][42][43][44][45][46] quantum dots (QDs), [47][48][49][50][51][52][53][54][55][56][57][58][59] and graphene oxide (GO) [60][61][62][63][64] are also used in the homogeneous protease assays with impressive detection limits. However, homogenous assays generally have a limited multiplexed sensing capability.…”

Section: Introductionmentioning

confidence: 99%

Recent developments in protease activity assays and sensors

Ong

Yang

2017

Analyst

105

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Proteases play a pivotal role in regulating important physiological processes from food digestion to blood clotting. They are also important biomarkers for many diseases such as cancers. The importance of proteases has led to extensive efforts in the screening of proteases and their inhibitors as potential drug molecules. For example, human immunodeficiency virus (HIV) patients have been treated with HIV-1 protease inhibitors to prolong the life expectancy of patients. Such a close relationship between diseases and proteases provides a strong motivation for developing sensitive, selective, and robust protease assays and sensors, which can be exploited to discover new proteases and inhibitors. In this aspect, protease assays based on levels of proteolytic activities are more relevant than protease affinity assays such as immunoassays. In this review, recent developments of protease activity assays based on different detection principles are discussed and compared. For homogenous assays, fluorescence-based techniques are the most popular due to their high sensitivity and quantitative results. However, homogeneous assays have limited multiplex sensing capabilities. In contrast, heterogeneous assays can be employed to detect multiple proteases simultaneously, given the microarray technology that is already available. Among them, electrochemical methods, surface spectroscopy techniques, and enzyme-linked peptide protease assays are commonly used. Finally, recent developments in liquid crystal (LC)-based protease assays and their applications for detecting proteases and their inhibitors are discussed.

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Proteolysis-mediated protection of gold nanoparticles for sensitive activity assay of peptidases

Cited by 5 publications

References 30 publications

Dynamic Light Scattering on Bioconjugated Laser Generated Gold Nanoparticles

Dynamic Light Scattering on Bioconjugated Laser Generated Gold Nanoparticles

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

Recent developments in protease activity assays and sensors

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