Proteome analysis of Bemisia tabaci suggests specific targets for RNAi mediated control

Mishra, Manisha; Saurabh, Sharad; Maurya, Rashmi; Mudawal, Anubha; Parmar, Devendra; Singh, Pradhyumna Kumar

doi:10.1016/j.jprot.2015.11.020

Cited by 16 publications

(11 citation statements)

References 63 publications

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“…Therefore, proteome of plant species changes as a response to biotic “pests” [7–9] and abiotic “chemical, salt, and drought stress” [3, 10, 11]. Proteome analysis of roots and leaves revealed a synergetic responsive network under stress; roots rapidly sensed and responded to stress, after which the stress signals were transferred to leaves and both roots and leaves showed similar metabolic pathways under stress with distinct changes [12].…”

Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Rabey

Al-Malki

Abulnaja

2016

International Journal of Genomics

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Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

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Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Rabey

Al-Malki

Abulnaja

2016

International Journal of Genomics

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“…It is noteworthy that the selection of vital genes in whiteflies that can be targeted through RNAi and the exploration of anti-whitefly function in the existing battery of insecticidal proteins, along with the discovery of new insecticidal proteins, are the bottleneck in the genetic manipulation approach. A limited number of studies have been performed for the sake of specific biomolecules (including insecticidal proteins) and gene targets (for silencing) that not only have detrimental impacts on whitefly but also have the potential to control whiteflies [ 110 , 111 ]. In this section, we have summarized the studies focusing on the testing of known macromolecules (gene targets for RNA interference and insecticidal proteins), their putative mode of action, and efficacy for the control of whiteflies.…”

Section: Next-generation Strategies For the Effective Control Of Whitefliesmentioning

confidence: 99%

“…Nevertheless, the identification of whitefly specific genes with no or minimum off-target effects has been a major concern. Therefore, with the help of proteome data, a sincere effort has also been made in the search for sweetpotato-whitefly-specific nucleotide sequences [ 110 ].…”

Section: Next-generation Strategies For the Effective Control Of Whitefliesmentioning

confidence: 99%

Tiny Flies: A Mighty Pest That Threatens Agricultural Productivity—A Case for Next-Generation Control Strategies of Whiteflies

Saurabh

Mishra

Rai

et al. 2021

Insects

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Whiteflies are a group of universally occurring insects that are considered to be a serious pest in their own way for causing both direct and indirect damages to crops. A few of them serve as vectors of plant viruses that are detrimental to the crop in question and cause an actual loss in productivity. A lot of attention is focused on pest control measures under the umbrella of IPM. In this review, we attempt to summarize the existing literature on how and why whiteflies are a serious concern for agriculture and society. We reviewed why there could be a need for fresh insight into the ways and means with which the pest can be combated. Here, we have emphasized next-generation strategies based on macromolecules, i.e., RNA interference and genetic engineering (for the expression of anti-whitefly proteins), as these strategies possess the greatest scope for research and improvement in the future. Recent scientific efforts based on nanotechnology and genome editing, which seem to offer great potential for whitefly/crop pest control, have been discussed. Comprehensive apprehensions related to obstacles in the path of taking lab-ready technologies into the farmers’ field have also been highlighted. Although the use of RNAi, GM crops, nanotechnologies, for the control of whiteflies needs to be evaluated in the field, there is an emerging range of possible applications with promising prospects for the control of these tiny flies that are mighty pests.

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“…In addition, the begomovirus–host plant pathosystem also includes an insect vector, which in this case is the whitefly Bemisia tabaci . Several studies have examined the proteome of B. tabaci either in the presence [ 22 ] or absence [ 23 , 24 ] of begomoviruses. Furthermore, avirulent B. tabaci was recently found to impact redox regulation, stress response, as well as lipid and carbon metabolism in susceptible pepper genotypes 2 days post feeding (dpf) [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics

Ogden

Boukari

Nava

et al. 2020

IJMS

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Tomato mottle virus (ToMoV) is a single-stranded DNA (ssDNA) begomovirus transmitted to solanaceous crops by the whitefly species complex (Bemisia tabaci), causing stunted growth, leaf mottling, and reduced yield. Using a genetic repertoire of seven genes, ToMoV pathogenesis includes the manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants (Solanum lycopersicum ‘Florida Lanai’), we generated comprehensive protein profiles of leaves subjected to feeding by either viruliferous whiteflies harboring ToMoV, or non-viruliferous whiteflies, or a no-feeding control. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of clip-cage confined whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, tomato’s response to ToMoV included proteins associated with translation initiation and elongation as well as plasmodesmata dynamics. In contrast, systemic impacts of ToMoV on younger leaves 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other antiviral plant processes. Our analysis supports previous findings and provides novel insight into tomato’s local and systemic response to whitefly feeding and ToMoV infection.

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Proteome analysis of Bemisia tabaci suggests specific targets for RNAi mediated control

Cited by 16 publications

References 63 publications

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Tiny Flies: A Mighty Pest That Threatens Agricultural Productivity—A Case for Next-Generation Control Strategies of Whiteflies

Characterization of Local and Systemic Impact of Whitefly (Bemisia tabaci) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics

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