Proteome analysis of human follicular fluid

Angelucci, Stefania; Ciavardelli, Domenico; Giuseppe, Fabrizio Di; Eleuterio, Enrica; Sulpizio, M.J.; Tiboni, Gian Mario; Giampietro, Franca; Palumbo, Paola; Ilio, Carmine Di

doi:10.1016/j.bbapap.2006.09.001

Cited by 129 publications

(131 citation statements)

References 54 publications

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“…However, a limitation of this study is that we are unable to attribute fertilization to oocyte quality only, although we Fig. 2 Spectral counts of heparan sulfate proteoglycan perlecan in the follicular fluids containing fertilized oocytes (fFF) and non-fertilized oocytes (nfFF) in each patient (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) tried to minimize the influence of sperm quality using ICSI. An increased number of samples would enable the use of a proteomics analysis to identify distinctive biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…Bayasula : A. Iwase (*) : H. Kobayashi : M. Goto : T. Nakahara : T. Nakamura : M. Kondo : Y. Nagatomo : T. Kotani : F. Kikkawa folliculogenesis and oocyte maturation. Several studies suggest that many human follicular fluid proteins derive from granulosa cells ( [1]; Jarkovska et al2010). Follicular fluid contains a variety of proteins that play important roles in follicle development and oocyte maturation and are thus thought to impact the outcome ofin vitro fertilization.Therefore,conductinganalyses of follicular fluid might be helpful in evaluating oocyte quality and identifying predictive markers for the success of IVF.…”

Section: Introductionmentioning

confidence: 99%

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A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Bayasula

Iwase

Kobayashi

et al. 2013

J Assist Reprod Genet

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Purpose Human follicular fluid constitutes the microenvironment of follicles and includes various biological active proteins that can affect follicle growth and oocyte fertilization. Conducting proteomic evaluations of human follicular fluid may be helpful for identifying potential biomarkers possibly possessing a predictive value for oocyte quality and the success of in vitro fertilization. Method We performed proteomic profiling of human follicular fluids containing oocytes that were fertilized and resulted in pregnancy and follicular fluids containing oocytes that were not fertilized in the same patients undergoing intracytoplasmic sperm injection using the LTQ Orbitrap coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses. ResultsWe identified a total of 503 proteins in human follicular fluids containing fertilized and non-fertilized oocytes obtained from 12 patients. We also found that 53 proteins exhibited significantly different spectral counts between the two groups, including heparan sulfate proteoglycan perlecan, which showed significant upregulation in the follicular fluids containing fertilized oocytes in comparison with that observed in the follicular fluids containing non-fertilized oocytes. Conclusion Our results suggest a possibility that proteins identified by LC/MS/MS in follicular fluid might not only be involved in folliculogenesis, but also function as biomarkers possessing predictive potential for oocyte maturation and the success of IVF when their expression levels are significantly different between fertilized and non-fertilized oocytes, although no distinctive biomarkers were identified in the current study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Bayasula

Iwase

Kobayashi

et al. 2013

J Assist Reprod Genet

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“…The main source of FF transferrin is from the blood circulation, and the concentration of transferrin in FF is usually higher than that in serum [39] because transferrin is also locally synthesized by granulosa cells [40,41]. Transferrin was found to inhibit progesterone production and FSHstimulated aromatase activity of granulose cells [42,43].…”

Section: Discussionmentioning

confidence: 99%

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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Purpose To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. Methods Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes. Protein expression profiles in each FF sample were analyzed respectively using twodimensional gel electrophoresis. Differentially expressed proteins were identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and validated by western blotting. Differentially expressed proteins were further analyzed using Ingenuity Pathway Analysis (IPA) software.Results Two proteins were downregulated and 11 proteins were upregulated (change ≥1.5-fold, P<0.05) in the COH group. We identified one down-egulated and seven upregulated proteins using MALDI-TOF MS. Four differentially expressed proteins, including transferrin, complement component C3 (C3), haptoglobin and alpha-1-antitrypsin (AAT), were further validated by rate nephelometry and western blotting analyses. The IPA analysis revealed a significant network involved in the humoral immune and inflammatory responses. Conclusions The eight differentially expressed proteins were related to immune and inflammatory responses in the ovary. Our results provide new insights into the influence of COH on follicular (spp) development and IVF outcomes.

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“…The ionic composition (5)(6)(7)(8), the presence of glucose, lactate, urea and other metabolites (2,(4)(5)(6)(7)(8)(9) and lipids (5,7,8) have also been determined. Some protein analyses have been also reported (8,(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

NMR metabolic profile of human follicular fluid

et al. 2010

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The environment of the oocyte during its in vivo maturation consists of follicular fluid (FF) and is surrounded by granulosa cells. The FF is derived from the sanguineous plasma and secretions, synthesised in the follicle wall, that contain a large variety of growth factors, cytokines, amino acids, and other metabolites. These metabolites are presumably involved in the physiology of the oocyte. The identification, quantification and study of FF metabolites can provide additional information about the oocyte state which can be helpful in distinguishing those oocytes that have a greater capacity to be fertilised and to develop properly. The aim of this work is to identify the metabolic profile of FF samples exhaustively using High Resolution Nuclear Magnetic Resonance (NMR). A total of 30 FF samples from oocyte donors (<35 years) were analysed. Different monodimensional (1D) and bidimensional (2D) (homo and heteronuclear) NMR experiments were acquired. A total of 131 chemical shifts were assigned and 42 metabolites, including as example glucose, lactate, acetate, acetoacetate, pyruvate and b-hydroxybutyrate, were identified. High correlations were found between these important intermediaries of the energetic metabolic pathways of the follicle which can indicate the importance of these pathways in oocyte development. Some of these identified metabolites might be useful as biomarkers of the follicular maturation state, allowing oocytes with a higher fertilisation potential to be selected, thereby increasing pregnancy rates in women following in vitro fertilisation (IVF) treatments.

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Proteome analysis of human follicular fluid

Cited by 129 publications

References 54 publications

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

A proteomic analysis of human follicular fluid: comparison between fertilized oocytes and non-fertilized oocytes in the same patient

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

NMR metabolic profile of human follicular fluid

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