Proteome Analysis of Potato under Salt Stress

Abstract

Because salt stress is a major abiotic source of stress on potato crops, the molecular mechanism of the response of potato plants to salt stress was examined. On exposure to salt, the salt-sensitive cultivar Concord showed a greater reduction in shoot and root length than did the salt-tolerant cultivar Kennebec. For both cultivars, the reduction in the length of shoots was more severe than that of the roots. Salt exposure increased the content of free proline and total soluble sugars in shoots of Kennebec; these remained unchanged in Concord. Proteins extracted from shoots of both cultivars exposed to 90 mM NaCl were separated by two-dimensional polyacrylamide gel electrophoresis: 322 and 305 proteins were detected in shoots of Kennebec and Concord, respectively. Of these, 47 proteins were differentially expressed under NaCl treatment in shoot of both cultivars. Among the differentially expressed proteins, photosynthesis- and protein-synthesis-related proteins were drastically down-regulated, whereas osmotine-like proteins, TSI-1 protein, heat-shock proteins, protein inhibitors, calreticulin, and five novel proteins were markedly up-regulated. These results suggest that up-regulation of defense-associated proteins may confer relative salt tolerance to potato plants.

Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity

“…Our results are in general agreement with other studies showing that both photochemical and biochemical aspects of photosynthesis are affected by salinity (Hasegawa et al, 2000;Zhu, 2003;Dubey, 2005;Aghaei et al, 2008).…”

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

“…Of these, spots 7, 21, 22 and 40 had increased abundance levels, while spots 13 and 14 had reduced abundance levels following salt stress. The upregulation of protein expression observed here has also being documented in other studies on the proteomic analysis of potato shoots [21] and rice leaf lamina [20] under salt stress. The ATP synthase complex produces ATP from ADP in the presence of a proton gradient across the thylakoid membrane [56,57], which is in turn used for various energy demanding activities during periods of stress.…”

“…Rubisco which catalyses the carbon fixation reaction in photosynthetic plants [52] has an increased abundance in all four spots (spots 16-19; Table 1) in response to salt stress. In contrast, increased degradation of Rubisco was reported in the proteomic analysis of potato under salt stress [21], sugar beet under drought stress [53], rice under chilling stress [54], and maritime pine seedlings under water stress [55]. However, in another proteomic study involving sorghum leaf under salt stress, the expression of Rubisco was not shown to be altered in response to salt stress [23].…”

“…Proteomics, the large-scale analysis of proteins from a particular organism, tissue or cell at a given time [17][18][19] has been used to study salt stress responsive protein expression in crops such as rice (Oryza sativa; [20]), potato (Solanum tuberosum; [21]) and foxtail millet (Setaria italica L. cv Prasad; [22]) amongst others. Sobhanian and co-workers reviewed some of these studies, giving a description of unique proteome changes of several other economically important food crops under salt stress [7].…”

Physiological Response and Comparative Proteomic Analysis of Tobacco Seedling Roots to NH4+

“…This indicated that ATP synthesis activity is enhanced, likely because plants need an increased energy production to deal with NH4 + toxicity. Similar observations have been reported in wheat (Wang et al, 2008b) and potato (Aghaei et al, 2008) under saline stress and Masson's pine under acid rain stress (Wang et al, 2013), indicating that sustained ATP synthesis is a common strategy for as plants under stress.…”