Proteome analysis of soybean hypocotyl and root under salt stress

Abstract

To evaluate the response of soybean to salt stress, the related changes in protein expression were investigated using the proteomic approach. Soybean plants were exposed to 0, 50, 100, and 200 mM NaCl. Especially at 200 mM, the length and fresh weight of the hypocotyl and root reduced under salt stress, while the proline content increased. Proteins from the hypocotyl and root treated with 100 mM NaCl were extracted and separated by two-dimensional polyacrylamide gel electrophoresis; 321 protein spots were detected. In response to salt stress, seven proteins were reproducibly found to be up- or down-regulated by two to sevenfold: late embryogenesis-abundant protein, beta-conglycinin, elicitor peptide three precursor, and basic/helix-loop-helix protein were up-regulated, while protease inhibitor, lectin, and stem 31-kDa glycoprotein precursor were down-regulated. These results indicate that salinity can change the expression level of some special proteins in the hypocotyl and root of soybean that may in turn play a role in the adaptation to saline conditions.

Mechanisms of Soybean Roots' Tolerances to Salinity Revealed by Proteomic and Phosphoproteomic Comparisons Between Two Cultivars

“…Our results confirmed the view that expression differences at proteomic level are involved in functional proteins, whereas differences at phosphoproteomic level are mainly related to regulatory proteins (29). Interestingly, a series of proteins related to ROS scavenging and protein folding/degradation-such as GST, APX, SOD, heat shock protein 90 -2, and Hsp70-Hsp90 organizing protein 1-were involved in salt responses of both salt-tolerant and salt-sensitive varieties, which were almost in accordance with previous studies (17,52,53). However, tolerance discriminations were possibly dominated by: (1) synthesis of flavonoid/ isoflavonoid involved in the salicylic acid defense pathway by chalcone metabolism (54,55) in Wenfeng07, compared with initiation of lateral roots by auxin response factor, auxininduced protein AUX22 and PIN6a (10) in Union85140; (2) up-regulation of ERF and MYB TFs for activating MAPK and SOS pathways to eliminate ROS and excessive salts (12,13) in Wenfeng07; and (3) regulating innate immunity via cytochrome P450 monooxygenase, chalcone isomerase, and sterol 24-C methyltransferase (56,57) specifically in Wenfeng07.…”

“…Phosphorylation of specific signaling components are known to be initiated at critical time points after plants been subjected to the salt stresses (15) and they coordinate specific metabolic processes, cell-wall porosity and lateral root initiation to help plants adapt to salt stresses (10,13,16). Recently, major high throughput strategies including transcriptomic, proteomic, and metabolomic approaches, have been used to dissect the responses of soybean root to salinity stress (17)(18)(19)(20)(21). However, most of these studies were focused on relatively late responses to salinity (e.g.…”

Proteomic analysis of seed germination under salt stress in soybeans

“…The proteomic approach, based on reproducible two-dimensional gel electrophoresis (2-DE) and powerful mass spectrometry (MS) analyses, offers the possibility of identifying those proteins (Gygi and Aebersold, 2000). In fact, the proteomics of soybean in response to abiotic stress have been studied (Zhen et al, 2007;Aghaei et al, 2008;Toorchi et al, 2009;Cheng et al, 2010;Nouri and Komatsu, 2010;Sobhanian et al, 2010). Although the majority of defense proteins are expressed in optimal growth conditions, they have been found to be either overexpressed or underexpressed during stress.…”

Morpho-Physiological Response of Stevia (Stevia Rebaudiana Bertoni) to Salinity Under Hydroponic Culture Condition (A Case Study in Iran)

“…Increased root surface is important since caused increased levels of absorption and increasing the efficiency of water and nutrients. Therefore, longer roots and more root surface can be provided possibility salinity tolerance (Aghaei et al, 2009). …”