Proteome analysis of the Atlantic salmon (Salmo salar) cell line SHK‐1 following recombinant IFN‐γ stimulation

Abstract

Type II IFN exists as a single molecule (IFN-gamma) in contrast to type I IFN, of which there are a number of different forms. IFN-gamma is involved both directly and indirectly in antiviral activity, stimulation of bactericidal activity, antigen presentation and activation of macrophages. Recently IFN-gamma was cloned from a salmonid fish, the rainbow trout and a functional recombinant protein produced exhibited IFN-gamma activity. This recombinant IFN-gamma was used to stimulate an Atlantic salmon cell line, SHK-1, to monitor the changes in protein expression by proteomic analysis 24 h after stimulation compared to unstimulated control cells. An SHK-1 cell proteome map was developed and proteins altered in abundance by the IFN-gamma stimulation were identified. Under the analytical conditions used, 22 proteins were found to be altered in abundance, 15 increased and 7 decreased. Several proteins were excised from the gel and identified, following trypsin digestion and MALDI-MS/MS/LC-MS and database interrogation. Transcriptional analysis of five mRNAs encoding proteins increased in abundance by IFN-gamma in the proteome analysis was determined by real-time PCR. We assessed the correlation between gene expression and change in abundance of proteins for these genes.

Recombinant interferon-γ activates immune responses against Edwardsiella tarda infection in the olive flounder, Paralichthys olivaceus

“…IFN-g exerts a large number of functions, allowing an infected host to respond appropriately to help eliminate a pathogen. The mechanism by which IFN-g exerts its effects appear to be highly evolutionarily conserved [28], particularly in higher vertebrates, with the classic JAK/STAT signal transduction pathway leading to induction of the expression of IFN-g-responsive genes [29].…”

Expression of interferon and interferon-induced genes in salmonids in response to virus infection, interferon-inducing compounds and vaccination

“…vig-7 and vig-8 are homologous to CXC chemokines, and vig-9 is homologous to galectin, which is described as a chemoattractant for eosinophils in mammals. Finally, the acute phase protein serum amyloid A, the chemokine g-IP10 and two proteins of unknown function, ISG12 and GIG2, have been identified as IFN-induced proteins in Atlantic salmon [27].…”

Immunomodulatory effect of arsenic on cytokine and HSP gene expression in Labeo rohita fingerlings

“…Among the numerous physiological responses that require cytokine involvement are development of cellular and humoral immune responses, induction of inflammatory response, regulation of hematopoiesis, control of cellular proliferation and differentiation [3]. There are many immunomodulatory agents like specific drugs and chemicals, certain cytokines, environmental contaminants like heavy metals and xenobiotics that modulate the immune response [4].…”