Proteome analysis of the dorsolateral prefrontal region from healthy individuals

Alexander-Kaufman, Kimberley; Dedova, Irina; Harper, Clive; Matsumoto, Izuru

doi:10.1016/j.neuint.2007.04.016

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…This fact prompted further proteomic investigations, e.g. how the grey and white matter proteomes differ from each other in normal controls [27]. …”

Section: Specific Examples Of Molecular Neuropathological Studies mentioning

confidence: 99%

The Importance of Brain Banks for Molecular Neuropathological Research: The New South Wales Tissue Resource Centre Experience

Dedova

Harding

Sheedy

et al. 2009

IJMS

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New developments in molecular neuropathology have evoked increased demands for postmortem human brain tissue. The New South Wales Tissue Resource Centre (TRC) at The University of Sydney has grown from a small tissue collection into one of the leading international brain banking facilities, which operates with best practice and quality control protocols. The focus of this tissue collection is on schizophrenia and allied disorders, alcohol use disorders and controls. This review highlights changes in TRC operational procedures dictated by modern neuroscience, and provides examples of applications of modern molecular techniques to study the neuropathogenesis of many different brain disorders.

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“…This fact prompted further proteomic investigations, e.g. how the grey and white matter proteomes differ from each other in normal controls [27]. …”

Section: Specific Examples Of Molecular Neuropathological Studies mentioning

confidence: 99%

The Importance of Brain Banks for Molecular Neuropathological Research: The New South Wales Tissue Resource Centre Experience

Dedova

Harding

Sheedy

et al. 2009

IJMS

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“…Over 500 proteins have been identified as altered by ethanol in brain tissue collected from humans and rodents: frontal cortex (Alexander-Kaufman et al, 2007a; Alexander-Kaufman et al, 2006; Etheridge et al, 2009; Lewohl et al, 2004), occipital cortex (Etheridge et al, 2009), hippocampus (Hargreaves et al, 2009; Matsuda-Matsumoto et al, 2007), corpus callosum (Kashem et al, 2008; Kashem et al, 2007), nucleus accumbens (Bell et al, 2006; McBride et al, 2009; Witzmann et al, 2003), amygdala (Bell et al, 2006), and cerebellar vermis (Alexander-Kaufman et al, 2007b). Accordingly, as presented in Table 4, the majority of the proteins detected in the current study have previously been identified as altered by ethanol exposure in human and rodent, genomic or proteomic studies.…”

Section: Discussionmentioning

confidence: 99%

“… 1 Alexander-Kaufman et al, 2006; 2 Alexander-Kaufman et al, 2007a; 3 Alexander-Kaufman et al, 2007b; 4 Bell et al, 2006; 5 Bell et al, 2009; Carr et al, 2007; 7 Ciccocioppo et al, 2006; 8 Edenberg et al, 2005; 9 Etheridge et al, 2009; 10 Flatscher-Bader et al, 2005; 11 Hargreaves et al, 2009; 12 Hill et al, 2004; 13 Hitzemann et al, 2004; 14 Hoffman & Tabakoff, 2005; 15 Iwamoto et al, 2004; 16 Kashem et al, 2007; 17 Kashem et al, 2008; 18 Kerns et al, 2005; 19 Kimpel et al, 2007; 20 Lewohl et al, 2000; 21 Lewohl et al, 2004; 22 Liu et al, 2006; 23 Masuo et al, 2009; 24 Matsuda-Matsumoto et al, 2007; 25 Mayfield et al, 2002; 26 McBride et al, 2009; 27 McBride et al, 2010; 28 Mulligan et al, 2006; 29 Prescott et al, 2006; 30 Rodd et al, 2008; 31 Saba et al, 2006; 32 Saito et al, 2004; 33 Sokolov et al, 2003; 34 Sommer et al, 2006; 35 Tabakoff et al, 2003; 36 Tabakoff et al, 2008; 37 Tabakoff et al, 2009; 38 Thibault et al, 2000; 39 Treadwell & Singh, 2004; 40 Wang et al, 2007; 41 Witzemann et al, 2003; 42 Xu et al, 2001. …”

Section: Figureunclassified

Ethanol-induced changes in the expression of proteins related to neurotransmission and metabolism in different regions of the rat brain

Zahr

Bell

Ringham

et al. 2011

Pharmacology Biochemistry and Behavior

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Despite extensive description of the damaging effects of chronic alcohol exposure on brain structure, mechanistic explanations for the observed changes are just emerging. To investigate regional brain changes in protein expression levels following chronic ethanol treatment, one rat per sibling pair of male Wistar rats was exposed to intermittent (14 hr/day) vaporized ethanol, the other to air for 26 weeks. At the end of 24 weeks of vapor exposure, the ethanol group had blood ethanol levels averaging 450 mg %, had not experienced a protracted (>16 hr) withdrawal from ethanol, and revealed only mild evidence of hepatic steatosis. Extracted brains were micro-dissected to isolate the prefrontal cortex (PFC), dorsal striatum (STR), corpus callosum genu (CCg), CC body (CCb), anterior vermis (AV), and anterior dorsal lateral cerebellum (ADLC) for protein analysis with two-dimensional gel electrophoresis. Expression levels for 54 protein spots were significantly different between the ethanol- and air- treated groups. Of these 54 proteins, tandem mass spectroscopy successfully identified 39 unique proteins, the levels of which were modified by ethanol treatment: 13 in the PFC, 7 in the STR, 2 in the CCg, 7 in the CCb, 7 in the AV, and 5 in the ADLC. The functions of the proteins altered by chronic ethanol exposure were predominately associated with neurotransmitter systems in the PFC and cell metabolism in the STR. Stress response proteins were elevated only in the PFC, AV, and ADLC perhaps supporting a role for frontocerebellar circuitry disruption in alcoholism. Of the remaining proteins, some had functions associated with cytoskeletal physiology (e.g., in the CCb) and others with transcription/translation (e.g., in the ADLC). Considered collectively, all but 4 of the 39 proteins identified in the present study have been previously identified in ethanol gene- and/or protein- expression studies lending support for their role in ethanol-related brain alterations.

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“…Given that our analyses is limited to acidic proteins this is a significant improvements when compared to other studies that looked at all proteins on a single gel and stated visualized less than 2000 proteins (i.e. spots) [4,5,[20][21][22][23][24][25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Using differential solubilization and 2‐D gel electrophoresis to visualize increased numbers of proteins in the human cortex and caudate nucleus and putamen

Dean¹,

Pavey²,

Smith³

2008

Proteomics Clinical Apps

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The aim of this study was to determine if differential solubilization of human CNS proteins would increase the total number of proteins that could be visualized using 2-D gel electrophoresis. Hence, proteins were solubilized into Tris, CHAPS and SB3-10 before separation across a pH 4-7 IEF gradient and a 12-14% SDS polyacrylamide gel, which could be achieved with a run-to-run variation of 35% in spot intensity. Because Western blot analyses suggested proteins could be in more than one detergent fraction, we completed a conservative analyses of our 2-D gels assuming spots that appeared on multiple gels at the same molecular weight and pI were the same protein.These analyses show that we had visualized over 3000 unique protein spots across three 2-D gels generated from each sample of human frontal cortex and caudate-putamen. This represented, at worst, a significant increase in the number of spots visualized in the acidic protein spectrum compared to what has been reported in other studies of human CNS. This study, therefore, supports the proposal that the analysis of the human CNS proteome using 2-D gel electrophoresis, combined with appropriate sample preparation, can be used to expand the studies on the pathologies of neurological and psychiatric diseases.

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Proteome analysis of the dorsolateral prefrontal region from healthy individuals

Cited by 7 publications

References 29 publications

The Importance of Brain Banks for Molecular Neuropathological Research: The New South Wales Tissue Resource Centre Experience

The Importance of Brain Banks for Molecular Neuropathological Research: The New South Wales Tissue Resource Centre Experience

Ethanol-induced changes in the expression of proteins related to neurotransmission and metabolism in different regions of the rat brain

Using differential solubilization and 2‐D gel electrophoresis to visualize increased numbers of proteins in the human cortex and caudate nucleus and putamen

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