2021
DOI: 10.3390/ijms22073369
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Proteome of Stored RBC Membrane and Vesicles from Heterozygous Beta Thalassemia Donors

Abstract: Genetic characteristics of blood donors may impact the storability of blood products. Despite higher basal stress, red blood cells (RBCs) from eligible donors that are heterozygous for beta-thalassemia traits (βThal+) possess a differential nitrogen-related metabolism, and cope better with storage stress compared to the control. Nevertheless, not much is known about how storage impacts the proteome of membrane and extracellular vesicles (EVs) in βThal+. For this purpose, RBC units from twelve βThal+ donors wer… Show more

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Cited by 17 publications
(36 citation statements)
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“…Network analysis was performed for the topological visualization of the statistically significant correlations between a great variety (>300 nodes) of omics (RBC membrane proteome) and physiological (e.g., cellular fragility, redox equilibrium) parameters of the βThal + and control RBC units [ 22 , 23 ], including the proteasome (components and activities). This approach helps to elucidate the possible associations between parameters of stored RBCs that may fuel future more targeted research concerning the regulation of proteasome activity, interactions, recruitment to the membrane, and release during storage.…”
Section: Resultsmentioning
confidence: 99%
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“…Network analysis was performed for the topological visualization of the statistically significant correlations between a great variety (>300 nodes) of omics (RBC membrane proteome) and physiological (e.g., cellular fragility, redox equilibrium) parameters of the βThal + and control RBC units [ 22 , 23 ], including the proteasome (components and activities). This approach helps to elucidate the possible associations between parameters of stored RBCs that may fuel future more targeted research concerning the regulation of proteasome activity, interactions, recruitment to the membrane, and release during storage.…”
Section: Resultsmentioning
confidence: 99%
“…Apart from the correlations with intracellular ROS thoroughly analyzed in this study ( Figure 2 ), the levels of proteasome activities in the βThal + cytosol were positively connected to additional redox-related variables (e.g., glutathione S-transferase, protein carbonylation; see Figure 6 and Figure 7 ). Of note, some cellular and membrane protein features of βThal + stored RBCs [ 22 , 23 ] were also present in their activity interactomes in contrast to controls: band 3, phospholipid scramblase-1, osmotic fragility ( Figure 6 ), calpain, arginase-1, RHAG, ATPase Na + /K + ( Figure 7 ). On the other hand, the control networks of cytosolic activities were dominated by calcium-related (cytosolic calcium concentration, calreticulin) and internal or membrane-associated components (e.g., day 42: positive with stomatin and myosin; negative with glycophorin A, CD44), the cytosolic concentration of Hb, and several enzymes.…”
Section: Resultsmentioning
confidence: 99%
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