Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Fernandes, César Carneiro Linhares; Rodríguez-Villamil, P.; Vasconcelos, Fábio Perdigão; Nagano, Celso Shiniti; Rossetto, R.; Moura, Arlindo de Alencar Araripe; Rondina, Davide

doi:10.1111/rda.13208

Cited by 16 publications

(22 citation statements)

References 48 publications

(61 reference statements)

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“…Therefore, it is not surprising that the α-1-antiproteinase transcript has been identified in the oviductal cells [17], presumably secreted by the oviductal epithelium. Corroborating our results, Pillai et al, [44] identified α-1-antiproteinase and other serpins (A3-1, A3-7, A3-8, D1 and H1) in bovine OF, as well as Fernandes et al, [45], in the periovulatory oviduct and uterus of goats [44], and these genes are expressed by the porcine oviduct [46]. PGK1, a non-secreted enzyme, was found in a higher concentration in the OF of pregnant heifers.…”

Section: Discussionsupporting

confidence: 90%

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid

Rodríguez‐Alonso

Maillo

Acuña

et al. 2020

IJMS

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Knowledge of how the biochemical composition of the bovine oviduct is altered due to the oviduct anatomy or the presence of an embryo is lacking. Thus, the aim of this study was to assess the effect of (I) oviduct anatomy and (II) embryo presence on oviductal fluid (OF) protein, amino acid, and carbohydrate composition. Cross-bred beef heifers (n = 19) were synchronized and those in standing estrus were randomly allocated to a cyclic (non-bred) or pregnant (artificially inseminated) group. All heifers were slaughtered on Day 3 after estrus. The oviducts ipsilateral to the corpus luteum from each animal were isolated, straightened and cut, separating ampulla and isthmus. Each portion was flushed with 500 µl of PBS enabling recovery of the oocyte/embryo. Recovered unfertilized oocytes (cyclic group) and embryos (8-cell embryos; pregnant group) were located in the isthmus of the oviduct. Samples of flushing medium from the isthmus and ampulla were used for proteomic (n = 2 per group), amino acid (n = 5), and carbohydrate (n = 5) analysis. For proteomic analysis, total protein from cyclic and pregnant samples were labelled with different cyanine fluorescent probes and separated according to the isoelectric point using immobilized pH gradient strips (pH 3-10, 17 cm, Protean ® IEF cell system, Bio Rad). Second dimension was performed in a polyacrylamide gel (12%) in the presence of SDS using a Protean II XL system (Bio Rad). Images were obtained with a Typhoon 9410 scanner and analyzed with Progenesis SameSpots software v 4.0. Amino acid content in the OF was determined by high performance liquid chromatography (HPLC). Glucose, lactate, and pyruvate were quantified using microfluorometric enzyme-linked assays. For the proteomic assessment, the results of the image analysis were compared by ANOVA. For both amino acid and carbohydrate analyses, statistical analysis was carried out by 2-way ANOVA with the Holm-Sidak nonparametric post hoc analysis. On Day 3 post-estrus, OF composition varied based on (I) anatomical region, where isthmic metabolites were present in lower (i.e., lactate, glycine, and alanine) or higher (i.e., arginine) concentrations compared to the ampulla; and (II) embryo presence, which was correlated with greater, arginine, phosphoglycerate kinase 1, serum albumin, α-1-antiproteinase and IGL@ Int.The oviduct is a tubular seromuscular organ connecting the ovary to the uterus providing a physiological environment for gamete interaction, fertilization, and early embryo development. During the first four days of embryo development, which occurs within the oviduct [1], embryonic nutritional requirements are presumed to be fulfilled largely by oviduct fluid (OF)-a complex mixture of proteins, amino acids, carbohydrates, ions, hormones, and extracellular vesicles, amongst other constituents [2]. OF composition is regulated by (I) selective transudate from the vasculature, and (II) the secretory cells within the oviduct epithelial monolayer [3]. Moreover, OF composition is influenced spatially by the anatomical...

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Section: Discussionsupporting

confidence: 90%

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid

Rodríguez‐Alonso

Maillo

Acuña

et al. 2020

IJMS

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“…However, there is evidence that metabolic perturbations encountered by the mother can alter this buffering function of the oviduct [97]. In goats, a modification of the nutritional balance in the diet altered the expression of proteins participating in apoptosis, antioxidant, and immunological activities in the periovulatory oviduct-functions important for embryo development [98].…”

Section: Metabolism and Energy Balancementioning

confidence: 99%

“…Comparison between four different diet groups identified seven differentially expressed proteins in ampullas of animals fed with 1.9 times live weight maintenance [98] Energy balance OEC gene expression Cattle…”

Section: Energy Balance Oec Proteins Goatmentioning

confidence: 99%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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The oviductal fluid is the first environment experienced by mammalian embryos at the very beginning of life. However, it has long been believed that the oviductal environment was not essential for proper embryonic development. Successful establishment of in vitro embryo production techniques (which completely bypass the oviduct) have reinforced this idea. Yet, it became evident that in vitro produced embryos differ markedly from their in vivo counterparts, and these differences are associated with lower pregnancy outcomes and more health issues after birth. Nowadays, researchers consider the oviduct as the most suitable microenvironment for early embryonic development and a substantial effort is made to understand its dynamic, species-specific functions. In this review, we touch on the origin and molecular components of the oviductal fluid in mammals, where recent progress has been made thanks to the wider use of mass spectrometry techniques. Some of the factors and processes known to regulate oviductal secretions, including the embryo itself, as well as ovulation, insemination, endogenous and exogenous hormones, and metabolic and heat stress, are summarized. Special emphasis is laid on farm animals because, owing to the availability of sample material and the economic importance of fertility in livestock husbandry, a large part of the work on this topic has been carried out in domestic animals used for dairy and/or meat production.

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“…The Benjamini-Hochberg correction for multiple tests was further applied to adjust the derived p-values 18 . Only functional categories and pathways with p-values under a threshold of 0.05 were considered signi cant 19 .…”

Section: Data Processingmentioning

confidence: 99%

Comparative proteomic analysis of mild and severe endometritis determined by LC-MS/MS

Jiang

Cong

et al. 2022

Preprint

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Xinong Saanen goats are among the most common dairy goats in China and serve as major sources of milk in the Chinese dairy industry. Escherichia coli is a Gram-negative bacterium frequently implicated in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria and is involved in pathogenic processes that lead to post-partum metritis and endometritis in dairy goats. After binding to toll-like receptor 4, LPS induces reactions in the endometrium, leading to acute or chronic inflammation, which impairs reproductive function. The purpose of this study was to evaluate the effects of LPS on goat endometrial epithelial cells (gEECs) and the changes that occur in response to LPS. The proteomic map of gEECs exposed to different concentrations of LPS was studied using unlabeled liquid chromatography with tandem mass spectrometry. A total of 1178 proteins were identified in all samples, and 13 differentially expressed proteins were subjected to quantitative and gene ontology functional annotation analyses. In conclusion, this study shows that specific proteins expressed in the goat uterus are altered according to the concentration of LPS.

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Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Cited by 16 publications

References 48 publications

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Comparative proteomic analysis of mild and severe endometritis determined by LC-MS/MS

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