Proteome readjustments in the apoplastic space of Arabidopsis thaliana ggt1 mutant leaves exposed to UV-B radiation

Trentin, Anna Rita; Pivato, Micaela; Mehdi, Syed Muhammad Muntazir; Barnabas, Leonard; Giaretta, Sabrina; Fabrega-Prats, Marta; Prasad, Dinesh; Arrigoni, Giorgio; Masi, Antonio

doi:10.3389/fpls.2015.00128

Cited by 31 publications

(25 citation statements)

References 67 publications

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“…(A) Number of CWPs identified in experiment 1 (1DE) vs. number of CWPs identified in experiment 2 (shotgun). (B) Comparison of the number of CWPs identified in this study (destructive method) and in previous studies (non‐destructive method) . (C) Distribution (%) of CWPs identified in the overall rosette cell wall proteome (non‐destructive and destructive methods) in functional classes according to bioinformatic prediction of functional domains: PAC (proteins acting on carbohydrates), OR (oxido‐reductases), P (proteases), LM (proteins possibly related to lipid metabolism), ID (proteins with interactions domains with carbohydrates or proteins), S (proteins possibly involved in signaling), SP (structural proteins), M (miscellaneous proteins), UF (proteins of yet unknown function).…”

Section: Number Of Proteins Specific To the Enlarged Rosette Cell Walmentioning

confidence: 86%

An enlarged cell wall proteome of Arabidopsis thaliana rosettes

et al. 2016

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Plant cells are surrounded by cell walls playing many roles during development and in response to environmental constraints. Cell walls are mainly composed of polysaccharides (cellulose, hemicelluloses and pectins), but they also contain proteins which are critical players in cell wall remodeling processes. Today, the cell wall proteome of Arabidopsis thaliana, a major dicot model plant, comprises more than 700 proteins predicted to be secreted (cell wall proteins-CWPs) identified in different organs or in cell suspension cultures. However, the cell wall proteome of rosettes is poorly represented with only 148 CWPs identified after extraction by vacuum infiltration. This new study allows enlarging its coverage. A destructive method starting with the purification of cell walls has been performed and two experiments have been compared. They differ by the presence/absence of protein separation by a short 1D-electrophoresis run prior to tryptic digestion and different gradient programs for peptide separation before mass spectrometry analysis. Altogether, the rosette cell wall proteome has been significantly enlarged to 361 CWPs, among which 213 newly identified in rosettes and 57 newly described. The identified CWPs fall in four major functional classes: 26.1% proteins acting on polysaccharides, 11.1% oxido-reductases, 14.7% proteases and 11.7% proteins possibly related to lipid metabolism.

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Section: Number Of Proteins Specific To the Enlarged Rosette Cell Walmentioning

confidence: 86%

An enlarged cell wall proteome of Arabidopsis thaliana rosettes

et al. 2016

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“…The several PR-1 proteins, despite their unknown biological functions, are often used as markers of biotic stress [20,71], but in this review they were also found in abiotic stress and the non-challenged conditions [12,19,37,72,73]. All PR-1 proteins have similar structure and are classified in the same family on the basis of sequence homology although they can have different properties and differ substantially in biological activity [64].…”

Section: The Biological Processes Detected In the Ecsmentioning

confidence: 99%

“…In poplar and Arabidopsis this protein was found together with a cysteine-rich repeat secretory protein 38-like which seems to be structurally similar with a lipid transfer protein from tomato and can, thus, be classified as a PR-14 protein [21,73]. …”

Section: The Biological Processes Detected In the Ecsmentioning

confidence: 99%

“…The most abundant ones were the reticuline-like oxidases (berberine bridge enzyme-like and reticuline oxidase) and cinnamyl-alcohol dehydrogenase. Reticuline-like oxidases (FAD-family) are monolignol oxidoreductases that play a role in monolignol metabolism and lignin formation [73,107]. These enzymes may participate in the mobilization and oxidation of monolignols (aromatic allylic alcohols, such as coumaryl-, sinapyl-, and coniferyl alcohol), to the corresponding aldehydes.…”

Section: The Biological Processes Detected In the Ecsmentioning

confidence: 99%

“…They interact selectively and non-covalently with any carbohydrate, which includes monosaccharides, oligosaccharides, and polysaccharides, as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom (UniProtKB database). They have been detected in ECS of various plants and can be involved either on the cell wall reinforcement/organization or in cell wall biogenesis [19,20,73,101,114]. …”

Section: The Biological Processes Detected In the Ecsmentioning

confidence: 99%

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Protein Dynamics in the Plant Extracellular Space

et al. 2016

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The extracellular space (ECS or apoplast) is the plant cell compartment external to the plasma membrane, which includes the cell walls, the intercellular space and the apoplastic fluid (APF). The present review is focused on APF proteomics papers and intends to draw information on the metabolic processes occurring in the ECS under abiotic and biotic stresses, as well as under non-challenged conditions. The large majority of the proteins detected are involved in “cell wall organization and biogenesis”, “response to stimulus” and “protein metabolism”. It becomes apparent that some proteins are always detected, irrespective of the experimental conditions, although with different relative contribution. This fact suggests that non-challenged plants have intrinsic constitutive metabolic processes of stress/defense in the ECS. In addition to the multiple functions ascribed to the ECS proteins, should be considered the interactions established between themselves and with the plasma membrane and its components. These interactions are crucial in connecting exterior and interior of the cell, and even simple protein actions in the ECS can have profound effects on plant performance. The proteins of the ECS are permanently contributing to the high dynamic nature of this plant compartment, which seems fundamental to plant development and adaptation to the environmental conditions.

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