2015
DOI: 10.2116/analsci.31.935
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Proteomic Analysis after Sequential Extraction of Matrix Proteins in Urinary Stones Composed of Calcium Oxalate Monohydrate and Calcium Oxalate Dihydrate

Abstract: In this study, we performed proteomic analysis following sequential protein extraction on calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) urinary stones to determine the specific matrix proteins according to the crystal components of the stones. After X-ray and IR analysis of 13 urinary stones, matrix proteins were sequentially extracted with KCl, formic acid, guanidine-HCl, and EDTA, before SDS-electrophoresis followed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/M… Show more

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Cited by 12 publications
(15 citation statements)
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“…The characterization of proteins in urinary stones has been limited owing to the poor solubility of the protein-crystal complex. However, proteomic analyses of both urinary proteins and human stone matrices have been published 87,[94][95][96][97][98] . The protein composition of stones and the stone matrix seems to be quite variable, even among similar stone types, suggesting patient specificity in stone composition 87 .…”
Section: Impuritiesmentioning
confidence: 99%
See 1 more Smart Citation
“…The characterization of proteins in urinary stones has been limited owing to the poor solubility of the protein-crystal complex. However, proteomic analyses of both urinary proteins and human stone matrices have been published 87,[94][95][96][97][98] . The protein composition of stones and the stone matrix seems to be quite variable, even among similar stone types, suggesting patient specificity in stone composition 87 .…”
Section: Impuritiesmentioning
confidence: 99%
“…A range of stone matrix proteins have been identified to date, including those associated with immune responses, inflammation, tissue injury, and tissue repair. The most common and abundant proteins detected in the matrix of all studied stone types (calcium oxalate, calcium phosphate, uric acid, and struvite stones) include serum albumin, apolipoproteins, calgranulins (also known as S100 proteins), and haemoglobin subunits 87,[93][94][95][96][97][98] . Proteins involved in inflammatory and fibrotic processes -which are usually associated with crystal deposition in the kidneys -are prominent in the stone matrix of all stone types studied 87,[93][94][95][96][97][98] .…”
Section: Impuritiesmentioning
confidence: 99%
“…Most of these dominant matrix proteins have been reported by others in earlier stone matrix proteomic studies [4,[19][20][21][22][23][24][25]. Albumin (ALBU) and Tamm-Horsfall protein (uromodulin, UROM), the most highly abundant urine proteins (20% and 13%, respectively) [15], were both found in all stone matrix samples, but at lower relative abundance (6% and 3%, respectively).…”
Section: Resultsmentioning
confidence: 57%
“…The crystal phase and organic substances contained in the kidney stones are identified using Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, energy dispersive X-ray spectrometry (SEM-EDX), mass spectroscopy, etc. [6, 7]. However, identification using powdering samples had limitations; the spatial information of stones, in other words, the historical data of the stones, was lost.…”
Section: Introductionmentioning
confidence: 99%