Proteomic analysis of Bacillus cereus growing in liquid soil organic matter

Luo, Yun; Vilain, Sébastien; Voigt, Birgit; Albrecht, Dirk; Hecker, Michael; Brözel, Volker S.

doi:10.1111/j.1574-6968.2007.00692.x

Cited by 21 publications

(10 citation statements)

References 43 publications

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“…Based on morphological examination, physiological tests, and molecular techniques, applied isolated strains were identified as B. cereus and E. acetylicum. B. cereus is usually known as a soil bacteria [7], capable of growing on shell waste [1]. Exiguobacterium bacteria are also found in diverse environments [18].…”

Section: Discussionmentioning

confidence: 99%

Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum

Sorokulova

Krumnow

Globa

et al. 2009

J Ind Microbiol Biotechnol

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Two bacterial cultures were isolated and tested for degradation of shrimp shell waste. According to morphological examination, physiological tests, and applied molecular techniques, isolates were identified as Bacillus cereus and Exiguobacterium acetylicum. Both strains were cultivated separately in flasks with 100 mL of shrimp shell waste broth (3% of washed, dried and ground shrimp shell waste in tap water, pH 7.0) at 37 degrees C. At determined periods of time, deproteinization and demineralization of residuals were measured. Fermentation of 3% shell waste with B. cereus indicated 97.1% deproteinization and 95% demineralization. For E. acetylicum, the level of deproteinization and demineralization was 92.8 and 92%, respectively. Protein content was reduced from 18.7 to 5.3% with B. cereus and to 7.3% with E. acetylicum. No additional supplements were used during the fermentation of shell waste. B. cereus strain showed higher efficacy in decomposition of shell waste and was used for large-scale fermentation in 12 L of 10% shrimp shell waste broth. Incubation of bacteria with shell waste during 14 days at 37 degrees C resulted in 78.6% deproteinization and 73% demineralization. High activity of isolated cultures in decomposition of shrimp shell waste suggests broad potential for application of these bacteria in environmentally friendly approaches to chitin extraction from chitin-rich wastes.

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Section: Discussionmentioning

confidence: 99%

Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum

Sorokulova

Krumnow

Globa

et al. 2009

J Ind Microbiol Biotechnol

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“…S3b). The genus Bacillus is a bacterial r-strategist (26), which has a higher maximum growth rate (13), and is able to rapidly adapt to changes in environmental conditions (19). The marked increase observed in the relative contribution of the genus Bacillus in the present study suggests that this genus is of considerable ecological importance to the sediments.…”

Section: Discussionmentioning

confidence: 99%

Linking Temporal Changes in Bacterial Community Structures with the Detection and Phylogenetic Analysis of Neutral Metalloprotease Genes in the Sediments of a Hypereutrophic Lake

et al. 2014

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We investigated spatial and temporal variations in bacterial community structures as well as the presence of three functional proteolytic enzyme genes in the sediments of a hypereutrophic freshwater lake in order to acquire an insight into dynamic links between bacterial community structures and proteolytic functions. Bacterial communities determined from 16S rRNA gene clone libraries markedly changed bimonthly, rather than vertically in the sediment cores. The phylum Firmicutes dominated in the 4–6 cm deep sediment layer sample after August in 2007, and this correlated with increases in interstitial ammonium concentrations (p < 0.01). The Firmicutes clones were mostly composed of the genus Bacillus. npr genes encoding neutral metalloprotease, an extracellular protease gene, were detected after the phylum Firmicutes became dominant. The deduced Npr protein sequences from the retrieved npr genes also showed that most of the Npr sequences used in this study were closely related to those of the genus Bacillus, with similarities ranging from 61% to 100%. Synchronous temporal occurrences of the 16S rRNA gene and Npr sequences, both from the genus Bacillus, were positively associated with increases in interstitial ammonium concentrations, which may imply that proteolysis by Npr from the genus Bacillus may contribute to the marked increases observed in ammonium concentrations in the sediments. Our results suggest that sedimentary bacteria may play an important role in the biogeochemical nitrogen cycle of freshwater lakes.

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“…Analysis by NMR of glomalin-related proteins extracted from soil also indicated the presence of humic substances (Schindler et al 2007). The interference of organic impurities on the characterization of proteins of S. maltophila inoculated into soils was reported by Taylor and Williams (2009), whereas no interferences in the characterization of the proteome of Bacillus cereus grown on soil organic matter extracts were reported by Luo et al (2007). Overall, the 2-DE proteome analyses demonstrate that proteome characterization of soil microorganisms can be strongly influenced by the reactivity of the soil inorganic and organic phases.…”

Section: Ms Analysismentioning

confidence: 78%

Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34

et al. 2011

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Cupriavidus metallidurans CH34 is a completely sequenced soil-borne beta-proteobacterium with known genome and proteome. Comparative 2-D electrophoresis and protein mass spectrometry were used to compare the proteome of C. metallidurans CH34 from liquid culture and after incubation for 1, 3, and 12 days in microcosms containing quartz sand, kaolinite, montmorillonite, or an artificial soil. Results showed that proteome from liquid culture was similar to CH34 proteins extracted from sand and kaolinite, whereas the proteins extracted from artificial soil differed significantly and no proteins were detected from C. metallidurans CH34 incubated in the montmorillonite microcosms. Protein recovery decreased on prolonging incubation time in all microcosms. Mass spectrometry identification showed that the trend of lower recovery upon incubation time was independent on the putative function of protein. These results suggest that the soil solid phase influences the protein recovery and soil proteomic analysis and that distinction between protein recovery and protein expression in soil will be a challenging for soil proteomic researchers. © 2011 Springer-Verlag

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Proteomic analysis of Bacillus cereus growing in liquid soil organic matter

Cited by 21 publications

References 43 publications

Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum

Efficient decomposition of shrimp shell waste using Bacillus cereus and Exiguobacterium acetylicum

Linking Temporal Changes in Bacterial Community Structures with the Detection and Phylogenetic Analysis of Neutral Metalloprotease Genes in the Sediments of a Hypereutrophic Lake

Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34

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