Proteomic analysis of CD4+ T-lymphocytes in patients with asthma between typical therapy (controlled) and no typical therapy (uncontrolled) level

Abstract

<jats:p> T-lymphocyte (T-LC)-derived cytokines have been implicated in asthmatic pathogenesis. Proteomic technology is now widely accepted as a complementary technology to genetic profiling. We investigated the changes of proteins in T-LC of asthmatic patients from the no typical therapy (uncontrolled) to typical therapy (controlled) level by using standard proteome technology. Methods: The proteins of CD4+ T-LC were isolated from the whole blood of six asthmatic patients from uncontrolled to controlled levels over 3 months. Two-dimensional polyacrylamide gel electrophoresis was performed and coomassie blue stained protein spots were comparatively analyzed by using an image analyzer. Some differentially expressed spots were identified by liquid chromatography/mass spectrometry and database search. Our results showed that 13 proteins showed different expression. Six protein spots in the CD4+ T-LC of the uncontrolled asthmatic patients were increased and 7 spots were decreased compared to those of the controlled subjects. In conclusion, the proteomic examination of the CD4+ T-LC revealed some differentially expressed proteins in the uncontrolled and controlled asthmatic patients. The possibility of using the differentially expressed proteins as important biomarkers and therapeutic targets warrants further study. </jats:p>

Respiratory Proteomics: From Descriptive Studies to Personalized Medicine

“…Among 14 differentially expressed proteins they identified S100A9 (calgranulin B) as a specific marker for neutrophilic uncontrolled asthma . CD4+ T lymphocytes derived from uncontrolled asthma patients have also been investigated and found to produce 13 differentially expressed proteins including vimentin, which was described in O’Neil et al’s report . While inhaled medications keep asthma symptoms under control in most patients, a minority of those with severe asthma do not improve following medication.…”

Biomarker discovery in asthma and COPD by proteomic approaches

“…Gel-based methods represent the most widely used proteomic approach and are based on mono-or bidimensional gel electrophoresis (1DG or 2DG) combined with the isolation and identification of the protein bands/spots of interest by means of MS. In particular, as shown in Tables 1 and 2, asthma and COPD proteomic investigations are mainly supported by gel-based approaches, among which a high percentage is represented by 2DG and its variant, 2D-DIGE [61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76].…”

Proteomic profiling of lymphocytes in autoimmunity, inflammation and cancer

“…Figure 2 demonstrates some of the identified proteins and their role in asthma. To investigate the changes of proteins in T lymphocytes of asthmatic patients after no typical therapy (uncontrolled) to typical therapy (controlled) over 3 months, 7 proteins increased and 6 decreased in CD4 + T lymphocytes from uncontrolled asthmatic patients, including HSP-70, HSP-90, fibrinogen β chain, tropomyosin 3, ATP-dependent DNA helicase II, β-actin, vimentin, Rho GDP dissociation inhibitor beta, enolase 1, calreticulin precursor, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA), or peroxiredoxin 2, as compared to those from controls [50]. Two studies showed that HSP-70 increased and vimentin decreased in asthmatics and uncontrolled asthmatics.…”