Proteomic analysis of developing rye grain with contrasting resistance to preharvest sprouting

Abstract

Significant differences in the two-dimensional electrophoresis patterns of proteins from developing rye grain were found to be associated with resistance and susceptibility to preharvest sprouting (PHS). Mass spectrometry of individual spots showing different abundance in PHS-resistant and PHS-susceptible lines identified proteins involved in: reaction to biotic and abiotic stresses, including oxidative stress, energy metabolism and regulation of gene expression. Highly differentiated abundance of proteins found in developing grain suggest that the diversification of processes leading to developing PHS resistance or PHS susceptibility starts from an early stage of grain development. A part of the identified proteins in rye grain were also reported to be associated with PHS in wheat and rice, which suggests that some mechanisms affecting precocious germination might be common for different cereal species.Electronic supplementary materialThe online version of this article (doi:10.1007/s13353-012-0127-8) contains supplementary material, which is available to authorized users.

QTL mapping for benzoxazinoid content, preharvest sprouting, α-amylase activity, and leaf rust resistance in rye (Secale cereale L.)

“…The present experiment however did not identify the QTL for PHS on chromosomes 3RS, 6RL, and 7RS detected earlier by using both QTL mapping and BSG analysis. A proteomic analysis of lines representing the resistant and susceptible lines of the 541 × Ot1-3 cross showed that many identified proteins represented defensive functions against microbial and insect pests [ 28 , 29 ]. This should not be surprising as only the intact grain surface without any destructive effects of pets’ activities can resist water infiltration, which directly induces sprouting and the α-amylase production.…”

Identification of Kernel Proteins Associated with the Resistance to Fusarium Head Blight in Winter Wheat (Triticum aestivum L.)

“…The proteomic protocol used, including 2-DE and MS to identify differentially accumulated kernel proteins between the RL and SL of wheat, was the same as that described in detail by Masojć and Kosmala [31] and Masojć et al [32] . Protein extraction was performed according to the method of Hurkman and Tanaka [33] using 0.7 M sucrose, 0.5 M TRIS, 30 mM HCl, 50 mM EDTA, 2% DTT, and 0.1 M KCl as components of the extraction buffer.…”

Two Festuca Species—F. arundinacea and F. glaucescens—Differ in the Molecular Response to Drought, While Their Physiological Response Is Similar

“…Protein accumulation profiles of three enzymes of the Calvin cycle (pFBA, pPGK, pGAPDH) and seven antioxidant enzymes including glutathione reductase (GR, AS06 181), chloroplastic glutathione peroxidase (GPX, AS04 055), chloroplastic Fe-dependent superoxide dismutase (Fe-SOD, AS06 125), chloroplastic Cu/Zn superoxide dismutase (Cu/Zn-SOD, AS06 170), manganese superoxide dismutase (Mn-SOD, AS09 524), L-ascorbate peroxidase (APX, AS08 368) and catalase (CAT, AS09 501), were analyzed. Total proteins were extracted from pooled leaves using Hurkman and Tanaka protocol with slight modifications [4,62,63]. Briefly, 200 mg of powdered tissue was homogenized with 500 µL of extraction buffer (0.7 M sucrose, 0.5 M TRIS, 30 mM HCl, 50 mM EDTA, 2% DTT, and 0.1 M KCl).…”