Proteomic analysis of egg white proteins during the early phase of embryonic development

Qiu, Ning; Ma, Meihu; Cai, Zhaoxia; Jin, Yongguo; Huang, Xi; Huang, Qun; Sun, Shuguo

doi:10.1016/j.jprot.2011.12.037

Cited by 63 publications

(91 citation statements)

References 43 publications

(67 reference statements)

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“…We studied TCam-2 seminoma cells considering that this particular cancer develops from Carcinoma in situ, a pre-invasive lesion with origin during the embryonic development because of the delayed or blocked differentiation of PGCs or gonocytes, due to an alteration of the testicular embryonic niche [6,7]. To provide embryonic cues we used a medium added with egg albumen (EW) whose microenvironment is able to regulate chicken embryo protein biosyntesis [30]. Firstly we found that EW modifies cell spreading rate and cell-substrate adhesion without affecting proliferation and survival indexes of TCam-2 cells in 40-50 minutes cultures.…”

Section: Discussionmentioning

confidence: 99%

TCam-2 Seminoma Cells Exposed to Egg-Derived Microenvironment Modify Their Shape, Adhesive Pattern and Migratory Behaviour: A Molecular and Morphometric Analysis

et al. 2013

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Seminoma is one of the most common Testicular Germ Cell Tumours that originates during embryonic development due to an alteration of the local niche that in turn results in a delayed or blocked differentiation of Primordial Germ Cells. The block of differentiation is actually a common way to develop cancer disease as postulated by the "embryonic rest theory of cancer". In agreement with this theory different studies have demonstrated that embryonic cues display the capacity of reprogramming aggressive cancer cells towards a less aggressive phenotype. Herein we investigate the ability of a culture medium added with 10% egg albumen (EW, Egg White) to modulate seminoma cell phenotype and behaviour, by ensuring a proper set of morphogenetic signals. We chose to use the TCam-2 seminoma cell line that has been established as the only available cell line, obtained from a primary testicular seminoma. EW is able to: 1) modify TCam-2 cell spreading rate and cell-substrate adhesion without affecting proliferation and survival indexes; 2) modulate TCam-2 actin distribution pattern increasing cortical localization of actin filaments; 3) increase TCam-2 cell-cell junction capability; 4) decrease both chemo-sensitive and collective TCam-2 migratory behaviour. According to these observations morphometric fractal analysis revealed the ability of EW to increase Circularity and Solidity parameters and, consequently, to decrease Fractal dimension. Prompted by these observations we hypothesize that EW treatment could rescue, at least in part, the neoplastic-metastatic behaviour of seminoma cells.

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Section: Discussionmentioning

confidence: 99%

TCam-2 Seminoma Cells Exposed to Egg-Derived Microenvironment Modify Their Shape, Adhesive Pattern and Migratory Behaviour: A Molecular and Morphometric Analysis

et al. 2013

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“…Avian egg white is comprised of around 88.5% water and 11.5% solids, of which proteins constitute 10.5% of total content (Burley and Vadehra, ). These proteins show not only great functional and structural heterogeneity (Stevens,; Qiu et al, ) but there is also evidence that most of them vary significantly within as well as between species (Feeney et al, ; Shawkey et al, ; D'Alba et al, ; Giraudeau et al, ). Although, mostly antibacterial function of egg white proteins has been studied (e.g.…”

mentioning

confidence: 99%

Effects of experimentally increased in ovo lysozyme on egg hatchability, chicks complement activity, and phenotype in a precocial bird

et al. 2015

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In birds, spectrum of egg white proteins deposited into the egg during its formation are thought to be essential maternal effects. Particularly, egg white lysozyme (LSM), exhibiting great between and within species variability, is considered to be essential for developing avian embryos due to its physiological, antimicrobial, and innate immune defense functions. However, there have been few studies investigating effects of LSM on early post-hatching phenotype, despite its broad physiological and protective role during embryogenesis. Here, we test how experimentally increased concentrations of egg white LSM affect hatchability in Japanese quail (Coturnix japonica) and chick phenotype immediately after hatching (particularly body weight, tarsus length, plasma LSM concentration, and plasma complement activity). Chicks from eggs with increased LSM concentration displayed reduced tarsus length compared to chicks from control eggs while hatchability, body weight and plasma LSM concentration were unaffected. It is worth noting that no effect of increased in ovo lysozyme on eggs hatchability could be related to pathogen-free environment during artificial incubation of experimental eggs causing minimal pressure on embryo viability. While tangible in vivo mechanisms during avian embryogenesis remain to be tested, our study is the first to document experimentally that egg white LSM appears to have growth-regulation role during embryo development, with possible underlying phenotypic consequences in the early post-hatching period in precocial birds.

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“…Proteomic studies performed on the egg white at different stages of the embryonic growth did not reveal any significant alteration of ovalbumin during incubation although some degradation products as well as protein-protein complexes have been identified [11]. The current hypothesis is that ovalbumin would serve as a source of amino-acids for the developing embryo.…”

Section: Functional Characterizationmentioning

confidence: 75%

“…The role of OVAY has not been explored yet but some have shown that its abundance was affected during incubation [9,11] and that it was associated with fertilized eggs. Indeed, various protein spots corresponding to partly proteolyzed (or possibly activated) OVAY showed more than a 10-fold increase (P < 0.01) in abundance in freshly laid fertilized chicken egg whites compared with unfertilized egg whites [10].…”

Section: Functional Characterizationmentioning

confidence: 99%

“…The two related proteins OVAY and OVAX have been identified in egg white in 2006 [8] and although their physicochemical properties have been characterized recently, their physiological functions remains undefined. There is however, increasing data in literature regarding their potential involvement in some aspects of fertilization or embryonic development [9][10][11] or egg defense [12].…”

Section: Introductionmentioning

confidence: 99%

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The Family Secrets of Avian Egg-Specific Ovalbumin and Its Related Proteins Y and X

Silva¹,

Beauclercq²,

Harichaux³

et al. 2015

Biology of Reproduction

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The ovalbumin gene family in Gallus gallus is composed of three homologous genes located within a 46 kb locus on chromosome 2: ovalbumin, ovalbumin-related protein Y (OVAY), and ovalbumin-related protein X (OVAX) genes. The expression of these genes in hen oviduct is under estrogen control, but their relative hormonal responsiveness and subsequent protein concentration in egg, is distinctive. Interestingly, all three proteins lack the classical signal peptide for secretion. Ovalbumin, OVAX, and OVAY belong to the serine protease inhibitor (serpin) family whose members share a common tertiary structure. Ovalbumin and OVAX are one of the few members of this family that do not express any protease inhibition activity whereas OVAY has been predicted to be inhibitory, by comparison with the consensus sequence for inhibitory serpins. In contrast to ovalbumin and OVAY, OVAX interacts with heparin, a negatively charged glycosaminoglycan, via a positively charged domain exposed at the surface of the molecule. Ovalbumin is the major egg white protein and might be a source of amino acids for the developing embryo. The physiological function of OVAY is not known, but recent data have revealed a possible role of this protein in early embryonic development. Considering the antibacterial activities of OVAX, this protein might play a role in egg defense. This review sheds light on the expression, biochemistry, and structural specificities of these three highly similar paralogs. It gives new clues in favor of diverging functions, which are likely to have arisen by duplication events from a common ancestral gene.

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Proteomic analysis of egg white proteins during the early phase of embryonic development

Cited by 63 publications

References 43 publications

TCam-2 Seminoma Cells Exposed to Egg-Derived Microenvironment Modify Their Shape, Adhesive Pattern and Migratory Behaviour: A Molecular and Morphometric Analysis

TCam-2 Seminoma Cells Exposed to Egg-Derived Microenvironment Modify Their Shape, Adhesive Pattern and Migratory Behaviour: A Molecular and Morphometric Analysis

Effects of experimentally increased in ovo lysozyme on egg hatchability, chicks complement activity, and phenotype in a precocial bird

The Family Secrets of Avian Egg-Specific Ovalbumin and Its Related Proteins Y and X

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