Proteomic analysis of ethanol-induced embryotoxicity in cultured post-implantation rat embryos

Usami, Makoto; Mitsunaga, Katsuyoshi; Irie, Tomohiko; Miyajima, Atsuko; Doi, Osamu

doi:10.2131/jts.39.285

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…It was noted, however, that the identified proteins included alpha-fetoprotein [standard spot numbers (SSPs 4818, 4831)], phosphorylated cofilin-1 (SSP 6001), and serum albumin (SSPs 4832, 5805). These three proteins have been considered candidate protein biomarkers that may be involved in embryotoxic mechanisms, as reported in our previous studies (Usami et al, 2014;Usami et al, 2009;Usami et al, 2008).…”

Section: Effects Of Vpa On Embryonic Protein Expressionmentioning

confidence: 80%

“…To date, we have examined protein expression changes in cultured embryos exposed to certain developmental toxicants, i.e., selenium, indium, and ethanol, and found candidate protein biomarkers that may be involved in embryotoxic mechanisms (Usami et al, 2008;Usami et al, 2009;Usami et al, 2014). It is, however, still important to accumulate proteomic analysis data of developmental toxicants.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos

Usami

Takamatsu

Kazama

et al. 2017

Fundam. Toxicol. Sci.

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-Protein expression changes were examined in day 10.5 rat embryos cultured for 24 hr in the presence of valproic acid (VPA), using two-dimensional electrophoresis and mass spectrometry. Exposure to VPA at an embryotoxic concentration of 1.2 mM resulted in quantitative changes in many embryonic protein spots (22 decreased and 29 increased). For the increased protein spots, 10 proteins were identified, including alpha-fetoprotein, phosphorylated cofilin-1, and serum albumin. These proteins are candidate protein biomarkers that may be involved in embryotoxic mechanisms.

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Section: Effects Of Vpa On Embryonic Protein Expressionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos

Usami

Takamatsu

Kazama

et al. 2017

Fundam. Toxicol. Sci.

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“…In this context, it is intriguing that ethanol and indium also increased phosphorylated cofiline 1 in cultured rat embryos (Usami et al. , ). However, indium did not have inhibitory effects on the migration of cNCCs or tNCCs in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…We selected developmentally toxic chemicals on the basis of our interest in our related study such as proteomics of embryos (Usami et al. , , ) and metabolomics of hepatocytes (Kim et al. ) since there was little information about the effects of chemicals on the migration of cNCCs.…”

Section: Introductionmentioning

confidence: 99%

“…We selected developmentally toxic chemicals on the basis of our interest in our related study such as proteomics of embryos (Usami et al , 2009(Usami et al , 2014a and metabolomics of hepatocytes (Kim et al 2014) since there was little information about the effects of chemicals on the migration of cNCCs. However, we considered that the chemicals include ones that might affect cNCC migration, e.g., ethanol, and selenate, and ones that might not, e.g., indium, and tributyltin, which was speculated from their potential to cause craniofacial abnormality.…”

Section: Introductionmentioning

confidence: 99%

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Effects of 13 developmentally toxic chemicals on the migration of rat cephalic neural crest cells in vitro

Usami

Mitsunaga

Miyajima

et al. 2016

Congenital Anomalies

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The inhibition of neural crest cell (NCC) migration has been considered as a possible pathogenic mechanism underlying chemical developmental toxicity. In this study, we examined the effects of 13 developmentally toxic chemicals on the migration of rat cephalic NCCs (cNCCs) by using a simple in vitro assay. cNCCs were cultured for 48 h as emigrants from rhombencephalic neural tubes explanted from rat embryos at day 10.5 of gestation. The chemicals were added to the culture medium at 24 h of culture. Migration of cNCCs was measured as the change in the radius (radius ratio) calculated from the circular spread of cNCCs between 24 and 48 h of culture. Of the chemicals examined, 13-cis-retinoic acid, ethanol, ibuprofen, lead acetate, salicylic acid, and selenate inhibited the migration of cNCCs at their embryotoxic concentrations; no effects were observed for acetaminophen, caffeine, indium, phenytoin, selenite, tributyltin, and valproic acid. In a cNCC proliferation assay, ethanol, ibuprofen, salicylic acid, selenate, and tributyltin inhibited cell proliferation, suggesting the contribution of the reduced cell number to the inhibited migration of cNCCs. It was determined that several developmentally toxic chemicals inhibited the migration of cNCCs, the effects of which were manifested as various craniofacial abnormalities.

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Effects of Alcohol on Embryo/Fetal Development

Guerri¹,

Pascual²

2017

Reproductive and Developmental Toxicology

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Proteomic analysis of ethanol-induced embryotoxicity in cultured post-implantation rat embryos

Cited by 6 publications

References 20 publications

Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos

Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos

Effects of 13 developmentally toxic chemicals on the migration of rat cephalic neural crest cells in vitro

Effects of Alcohol on Embryo/Fetal Development

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