Proteomic analysis of human follicular fluid: A new perspective towards understanding folliculogenesis

Oocyte environment: follicular fluid and cumulus cells are critical for oocyte health

“…Many of these proteins involve metabolism, extracellular matrix (ECM) remodeling, and immune responses (145) and also have been identified in other studies (146,147), along with additional biomarkers of oocyte competence (i.e., superoxide dismutase activity 150)). Additional examination of the human follicular fluid proteome in reproductive disorders like endometriosis (151) and ovarian hyperstimulation syndrome (152) could reveal new information about disease pathology and additional biomarkers of oocyte quality.…”

Ovarian response is affected by a specific histidine-rich glycoprotein polymorphism: a preliminary study

“…(2012) studied the contents of these microvesicles in mares and found th a t they contained an HRG-like protein (da Silveira e t al., 2012). H istidine-rich glycoprotein has previously been iden tifie d in human follicu la r fluid (Ambekar et al, 2013; Nordqvist e t a l., 2010). A dditionally, high levels o f VEGF in fo llic u la r flu id have been associated w ith lo w fe r tiliz a tio n rates (M alamitsi-Puchner e t a l., 2001) and poor q u a lity embryos (Barroso e t a l., 1999).…”

The Use of Proteomics in Assisted Reproduction

“…In addition, nine out of these 320 proteins (tumor necrosis factor, alpha-induced protein 6, angiogenin, ribonuclease, RNase A family 5, fibronectin 1, serpin peptidase inhibitor clade E, pentraxin 3, glutathione peroxidase 3, cathepsin B, superoxide dismutase and multimerin 2) were previously reported markers of oocyte quality and IVF outcome. The majority of the identified proteins were functionally classified in extracellular matrix proteins, components of inflammation and coagulation, proteins involved in angiogenesis and, mostly, enzymes (93).…”