Proteomic analysis of protein expression in Lactobacillus plantarum in response to alkaline stress

Lee, Ki-Beom; Rho, Beom-Seop; Pi, KyungBae; Kim, Ho-Jin; Choi, Yun‐Jaie

doi:10.1016/j.jbiotec.2011.02.008

Cited by 24 publications

(17 citation statements)

References 33 publications

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“…Our previous work showed that in L. crispatus ST1 cells, the pH-induced release of enolase and GAPDH is not associated with de novo protein synthesis (4). Proteomic studies have shown that growth under alkaline stress conditions increases the cellular content of enolase but reduces the amount of GS of L. plantarum (37), whereas here, the two proteins were released from L. crispatus ST1 cells by LL-37 treatment and in the pH 8 buffer in the same apparent ratio as they occurred in lysed cell samples.…”

Section: Discussionmentioning

confidence: 99%

Glutamine Synthetase and Glucose-6-Phosphate Isomerase Are Adhesive Moonlighting Proteins of Lactobacillus crispatus Released by Epithelial Cathelicidin LL-37

Kainulainen

Loimaranta

Pekkala

et al. 2012

Journal of Bacteriology

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c Glutamine synthetase (GS) and glucose-6-phosphate isomerase (GPI) were identified as novel adhesive moonlighting proteins of Lactobacillus crispatus ST1. Both proteins were bound onto the bacterial surface at acidic pHs, whereas a suspension of the cells to pH 8 caused their release into the buffer, a pattern previously observed with surface-bound enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of L. crispatus. The pH shift was associated with a rapid and transient increase in cell wall permeability, as measured by cell staining with propidium iodide. A gradual increase in the release of the four moonlighting proteins was also observed after the treatment of L. crispatus ST1 cells with increasing concentrations of the antimicrobial cationic peptide LL-37, which kills bacteria by disturbing membrane integrity and was here observed to increase the cell wall permeability of L. crispatus ST1. At pH 4, the fusion proteins His 6 -GS, His 6 -GPI, His 6 -enolase, and His 6 -GAPDH showed localized binding to cell division septa and poles of L. crispatus ST1 cells, whereas no binding to Lactobacillus rhamnosus GG was detected. Strain ST1 showed a pH-dependent adherence to the basement membrane preparation Matrigel. Purified His 6 -GS and His 6 -GPI proteins bound to type I collagen, and His 6 -GS also bound to laminin, and their level of binding was higher at pH 5.5 than at pH 6.5. His 6 -GS also expressed a plasminogen receptor function. The results show the strain-dependent surface association of moonlighting proteins in lactobacilli and that these proteins are released from the L. crispatus surface after cell trauma, under conditions of alkaline stress, or in the presence of the antimicrobial peptide LL-37 produced by human cells.

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Section: Discussionmentioning

confidence: 99%

Glutamine Synthetase and Glucose-6-Phosphate Isomerase Are Adhesive Moonlighting Proteins of Lactobacillus crispatus Released by Epithelial Cathelicidin LL-37

Kainulainen

Loimaranta

Pekkala

et al. 2012

Journal of Bacteriology

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“…As the genomes of three strains of L. plantarum have been completely sequenced and published [17][18][19], proteomics has been widely applied to gain insight into the mechanisms used by L. plantarum to respond to heat [20], alkaline [21] and bile exposure [22], and into the adhesion process underlying probiotic properties [23].…”

Section: Introductionmentioning

confidence: 99%

Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective

Mazzeo¹,

Cacace²,

Peluso³

et al. 2012

Journal of Proteomics

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“…Cell surface-associated proteins were separated and identified by another group [8]. Key proteins in the adhesion of L. plantarum , and in the response to tannic acid, bile, and alkaline stress were also analyzed by proteomic methods [9], [10], [11], [12], [13]. These proteomic studies provide valuable data and pave the way for a more comprehensive insight into the molecular basis of L. plantarum .…”

Section: Introductionmentioning

confidence: 99%

A Reference Proteomic Database of Lactobacillus plantarum CMCC-P0002

Zhu¹,

Wei

Liu

et al. 2011

PLoS ONE

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Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum.

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Proteomic analysis of protein expression in Lactobacillus plantarum in response to alkaline stress

Cited by 24 publications

References 33 publications

Glutamine Synthetase and Glucose-6-Phosphate Isomerase Are Adhesive Moonlighting Proteins of Lactobacillus crispatus Released by Epithelial Cathelicidin LL-37

Glutamine Synthetase and Glucose-6-Phosphate Isomerase Are Adhesive Moonlighting Proteins of Lactobacillus crispatus Released by Epithelial Cathelicidin LL-37

Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective

A Reference Proteomic Database of Lactobacillus plantarum CMCC-P0002

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