Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae

Fang, Xianping; Chen, Wenyue; Xin, Ya; Zhang, Heng-Mu; Yan, Chengqi; Yu, Hong; Liu, Hui; Xiao, Wenfei; Wang, Shuzhen; Zheng, Guizhen; Liu, Hongbo; Jin, Liang; Ma, Huiqin; Ruan, Songlin

doi:10.1016/j.jprot.2012.05.022

Cited by 54 publications

(53 citation statements)

References 73 publications

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“…This down-regulation of photosynthesis during pathogen infection may be caused by the large accumulation of hexose sugars, which leads to feedback inhibition of the expression of some photosynthetic genes [24,25]. It is conform to the report by Xianping Fang et al [16]. …”

Section: Discussionsupporting

confidence: 77%

“…Two-dimensional (2-D) gel electrophoresis has emerged as a powerful tool for the study of plant–stress responses to plant–herbivore and plant–pathogen interactions [11-13]. It has been successfully applied to analysis of proteomics in different plant [14-16]. Despite the promising results of these and other studies, no investigation has been made into the identification of potential disease resistance-related proteins in the Marssonina apple blotch.…”

Section: Introductionmentioning

confidence: 99%

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Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)

Qiu

et al. 2014

Proteome Sci

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BackgroundApple, an invaluable fruit crop worldwide, is often prone to infection by pathogenic fungi. Identification of potentially resistance-conferring apple proteins is one of the most important aims for studying apple resistance mechanisms and promoting the development of disease-resistant apple strains. In order to find proteins which promote resistance to Marssonina coronaria, a deadly pathogen which has been related to premature apple maturation, proteomes from apple leaves inoculated with M. coronaria were characterized at 3 and 6 days post-inoculation by two dimensional electrophoresis (2-DE).ResultsOverall, 59 differentially accumulated protein spots between inoculation and non-inoculation were successfully identified and aligned as 35 different proteins or protein families which involved in photosynthesis, amino acid metabolism, transport, energy metabolism, carbohydrate metabolism, binding, antioxidant, defense and stress. Quantitative real-time PCR (qRT-PCR) was also used to examine the change of some defense and stress related genes abundance under inoculated conditions.ConclusionsIn a conclusion, different proteins in response to Marssonina coronaria were identified by proteomic analysis. Among of these proteins, there are some PR proteins, for example class III endo-chitinase, beta-1,3-glucanase and thaumatine-like protein, and some antioxidant related proteins including aldo/keto reductase AKR, ascorbate peroxidase and phi class glutathione S-transferase protein that were associated with disease resistance. The transcription levels of class III endo-chitinase (L13) and beta-1, 3-glucanase (L17) have a good relation with the abundance of the encoded protein’s accumulation, however, the mRNA abundance of thaumatine-like protein (L22) and ascorbate peroxidase (L28) are not correlated with their protein abundance of encoded protein. To elucidate the resistant mechanism, the data in the present study will promote us to investigate further the expression regulation of these target proteins.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)

Qiu

et al. 2014

Proteome Sci

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“…alternata pathogen. The expression pattern of beta-1,3-glucanase in apple leaves was similar to that in grapes infected with powdery mildew [37], although this phenomenon has not been observed in other host plants [33,38]. …”

Section: Discussionmentioning

confidence: 68%

“…The up- and down-regulation of RuBisCO (Spot 33) in susceptible and resistant leaves may be related to the complicated defence response to the pathogen. The down-regulation of other photosynthesis proteins (Spot 18) during pathogen infection may indicate some feedback inhibition of photosynthetic genes [33]. …”

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Pathogen-Responsive Proteins from Apple Leaves Induced by the Alternaria Blotch Alternaria alternata

2015

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Understanding the defence mechanisms used by apple leaves against Alternaria alternate pathogen infection is important for breeding purposes. To investigate the ultrastructural differences between leaf tissues of susceptible and resistant seedlings, in vitro inoculation assays and transmission electron microscopy (TEM) analysis were conducted with two different inoculation assays. The results indicated that the resistant leaves may have certain antifungal activity against A. alternate that is lacking in susceptible leaves. To elucidate the two different host responses to A. alternate infection in apples, the proteomes of susceptible and resistant apple leaves that had or had not been infected with pathogen were characterised using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS). MS identified 43 differentially expressed proteins in two different inoculation assays. The known proteins were categorised into 5 classes, among these proteins, some pathogenesis-related (PR) proteins, such as beta-1,3-glucanase, ascorbate peroxidase (APX), glutathione peroxidase (GPX) and mal d1, were identified in susceptible and resistant hosts and were associated with disease resistance of the apple host. In addition, the different levels of mal d1 in susceptible and resistant hosts may contribute to the outstanding anti-disease properties of resistant leaves against A. alternate. Taken together, the resistance mechanisms of the apple host against A. alternate may be a result of the PR proteins and other defence-related proteins. Given the complexity of the biology involved in the interaction between apple leaves and the A. alternate pathogen, further investigation will yield more valuable insights into the molecular mechanisms of suppression of the A. alternate pathogen. Overall, we outline several novel insights into the response of apple leaves to pathogen attacks. These findings increase our knowledge of pathogen resistance mechanisms, and the data will also promote further investigation into the regulation of the expression of these target proteins.

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“…Induction of β-1,3-glucanase has been reported in various plants infected with fungal pathogens including tomato (Joosten and De Wit 1989), wheat (Kemp et al 1999), peach (Zemanek et al 2002), rubber tree (Thanseem et al 2005), apple (Venisse et al 2002), rucola (Gupta et al 2013), V. flexuosa (Ahn et al 2012), and strawberry (Fang et al 2012). It has been suggested that these compounds play multiple roles in plant defense response.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of β-1,3-Glucanase Transcripts Induced by Pathogens in the Leaves of Vitis flexuosa

Ahn

Kim

Yun

2014

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT The differential expression of β-1,3-glucanase genes in the leaves of Vitis flexuosa induced by fungal and bacterial pathogen infections was investigated. The nucleotide and deduced amino acid sequences of β-1,3-glucanase genes from the transcripts of V. flexuosa were compared. The percentage similarity of deduced amino acid ranged from 22.0% between VfGlu34359 and VfGlu48103 to 96.9% in VvGlu2735 and VvGlu48103. To demonstrate the differential expression pattern of β-1,3-glucanase genes, primers specific to unique regions in each gene were obtained by alignment of the sequences. Accumulation patterns of β-1,3-glucanase mRNAs in the leaves of V. flexuosa were induced differentially and were dependent on the pathogens used including Botrytis cinerea, Colletotrichum acutatum, Elsinoe ampelina, and Rhizobium vitis. This study provides useful information that will improve our understanding of grapevine defense responses to various pathogen attacks.

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Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae

Cited by 54 publications

References 73 publications

Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)

Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)

Proteome Analysis of Pathogen-Responsive Proteins from Apple Leaves Induced by the Alternaria Blotch Alternaria alternata

Differential Expression of β-1,3-Glucanase Transcripts Induced by Pathogens in the Leaves of Vitis flexuosa

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