Proteomic analysis of temperature stress-responsive proteins in Arabidopsis thaliana rosette leaves

Abstract

Plants, as sessile organisms, are continuously exposed to temperature changes in the environment. Low and high temperature stresses have a great impact on agricultural productivity, since they significantly alter plant metabolism and physiology. Plant response to temperature stress is a quantitative character, being influenced by the degree of stress, time of exposure, as well as plant adaptation ability; it involves profound cellular changes at the proteomic level. We describe here the quantitative variations of the protein repertoire of Arabidopsis thaliana rosette leaves after exposing seedlings to either short-term cold or heat temperature stress. A proteomic approach, based on two-dimensional electrophoresis and MALDI-TOF peptide mass fingerprinting and/or nanoLC-ESI-LIT-MS/MS experiments, was used for this purpose. The comparison of the resulting proteomic maps highlighted proteins showing quantitative variations induced by temperature treatments. Thirty-eight protein spots exhibited significant quantitative changes under at least one stress condition. Identified, differentially-represented proteins belong to two main broad functional groups, namely energy production/carbon metabolism and response to abiotic and oxidative stresses. The role of the identified proteins is discussed here in relation to plant adaptation to cold or heat stresses. Our results suggest a significant overlapping of the responses to opposite temperature extremes.

Plant defense in response to chewing insects: proteome analysis ofArabidopsis thalianadamaged byPlutella xylostella

“…Similar observations have been reported with herbivorous insects or temperature stress in Arabidopsis plants (Collins et al 2010;Rocco et al 2013). Photosynthesis-related proteins (RuBis carboxylase large chain, oxygen-evolving enhancer protein 1-1, chloroplastic, oxygen-evolving enhancer protein 2-1, chloroplastic, and RuBis carboxylase) were observed in Table 1.…”

“…Moreover, it is indicated that heat stress causes downregulation of different key enzymes of the Calvin cycle in rice, including RuBis carboxylase (Han et al 2009). The expression of RuBis carboxylase large chain was significantly downregulated in Arabidopsis leaves due to heat treatment (Rocco et al 2013). Zou et al (2011) indicated that RuBis carboxylase could be considered as a part of the plant adaptive response, to maintain CO 2 fixation under stress factors.…”

“…For carbohydrate and energy metabolism, herbivorous insect and heat stress negatively influence the glycolytic pathway and decrease energy production in Arabidopsis plants (Liu et al 2010;Rocco et al 2013). In agreement with this scenario, we obtained a down-representation of sedoheptulose-1,7-bisphosphatase, carbonic anhydrase, chloroplastic, ATP synthase subunit beta, chloroplastic, and ATP synthase beta subunit, partial (chloroplast) in Arabidopsis leaves attacked by larvae.…”

“…The protein expression changes in A. thaliana leaves due to P. xylostella infestation were identified by two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry in several studies (Collins et al 2010;Liu et al 2010). However, such studies were only conducted at the optimum temperature for Arabidopsis growth (22°C) (Herde et al 2008;Antoun and Ouellet 2013), even if changes in temperature in the environment can lead to altered protein profiles in plants (Amme et al 2006;Loreto and Schnitzler 2010;Rocco et al 2013;ČErnÝ et al 2014). Therefore, here we aimed to detect changes in the proteins expressed in Arabidopsis leaves infested by larvae at a temperature higher than 22°C…”

Comparison of nano and conventional liquid chromatographic methods for the separation of (+)-catechin-ethyl-malvidin-3-glucoside diastereoisomers

“…As an example of successful application in plant research, a nanoLC-ESI-MS/MS analysis of proteins in excised spots from gel after two-dimensional electrophoretic purification and digestion of Arabidopsis thaliana leaves (i.e. analysis of small pieces of material) has been published [23].…”