Proteomic Analysis of the Antibacterial Effect of Improved Dian Dao San against Propionibacterium acnes

Ren, Shuaijun; Wang, Wenjiao; Jia, Min; An, Lili; Tang, Ting; Liu, Xin

doi:10.1155/2022/3855702

Cited by 2 publications

(2 citation statements)

References 33 publications

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“… 24 Our preliminary investigation revealed that the MIC of IDDS against C. acnes was 62.5mg/mL. 17 However, its potential antibacterial effect against C. acnes remains uncertain. So MBC and time-kill curves were explored.…”

Section: Discussionmentioning

confidence: 99%

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Study on Antibacterial Activity and Mechanism of Improved Dian Dao San Against Cutibacterium acnes (C. acnes)

An¹,

Gong²,

Hu³

et al. 2023

IDR

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The hyperproliferation of C. acnes has long been regarded as a primary etiological factor in the development of acne vulgaris (AV). Antibiotics targeting C. acnes have been the mainstay in AV treatment. Meanwhile, C. acnes has developed resistance to numerous antibiotics. IDDS, as traditional Chinese medicine, exhibits potent antibacterial activity against C. acnes. However, the mechanism of IDDS against C. acnes remains unclear. Methods: In this study, we conducted a systematic investigation in vitro to determine the minimal bactericidal concentration (MBC) and time-kill curves. The MBC and time-kill curves were assessed by quantifying Colony Forming Units countsIn order to establish an in vivo rat ear model of acne, a single intradermal injection of 100μL C. acnes suspension was administered, and oleic acid was applied to the right ear pinna for a duration of 14 days. The intervention involved the utilization of IDDS medications. Additionally, the levels of inflammatory mediators tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) were assessed using respective ELISA kits, while Hematoxylin and eosin (HE) staining was employed to visualize the rat ear model. The antimicrobial mechanism was investigated through the analysis of mRNA levels using real-time, quantitative PCR. ELISA analysis was performed according to the protocols outlined for energy metabolism and antioxidant system. Results: Our research has demonstrated that IDDS possesses antibacterial activity against C. acnes both in vitro and in vivo. The mechanisms underlying these effects involve energy metabolism and antioxidant systems. Conclusion:The data has provided further insights into the mechanism of IDDS against C. acnes, which establishes a robust foundation for the clinical application of IDDS.

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Section: Discussionmentioning

confidence: 99%

“…In our previous research, IDDS exhibited superior antibacterial effect against C. acnes . 17 However, the antibacterial activity of IDDS and its potential mechanisms have yet to be thoroughly investigated.…”

Section: Introductionmentioning

confidence: 99%

Study on Antibacterial Activity and Mechanism of Improved Dian Dao San Against Cutibacterium acnes (C. acnes)

An¹,

Gong²,

Hu³

et al. 2023

IDR

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DegS regulates the aerobic metabolism of Vibrio cholerae via the ArcA-isocitrate dehydrogenase pathway for growth and intestinal colonization

Zhao,

Huang,

et al. 2024

Front. Cell. Infect. Microbiol.

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Aerobic respiration is the key driver of Vibrio cholerae proliferation and infection. Our previous transcriptome results suggested that degS knockout downregulates a few genes involved in NADH and ATP synthesis in the aerobic respiratory pathway. In this study, non-targeted metabolomics results showed that the differential metabolites affected by degS knockout were associated with aerobic respiration. Further results suggested that the key products of aerobic respiration, NADH and ATP, were reduced upon degS deletion and were not dependent on the classical σE pathway. The two-component system response factor aerobic respiration control A (ArcA) is involved in regulating NADH and ATP levels. qRT-PCR demonstrated that DegS negatively regulates the transcription of the arcA gene, which negatively regulates the expression of isocitrate dehydrogenase (ICDH), a key rate-limiting enzyme of the tricarboxylic acid cycle. NADH and ATP levels were partially restored with the knockout of the arcA gene in the ΔdegS strain, while levels were partially restored with overexpression of ICDH in the ΔdegS strain. In a growth experiment, compared to the ΔdegS strain, the growth rates of ΔdegSΔarcA and ΔdegS-overexpressed icdh strains (ΔdegS+icdh) were partially restored during the logarithmic growth period. Colonization of the intestines of suckling mice showed a significant reduction in the colonizing ability of the ΔdegS strain, similar colonizing ability of the ΔdegS::degS strain and the wild-type strain, and a partial recovery of the colonizing ability of the ΔdegS+icdh strain. Overall, these findings suggest that the DegS protease regulates the expression of ICDH through ArcA, thereby affecting the NADH and ATP levels of V. cholerae and its growth and intestinal colonization ability.

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Proteomic Analysis of the Antibacterial Effect of Improved Dian Dao San against Propionibacterium acnes

Cited by 2 publications

References 33 publications

Study on Antibacterial Activity and Mechanism of Improved Dian Dao San Against Cutibacterium acnes (C. acnes)

Study on Antibacterial Activity and Mechanism of Improved Dian Dao San Against Cutibacterium acnes (C. acnes)

DegS regulates the aerobic metabolism of Vibrio cholerae via the ArcA-isocitrate dehydrogenase pathway for growth and intestinal colonization

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