Proteomic Analysis of the Function of Sigma Factor σ54 in Helicobacter pylori Survival with Nutrition Deficiency Stress In Vitro

Sun, Yuan; Liu, Shuang; Li, Wen; Shan, Yuqun; Li, Xinpeng; Lu, Xingxiao; Li, Yán; Guo, Qing; Zhou, Yabin; Jia, Jihui

doi:10.1371/journal.pone.0072920

Cited by 10 publications

(9 citation statements)

References 36 publications

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“…This is in agreement with studies conducted in Italy [21]. The σ 54 (rpoN) is known to controls several bacterial regulatory processes involved in energy metabolism, biosynthesis, oxidative stress and virulence [38]. σ 28 (fliA) has been reported to regulates the expression of outer membrane proteins, lipopolysaccharide synthesis, DNA restriction and cagA expression as well as control of LPXC gene which is involved in the early steps of lipid A synthesis of H. pylori [39][40][41].…”

Section: Discussionsupporting

confidence: 88%

Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria

Jolaiya

Fowora

Onyekwere

et al. 2020

J Infect Dev Ctries

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Introduction: Helicobacter pylori is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (dupA), the plasticity region genes and sigma factors in relation to their pathological expression of H. pylori infections in the Nigerian population. Methodology: Polymerase Chain Reaction was used to analyze a total of forty-nine H. pylori strains isolated from patients attending various endoscopic units in tertiary hospitals in Nigeria for complete dupA (G27 variant), jhp0917, jhp0918, other plasticity region genes jhp 914/917, jhp0914, jhp0940 and sigma factors. Results: PCR results indicated that the prevalence of complete dupA (G27 variants), jhp0917, jhp0918 and other plasticity region genes jhp0914, jhp0914/0917 and jhp0940 in the H. pylori strains were 4%, 53%, 88%, 73%, 12% and 0% respectively. The prevalence values of the sigma factors were 96%, 92%, 80% for rpoN, fliA and rpoD respectively. However, the endoscopic findings showed that erosion, normal mucosal, ulcer, hyperaemic stomach, mucosal atrophy and oedematous stomach in the patients where the H. pylori strains were isolated were 40.8%, 32.7%, 10.2%, 8.2%, 2.0% and 6.1% respectively. There was significant association between jhp0917, jhp914/917 and G27 variant and the endoscopic findings, while other plasticity genes showed no association with the endoscopic findings. Conclusion: These results suggest that the presence of jhp0917, jhp0914/917 and G27 variant could be used as marker to predict the pathological effect of severity in Nigeria patients with H. pylori infection.

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Section: Discussionsupporting

confidence: 88%

Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria

Jolaiya

Fowora

Onyekwere

et al. 2020

J Infect Dev Ctries

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“…Whereas all of the above conditions boosted fliA expression, rpoD showed only a mild increase in transcription after bacterial exposure to acid pH, urea, and nickel, and a decrease in response to iron. The expression of rpoN significantly increased upon treatment of bacteria with urea or nickel; RpoN was initially described as regulator of flagellar genes, but recent studies show that it also controls several bacterial regulatory processes involved in energy metabolism, biosynthesis, protein fate, oxidative stress, and virulence (Sun et al, 2013). fliA was strongly expressed even under environmental conditions that are not common inducers of flagella synthesis, but are known to affect other bacterial components or pathways.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of Transcription Factors of Helicobacter pylori under Different Environmental Conditions

et al. 2017

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Helicobacter pylori is a Gram-negative bacterium that colonizes the human gastric mucosa and causes peptic ulcers and gastric carcinoma. H. pylori strain 26695 has a small genome (1.67 Mb), which codes for few known transcriptional regulators that control bacterial metabolism and virulence. We analyzed by qRT-PCR the expression of 16 transcriptional regulators in H. pylori 26695, including the three sigma factors under different environmental conditions. When bacteria were exposed to acidic pH, urea, nickel, or iron, the sigma factors were differentially expressed with a particularly strong induction of fliA. The regulatory genes hrcA, hup, and crdR were highly induced in the presence of urea, nickel, and iron. In terms of biofilm formation fliA, flgR, hp1021, fur, nikR, and crdR were induced in sessile bacteria. Transcriptional expression levels of rpoD, flgR, hspR, hp1043, and cheY were increased in contact with AGS epithelial cells. Kanamycin, chloramphenicol, and tetracycline increased or decreased expression of regulatory genes, showing that these antibiotics affect the transcription of H. pylori. Our data indicate that environmental cues which may be present in the human stomach modulate H. pylori transcription.

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“…To detect the NapA and CagA protein expressions with or without IFN- γ treatment, we collected the liquid bacterial culture of H. pylori 26695 and 26695 Δomp18 isogenic mutants at 8 h. Bacterial cell lysates were prepared as described [ 25 ]. To determine phosphotyrosine STAT1 protein expression, we seeded macrophages at 2 × 10 6 /well in flat-bottomed 6-well microplates for 24 h and then infected them with H. pylori 26695 with or without IFN- γ (50 pg/mL) for 6 h. Cells were washed twice with ice-cold phosphate buffered saline (PBS) and lysed in RIPA buffer (Beyotime Biotechnology, China) with 1% PMSF.…”

Section: Methodsmentioning

confidence: 99%

“…Approximately 25–30 μ g protein for each sample was loaded and separated by SDS-PAGE. They were then probed with specific antibodies against NapA (obtained from our lab) [ 25 ], STAT1 (Cell Signaling Technology, #5375), and CagA (Abcam, ab90490), followed by anti-mouse or rabbit horseradish peroxidase-conjugated IgG. They were then developed with the enhanced chemiluminescence method.…”

Section: Methodsmentioning

confidence: 99%

Helicobacter pyloriOuter Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γSignaling

Shan

Han

et al. 2015

BioMed Research International

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Outer membrane proteins (OMPs) can induce an immune response. Omp18 (HP1125) of H. pylori is a powerful antigen that can induce significant interferon-γ (IFN-γ) levels. Previous studies have suggested that IFN-γ plays an important role in H. pylori clearance. However, H. pylori has multiple mechanisms to avoid host immune surveillance for persistent colonization. We generated an omp18 mutant (H. pylori 26695 and H. pylori SS1) strain to examine whether Omp18 interacts with IFN-γ and is involved in H. pylori colonization. qRT-PCR revealed that IFN-γ induced Omp18 expression. qRT-PCR and western blot analysis revealed reduced expressions of virulence factors CagA and NapA in H. pylori 26695 with IFN-γ treatment, but they were induced in the Δomp18 strain. In C57BL/6 mice infected with H. pylori SS1 and the Δomp18 strain, the Δomp18 strain conferred defective colonization and activated a stronger inflammatory response. Signal transducer phosphorylation and transcription 1 (STAT1) activator was downregulated by the wild-type strain but not the Δomp18 strain in IFN-γ-treated macrophages. Furthermore, Δomp18 strain survival rates were poor in macrophages compared to the wild-type strain. We concluded that H. pylori Omp18 has an important function influencing IFN-γ-mediated immune response to participate in persistent colonization.

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Proteomic Analysis of the Function of Sigma Factor σ54 in Helicobacter pylori Survival with Nutrition Deficiency Stress In Vitro

Cited by 10 publications

References 36 publications

Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria

Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria

Gene Expression Profiling of Transcription Factors of Helicobacter pylori under Different Environmental Conditions

Helicobacter pyloriOuter Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γSignaling

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