Proteomic analysis of the impacts of powdery mildew on wheat grain

Li, Jie; Liu, Xinhao; Yang, Xin; Li, Yongchun; Wang, Chenyang; He, Dexian

doi:10.1016/j.foodchem.2018.04.024

Cited by 23 publications

(27 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The serpin gene family has gained attention in wheat and barley for its role in cereal grain development (Østergaard et al 2004; Roberts et al 2003; Roberts and Hejgaard 2008). In addition, serpins have also been implicated in the disease response in soybean, maize, Arabidopsis , tomato, barley and wheat (Solomon et al 1999; Erb et al 2009; Laluk and Mengiste 2011; Jashni et al 2015; Fluhr et al 2012; Bhattacharjee et al 2015; Bhattacharjee et al 2017; Pekkarinen et al 2007; Paper et al 2007; Mirzadi Gohari et al 2015; Lema Asqui et al 2018; Li et al 2018b). This is the first genome-wide characterization and expression analysis of this agronomically important gene family in hexaploid wheat.…”

Section: Discussionmentioning

confidence: 99%

Serpins: Genome-Wide Characterisation and Expression Analysis of the Serine Protease Inhibitor Family inTriticum aestivum

Benbow

Jermiin

Doohan

2019

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

The serine protease inhibitor (serpin) gene family is the largest family of protease inhibitors. Serine protease inhibitors have an active, but under-characterized, role in grain development and defense against pathogen attack in cereal crops. By exploiting publicly available genomic, transcriptomic and proteomic data for wheat ( Triticum aestivum ), we have identified and annotated the entire ’serpinome’ of wheat and constructed a high-quality and robust phylogenetic tree of the gene family, identifying paralogous and homeologous clades from the hexaploid wheat genome, including the Serpin-Z group that have been well characterized in barley. Using publicly available RNAseq data ( http://www.wheat-expression.com/ ), expression profiles of the wheat serpins were explored across a variety of tissues from the developing grain, spikelet and spike. We show that the SERPIN-Z clade, among others, are highly expressed during grain development, and that there is homeologous and paralogous functional redundancy in this gene family. Further to their role in grain development, serpins play an important but under-explored role in response to fungal pathogens. Using 13 RNAseq datasets of wheat tissues infected by fungal pathogens, we identified 37 serpins with a significant disease response. The majority of the disease-responsive serpins were upregulated by Fusarium graminearum , a destructive fungal pathogen that attacks the spike and developing grain of wheat. As serpins are ubiquitous in wheat grain, the genes encoding serpins may be linked to grain development, with their disease response a result of pleiotropy.

show abstract

Section: Discussionmentioning

confidence: 99%

Serpins: Genome-Wide Characterisation and Expression Analysis of the Serine Protease Inhibitor Family inTriticum aestivum

Benbow

Jermiin

Doohan

2019

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…Carbohydrates in the grains mainly come from photosynthesis during the grain filling (Blum, 1998). The other important storage compound of the wheat grain is protein-related with the nitrogen (N) dynamics and accounts for 10-15%, whose composition is essential for flour quality (Li et al, 2018). The deposition and redistribution of N are crucial processes regulating grain yield and grain quality in wheat (Gaju et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

How Foliar Fungal Diseases Affect Nitrogen Dynamics, Milling, and End-Use Quality of Wheat

et al. 2020

View full text Add to dashboard Cite

“…The impact of powdery mildew on grain quality, such as test weight and protein content, has been reported [2]. Changes in grain proteome and composition and grain starch and protein contents caused by the disease were observed using the proteomics analysis [3].…”

Section: Introductionmentioning

confidence: 99%

Development of SNP, KASP, and SSR Markers by BSR-Seq Technology for Saturation of Genetic Linkage Map and Efficient Detection of Wheat Powdery Mildew Resistance Gene Pm61

et al. 2019

IJMS

View full text Add to dashboard Cite

The gene Pm61 that confers powdery mildew resistance has been previously identified on chromosome arm 4AL in Chinese wheat landrace Xuxusanyuehuang (XXSYH). To facilitate the use of Pm61 in breeding practices, the bulked segregant analysis-RNA-Seq (BSR-Seq) analysis, in combination with the information on the Chinese Spring reference genome sequence, was performed in the F2:3 mapping population of XXSYH × Zhongzuo 9504. Two single nucleotide polymorphism (SNP), two Kompetitive Allele Specific PCR (KASP), and six simple sequence repeat (SSR) markers, together with previously identified polymorphic markers, saturated the genetic linkage map for Pm61, especially in the proximal side of the target gene that was short of gene-linked markers. In the newly established genetic linkage map, Pm61 was located in a 0.71 cM genetic interval and can be detected in a high throughput scale by the KASP markers Xicsk8 and Xicsk13 or by the standard PCR-based markers Xicscx497 and Xicsx538. The newly saturated genetic linkage map will be useful in molecular marker assisted-selection of Pm61 in breeding for disease resistant cultivar and in its map-based cloning.

show abstract

Proteomic analysis of the impacts of powdery mildew on wheat grain

Cited by 23 publications

References 29 publications

Serpins: Genome-Wide Characterisation and Expression Analysis of the Serine Protease Inhibitor Family inTriticum aestivum

Serpins: Genome-Wide Characterisation and Expression Analysis of the Serine Protease Inhibitor Family inTriticum aestivum

How Foliar Fungal Diseases Affect Nitrogen Dynamics, Milling, and End-Use Quality of Wheat

Development of SNP, KASP, and SSR Markers by BSR-Seq Technology for Saturation of Genetic Linkage Map and Efficient Detection of Wheat Powdery Mildew Resistance Gene Pm61

Contact Info

Product

Resources

About