Proteomic analysis of the pathophysiological process involved in the antisnake venom effect of Mucuna pruriens extract

Abstract

Previously, we reported the antisnake venom properties of a Mucuna pruriens seed extract (MPE) and tested its in vivo efficacy against Echis carinatus venom (EV) in short- (1 injection) and long-term (three weekly injections) treatments. The aim of the present study was to investigate plasma proteome changes associated with MPE treatments and identify proteins responsible for survival of envenomated mice (CHALLENGED mice). Six treatment groups were studied. Three control groups: one saline, one short-term and one long-term MPE treatment. One group received EV alone. Two test groups received EV with either a short-term or long-term MPE treatment (CHALLENGED mice). The plasma from each group was analysed by 2-DE/MALDI-TOF MS. The most significant changes with treatment were: albumin, haptoglobin, fibrinogen, serum amyloid A and serum amyloid P. Most of these changes were explained by EV effects on coagulation, inflammation and haemolysis. However, MPE treatments prevented the EV-induced elevation in HPT. Consequently, HPT levels were similar to controls in the plasma of CHALLENGED mice. The plasma of CHALLENGED mice showed substantial proteomic modifications. This suggests the mechanism of MPE protection involves the activation of counterbalancing processes to compensate for the imbalances caused by EV.

The protective effect of Mucuna pruriens seeds against snake venom poisoning

“…Proteomic analysis, for example, indicated that the MPE pre-treatment also elicited the activation of counterbalancing processes to compensate for the imbalances caused by Echis carinatus venom (Guerranti et al, 2008).…”

Effects of Mucuna pruriens Protease Inhibitors on Echis carinatus Venom

“…Mucuna pruriens and its glycoprotein extract were prepared as previously described in Guerranti et al . (). Eluted fractions were assayed for protein concentration by the dye‐binding method of Bradford () and purity checked with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE).…”

“…Mucuna pruriens has been studied extensively for its chemical, pharmacological (Iauk et al ., ; Katzenschlager et al ., ) and antisnake venom properties (Houghton and Osibogun, ; Ogueli et al ., ; Guerranti et al ., , ; Soares et al ., ). In a separate study by Machuka (), the total protein extract from M. pruriens seeds inhibited trypsin and chymotrypsin activities.…”

A novel serine protease from the snake venom of Agkistrodon blomhoffii ussurensis

“…High-throughput proteomic/transcriptomic snake venom analyses have proved the presence of various SPases in the venoms of crotalidae and viperidae snakes (Calvete et al, 2007; Fox et al, 2006; Guerranti et al, 2008; Li et al, 2004; Serrano et al, 2005). …”