Proteomic analysis of the venom from the scorpion Mesobuthus martensii

Xu, Xiaobo; Duan, Zhigui; Di, Zhiyong; He, Yawen; Li, Jianglin; Li, Zhongjie; Xie, Conghua; Zeng, Xin; Cao, Zhijian; Wu, Yingliang; Liang, Songping; Li, Wenxin

doi:10.1016/j.jprot.2014.04.032

Cited by 50 publications

(38 citation statements)

References 66 publications

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“…Mass spectrometry (MS) was performed as described previously [18,40]. Venom peptides were reduced with dithiothreitol (DTT) and alkylated with iodoacetamide (IAA).…”

Section: Proteomics Analysismentioning

confidence: 99%

Unique diversity of the venom peptides from the scorpion Androctonus bicolor revealed by transcriptomic and proteomic analysis

Zhang

Shi

Zeng

et al. 2015

Journal of Proteomics

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“…Mass spectrometry (MS) was performed as described previously [18,40]. Venom peptides were reduced with dithiothreitol (DTT) and alkylated with iodoacetamide (IAA).…”

Section: Proteomics Analysismentioning

confidence: 99%

Unique diversity of the venom peptides from the scorpion Androctonus bicolor revealed by transcriptomic and proteomic analysis

Zhang

Shi

Zeng

et al. 2015

Journal of Proteomics

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“…Proteomic analysis of the scorpion Mesobuthus martensii has been reported before and proteins from this species were isolated by 2-DE, SDS-PAGE, and RP-HPLC [8]. In order to focus on peptides, fractions with mass weight less than 10 KD (identified by MALDI-TOF MS) were analyzed to uncover the complexity of the venom.…”

Section: Resultsmentioning

confidence: 99%

“…The combined methods (proteomic and transcriptomic) have been used to unravel the diversity of scorpion venoms. Complex components of Chinese scorpion venom, such as Mesobuthus martensii (also known as Buthus Martensi Karsch ) [7], Androctonus bicolor , and Heterometrus petersii , were revealed by chromatography (2-DE, SDS-PAGE, and RP-HPLC) with mass spectrometry (ESI-Q-TOF MS and MALDI-TOF MS), and transcriptomics [8,9,10,11]. Two-hundred twenty-seven proteins unambiguously have been identified from M. martensii .…”

Section: Introductionmentioning

confidence: 99%

A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii

Luan

Wang

Liu

et al. 2016

Toxins

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Scorpion venom is deemed to contain many toxic peptides as an important source of natural compounds. Out of the two hundred proteins identified in Mesobuthus martensii (M. martensii), only a few peptide toxins have been found so far. Herein, a combinational approach based upon RNA sequencing and Liquid chromatography-mass spectrometry/mass spectrometry (LC MS/MS) was employed to explore the venom peptides in M. martensii. A total of 153 proteins were identified from the scorpion venom, 26 previously known and 127 newly identified. Of the novel toxins, 97 proteins exhibited sequence similarities to known toxins, and 30 were never reported. Combining peptidomic and transcriptomic analyses, the peptide sequence of BmKKx1 was reannotated and four disulfide bridges were confirmed within it. In light of the comparison of conservation and variety of toxin amino acid sequences, highly conserved and variable regions were perceived in 24 toxins that were parts of two sodium channel and two potassium channel toxins families. Taking all of this evidences together, the peptidomic analysis on M. martensii indeed identified numerous novel scorpion peptides, expanded our knowledge towards the venom diversity, and afforded a set of pharmaceutical candidates.

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“…Venom comprises a mixture of a variety of biologically active components: enzymes, peptides, nucleotides, lipids, mucoproteins and biogenic amines that most of which have not been investigated yet [7][8][9][10][11]. Scorpion sting can cause a broad range of condition from sever local skin pain to multisystem failure followed by death [12]; despite that, scorpions and their venom has been used for centuries as medical treatments in traditional medicine in India, China, Africa and Cuba [13].…”

Section: Introductionmentioning

confidence: 99%