Unique diversity of the venom peptides from the scorpion Androctonus bicolor revealed by transcriptomic and proteomic analysis

Zhang, Lei; Shi, Wanxia; Zeng, Xian‐Chun; Ge, Feng; Yang, Mingkun; Nie, Yao; Bao, Aorigele; Wu, Shifen; Guoji, E

doi:10.1016/j.jprot.2015.07.030

Cited by 61 publications

(38 citation statements)

References 80 publications

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“…We found four sequences with identities ranging from 42% to 46% with the precursor of venom insulin-like growth factor binding protein 1 deduced from cDNA cloned from Mesobuthus martensii (only submitted to GenBank); three sequences with 44%–51% identity with the precursor of Tbah01400 deduced from the transcriptome analysis of the venom gland of Tityus bahiensis [41]; one sequence with 28% identity with the precursor of venom protein 29 and one sequence with 45% identity with the precursor of venom protein 302, both deduced from the transcriptome analysis of the venom gland of Lychas mucronatus [32]. We also found one sequence with 44% identity with the precursor of the orphan peptide AbOp5 deduced from the transcriptome analysis of Androctonus bicolor [66]; and one sequence with 49% identity with the precursor of Tbah02469 deduced from the transcriptome analysis of T. bahiensis [41]. Finally, two components had hits with 25% identity (both) with the precursor of venom allergen 5 deduced from the genome analysis of the spider Stegodyphus mimosaroum (only submitted to GenBank), and one component had hits with 55% identity with the precursor of putative scp tpx 1 ag5 pr1 deduced from the transcriptome analysis of the mite Ixodes ricinus [67].…”

Section: Resultsmentioning

confidence: 99%

Venom Gland Transcriptomic and Proteomic Analyses of the Enigmatic Scorpion Superstitionia donensis (Scorpiones: Superstitioniidae), with Insights on the Evolution of Its Venom Components

Santibáñez‐López

Cid-Uribe

Batista

et al. 2016

Toxins

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Venom gland transcriptomic and proteomic analyses have improved our knowledge on the diversity of the heterogeneous components present in scorpion venoms. However, most of these studies have focused on species from the family Buthidae. To gain insights into the molecular diversity of the venom components of scorpions belonging to the family Superstitioniidae, one of the neglected scorpion families, we performed a transcriptomic and proteomic analyses for the species Superstitionia donensis. The total mRNA extracted from the venom glands of two specimens was subjected to massive sequencing by the Illumina protocol, and a total of 219,073 transcripts were generated. We annotated 135 transcripts putatively coding for peptides with identity to known venom components available from different protein databases. Fresh venom collected by electrostimulation was analyzed by LC-MS/MS allowing the identification of 26 distinct components with sequences matching counterparts from the transcriptomic analysis. In addition, the phylogenetic affinities of the found putative calcins, scorpines, La1-like peptides and potassium channel κ toxins were analyzed. The first three components are often reported as ubiquitous in the venom of different families of scorpions. Our results suggest that, at least calcins and scorpines, could be used as molecular markers in phylogenetic studies of scorpion venoms.

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Section: Resultsmentioning

confidence: 99%

Venom Gland Transcriptomic and Proteomic Analyses of the Enigmatic Scorpion Superstitionia donensis (Scorpiones: Superstitioniidae), with Insights on the Evolution of Its Venom Components

Santibáñez‐López

Cid-Uribe

Batista

et al. 2016

Toxins

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“…Other annotated transcripts listed in Table 1 function in translation (e.g., Elongation factor 1-alpha, 60s ribosomal protein I32), cytoskeletal (e.g., Actin 57B), and cellular or inflammatory processes (e.g., cytochrome C oxidase S3, ferritin, bradykinin-potentiating peptide). Several highly expressed transcripts, such as bradykinin-potentiating peptide and metalloserrulase, are well described in other scorpion species [ 46 – 48 ]. Eight uncharacterized/ ‘hypothetical' proteins were also identified and potentially represent new venom components…”

Section: Resultsmentioning

confidence: 99%

Differential toxicity and venom gland gene expression in Centruroides vittatus

et al. 2017

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Variation in venom toxicity and composition exists in many species. In this study, venom potency and venom gland gene expression was evaluated in Centruroides vittatus, size class I-II (immature) and size class IV (adults/penultimate instars) size classes. Venom toxicity was evaluated by probit analysis and returned ED50 values of 50.1 μg/g for class IV compared to 134.2 μg/g for class I-II 24 hours post injection, suggesting size class IV was 2.7 fold more potent. Next generation sequencing (NGS and qPCR were used to characterize venom gland gene expression. NGS data was assembled into 36,795 contigs, and annotated using BLASTx with UNIPROT. EdgeR analysis of the sequences showed statistically significant differential expression in transcripts associated with sodium and potassium channel modulation. Sodium channel modulator expression generally favored size class IV; in contrast, potassium channel modulators were favored in size class I-II expression. Real-time quantitative PCR of 14 venom toxin transcripts detected relative expression ratios that paralleled NGS data and identified potential family members or splice variants for several sodium channel modulators. Our data suggests ontogenetic differences in venom potency and venom related genes expression exist between size classes I-II and IV.

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“…The chief function of this compound is to induce akinesia and anxiety-like symptoms in mice. [ 10 11 ] However, the anticancer property of AB has been not yet examined. The objective of the present study is to find the anticancer potential of AB venom.…”

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confidence: 99%

Scorpion (Androctonus bicolor) venom exhibits cytotoxicity and induces cell cycle arrest and apoptosis in breast and colorectal cancer cell lines

et al. 2016

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Objectives:The defective apoptosis is believed to play a major role in the survival and proliferation of neoplastic cells. Hence, the induction of apoptosis in cancer cells is one of the targets for cancer treatment. Researchers are considering scorpion venom as a potent natural source for cancer treatment because it contains many bioactive compounds. The main objective of the current study is to evaluate the anticancer property of Androctonus bicolor scorpion venom on cancer cells.Materials and Methods:Scorpions were milked by electrical stimulation of telsons and lyophilized. The breast (MDA-MB-231) and colorectal (HCT-8) cancer cells were maintained in appropriate condition. The venom cytotoxicity was assessed by 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, and the cellular and nuclear changes were studied with propidium iodide and 4’,6-diamidino-2-phenylindole stain, respectively. The cell cycle arrest was examined using muse cell analyzer.Results:The A. bicolor venom exerted cytotoxic effects on MDA-MB-231 and HCT-8 cells in a dose- and duration-dependent manner and induced apoptotic cell death. The treatment with this venom arrests the cancer cells in G0/G1 phase of cell cycle.Conclusions:The venom selectively induces the rate of apoptosis in MDA-MB-231 and HCT-8 cells as reflected by morphological and cell cycle studies. To the best of our knowledge, this is the first scientific evidence demonstrating the induction of apoptosis and cell cycle arrest by A. bicolor scorpion venom.

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Unique diversity of the venom peptides from the scorpion Androctonus bicolor revealed by transcriptomic and proteomic analysis

Cited by 61 publications

References 80 publications

Venom Gland Transcriptomic and Proteomic Analyses of the Enigmatic Scorpion Superstitionia donensis (Scorpiones: Superstitioniidae), with Insights on the Evolution of Its Venom Components

Venom Gland Transcriptomic and Proteomic Analyses of the Enigmatic Scorpion Superstitionia donensis (Scorpiones: Superstitioniidae), with Insights on the Evolution of Its Venom Components

Differential toxicity and venom gland gene expression in Centruroides vittatus

Scorpion (Androctonus bicolor) venom exhibits cytotoxicity and induces cell cycle arrest and apoptosis in breast and colorectal cancer cell lines

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