2011
DOI: 10.1002/pmic.201100477
|View full text |Cite
|
Sign up to set email alerts
|

Proteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT)

Abstract: Exosomes are membrane vesicles that are secreted by cells upon fusion of multivesicular bodies with the plasma membrane. Exosomal proteomics has emerged as a powerful approach to understand the molecular composition of exosomes and has potential to accelerate biomarker discovery. Different proteomic analysis methods have been previously employed to establish several exosome protein databases. In this study, TFE solution phase digestion was compared with in-gel digestion and found to yield similar results. Prot… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

5
174
0

Year Published

2013
2013
2022
2022

Publication Types

Select...
10

Relationship

1
9

Authors

Journals

citations
Cited by 157 publications
(179 citation statements)
references
References 41 publications
5
174
0
Order By: Relevance
“…Exosomes contain lipids, proteins, and nucleic acids (mRNAs and miRNAs) 2 (5,6). The proteomic composition of exosomes has been well characterized (7)(8)(9)(10). Exosomes released into the intercellular space can fuse with multiple target cells and exert regulatory influences on the target cell (11)(12)(13)(14)(15).…”
Section: -10mentioning
confidence: 99%
“…Exosomes contain lipids, proteins, and nucleic acids (mRNAs and miRNAs) 2 (5,6). The proteomic composition of exosomes has been well characterized (7)(8)(9)(10). Exosomes released into the intercellular space can fuse with multiple target cells and exert regulatory influences on the target cell (11)(12)(13)(14)(15).…”
Section: -10mentioning
confidence: 99%
“…5 In the present study, we tested the hypothesis that RAAS activation during a lowsodium (LS) diet and during exogenous aldosterone infusion alters the urinary exosome proteomic profile. We extended these findings using a more targeted, sensitive, and quantitative approach to investigate the profile of ENaC and NCC, which are known to play an essential role in renal sodium and potassium homeostasis.…”
mentioning
confidence: 99%
“…The findings presented here are novel for gal-3 and gal-8, since no literature data regarding their presence in EVs of trophoblast origin have been reported so far. However, both gal-3 and gal-8 were found in EVs from different cell types or body fluids (Ogawa et al, 2008;Wang et al, 2012;Webber et al, 2014;Hurwitz et al, 2016).…”
Section: Discussionmentioning
confidence: 99%