Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity

Abstract

The impact of water deficit and salt stress on two important wine grape cultivars, Chardonnay and Cabernet Sauvignon, was investigated. Plants were exposed to increasing salinity and water deficit stress over a 16 d time period. Measurements of stem water potentials, and shoot and leaf lengths indicated that Chardonnay was more tolerant to these stresses than Cabernet Sauvignon. Shoot tips were harvested every 8 d for proteomic analysis using a trichloroacetic acid/acetone extraction protocol and two-dimensional gel electrophoresis. Proteins were stained with Coomassie Brilliant Blue, quantified, and then 191 unique proteins were identified using matrix-assisted laser desorption ionization time of flight/time of flight mass spectrometry. Peptide sequences were matched against both the NCBI nr and TIGR Vitis expressed sequence tag (EST) databases that had been implemented with all public Vitis sequences. Approximately 44% of the protein isoforms could be identified. Analysis of variance indicated that varietal difference was the main source of protein expression variation (40%). In stressed plants, reduction of the amount of proteins involved with photosynthesis, protein synthesis, and protein destination was correlated with the inhibition of shoot elongation. Many of the proteins up-regulated in Chardonnay were of unclassified or of unknown function, whereas proteins specifically up-regulated in Cabernet Sauvignon were involved in protein metabolism.

Proteome Analysis of Detached Fronds from a Resurrection Plant Selaginella Bryopteris - Response to Dehydration and Rehydration

“…Two ribosomal proteins, 40S RPS27 and 60S RPL27, were up regulated under desiccation stress (Table 1, Figure 5). Vincent et al (2007) have reported that in grapevine shoots, there is an increased abundance of RPL39 in response to drought. In yeast, this protein is a 60S ribosomal subunit implicated in translational accuracy (Dresios et al, 2000).…”

Overexpression of Arabidopsis Translationally Controlled Tumor Protein Gene AtTCTP Enhances Drought Tolerance with Rapid ABA-Induced Stomatal Closure

“…Thus, we investigated other phenotypes than growth. Based on the other previous studies that suggested the involvement of plant TCTP in abiotic stress signaling such as water deficit (Vincent et al, 2007), we investigated drought stress responses and found significantly enhanced drought tolerance in the AtTCTP overexpression plants (Fig. 1).…”

“…Since it has been reported that plant TCTP shows increased expressional levels in response to water and osmotic stress (Ermolayev et al, 2003;Vincent et al, 2007), we performed the expression analysis of AtTCTP in the presence of ABA using the transgenic plants carrying AtTCTP pro :GUS construct. The results showed that GUS expression was detected in whole roots of transgenic seedlings with AtTCTP pro :GUS in the presence of ABA, whereas that was detected only in root apical meristem in the absence of ABA (Supplementary Fig.…”

“…However, other studies suggest that the plant TCTP is involved in abiotic stress signaling such as aluminum, salt, and water deficit by transcriptomic or proteomic analyses (Ermolayev et al, 2003;Vincent et al, 2007). For examples, the expression level of plant TCTP increased in response to cold, salt, and heavy metal treatment (Ermolayev et al, 2003;Vincent et al, 2007), and decreased in response to pathogen infection (Ascencio-Ibanez et al, 2008;Fabro et al, 2008;Jones et al, 2006). Therefore, the biological function of plant TCTPs must be further investigated.…”

Mild osmotic stress in intertidal gastropods Littorina saxatilis and Littorina obtusata (Mollusca: Caenogastropoda): a proteomic analysis

“…TCTP is also an important participant of the TOR-pathway, a nutrition sensor and growth regulator in different organisms (Deprost et al, 2007;Brioudes et al, 2010). Its regulation was detected during salinity stress in plants and bacteria (Vincent et al, 2007;Santa Brígida et al, 2014). TCTP proved to be down-regulated during mild salinity stress in both Littorina species.…”