Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells

Cheng, Lin; Zhang, Kun; Qing, Yun’an; Dong, Li; Cui, Manhua; Jin, Peng; Xu, Tianmin

doi:10.1186/s13048-020-0609-y

Cited by 71 publications

(65 citation statements)

References 47 publications

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“…These factors are either related to tumor development or metastasis. Noticeably, a very recent study showed the higher concentration of lipoprotein lipase (LPL) and collagen type V alpha 2 chain (COL5A2) in exosomes derived from ovarian cancer cells (SKOV-3) compared to ovarian surface epithelial cells (HOSEPiC) by proteomic and lipidomic analysis [ 171 ]. Furthermore, exosomal proteins might be involved in drug resistance.…”

Section: Exosomes In the Pathophysiology Of Female Reproductionmentioning

confidence: 99%

Exosomes as Biomarkers for Female Reproductive Diseases Diagnosis and Therapy

Esfandyari

Elkafas

Chugh

et al. 2021

IJMS

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Cell–cell communication is an essential mechanism for the maintenance and development of various organs, including the female reproductive system. Today, it is well-known that the function of the female reproductive system and successful pregnancy are related to appropriate follicular growth, oogenesis, implantation, embryo development, and proper fertilization, dependent on the main regulators of cellular crosstalk, exosomes. During exosome synthesis, selective packaging of different factors into these vesicles happens within the originating cells. Therefore, exosomes contain both genetic and proteomic data that could be applied as biomarkers or therapeutic targets in pregnancy-associated disorders or placental functions. In this context, the present review aims to compile information about the potential exosomes with key molecular cargos that are dysregulated in female reproductive diseases which lead to infertility, including polycystic ovary syndrome (PCOS), premature ovarian failure (POF), Asherman syndrome, endometriosis, endometrial cancer, cervical cancer, ovarian cancer, and preeclampsia, as well as signaling pathways related to the regulation of the reproductive system and pregnancy outcome during these pathological conditions. This review might help us realize the etiology of reproductive dysfunction and improve the early diagnosis and treatment of the related complications.

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Section: Exosomes In the Pathophysiology Of Female Reproductionmentioning

confidence: 99%

Exosomes as Biomarkers for Female Reproductive Diseases Diagnosis and Therapy

Esfandyari

Elkafas

Chugh

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The lipid composition of EVs can be studied in detail using mass spectrometry, high-performance liquid chromatography, nuclear magnetic resonance, gas chromatography-mass spectrometry, and computational approaches [ 56 – 62 ]. One study identified differences in lipid composition between EVs of ovarian cancer cells and ovarian epithelial cells, and these differences may facilitate early diagnosis [ 63 ]. Another study found that analysis of exosomal lipids was able to distinguish non-small-cell lung cancer in the early or late stages [ 64 ].…”

Section: Biogenesis and Contents Of Evsmentioning

confidence: 99%

Extracellular Vesicles in Liquid Biopsies: Potential for Disease Diagnosis

Liu

Chen

Pei

et al. 2021

BioMed Research International

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Liquid biopsy is conducted through minimally invasive or noninvasive procedures, and the resulting material can be subjected to genomic, proteomic, and lipidomic analyses for early diagnosis of cancers and other diseases. Extracellular vesicles (EVs), one kind of promising tool for liquid biopsy, are nanosized bilayer particles that are secreted by all kinds of cells and that carry cargoes such as lipids, proteins, and nucleic acids, protecting them from enzymatic degradation in the extracellular environment. In this review, we provide a comprehensive introduction to the properties and applications of EVs, including their biogenesis, contents, sample collection, isolation, and applications in diagnostics based on liquid biopsy.

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“…They identified 1433 proteins and 1227 lipids in the exosomes derived from the two cell lines and observed significant differences in several lipids and proteins in the two exosomes populations. Their study implied combination of proteomic and lipidomic profiles could be a promising approach in the early diagnosis of ovarian cancer ( 49 ). Application of such unique approaches make exosomes excellent candidates for developing biomarkers and strategies for liquid biopsies.…”

Section: Content Of Evs For Immunomodulation and As Candidates Of Liqmentioning

confidence: 99%

Extracellular Vesicles in Oncology: from Immune Suppression to Immunotherapy

Srivastava

Rathore

Munshi

et al. 2021

AAPS J

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Exosomes are involved in cell-to-cell communication and play a crucial role in cellular physiology. The role of exosomes in cancer has been widely explored. Tumor cells have evolved and adapted to evade the immune response. The study of the immune system’s modulations in favor of rogue tumor cells led to the development of a novel immunotherapeutic strategy targeting the immune checkpoint proteins (ICPs). In clinical settings, the response to ICP therapy has been inconsistent and is difficult to predict. Quantitating the targeted ICPs through immunohistochemistry is one approach, but is not pragmatic in a clinical setting and is often not sensitive. Examining the molecules present in bodily fluids to determine ICP treatment response, “liquid biopsy” is a convenient alternative. The term “liquid biopsy” refers to circulating tumor cells (CTCs), extracellular vesicles (EVs), non-coding (nc) RNA, circulating tumor DNA (ctDNA), circulating free DNA (cfDNA), etc. EVs includes exosomes, microvesicles, and oncosomes. Herein, we focus on exosomes isolated from bodily fluids and their use in liquid biopsy. Due to their unique ability to transfer bioactive molecules and perturb the physiology of recipient cells, exosomes have garnered attention for their immune modulation role and as a resource to identify molecules associated with liquid biopsy–based diagnostic methods. In this review, we examine the putative role of exosomes and their cargo in influencing the immune system. We discuss the immune and tumor cells present in the tumor microenvironment (TME), and the exosomes derived from these cells to understand how they participate in creating the immune-suppressive TME. Additionally, use of exosomes in liquid biopsy–based methods to measure the treatment response elicited by immunotherapy is discussed. Finally, we describe how exosomes have been used to develop immune therapies, especially cell-free vaccines, for cancer treatment.

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Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells

Cited by 71 publications

References 47 publications

Exosomes as Biomarkers for Female Reproductive Diseases Diagnosis and Therapy

Exosomes as Biomarkers for Female Reproductive Diseases Diagnosis and Therapy

Extracellular Vesicles in Liquid Biopsies: Potential for Disease Diagnosis

Extracellular Vesicles in Oncology: from Immune Suppression to Immunotherapy

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