Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803

Wang, Yunpeng; Chen, Lei; Zhang, Weiwen

doi:10.1186/s13068-016-0627-6

Cited by 32 publications

(23 citation statements)

References 83 publications

(140 reference statements)

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“…For complete details of protein extraction, digestion, labeling with iTRAQ reagents, LC–MS/MS analysis, and data analysis, please see the Additional file 1 : Additional methods section— iTRAQ-based proteomic analysis . Proteins displaying a 1.2-fold or more change between β-carotene producing strain YBX-01 and its parental strain YBX-B ( P < 0.05) were considered as differential expressed proteins [ 50 , 51 ].…”

Section: Methodsmentioning

confidence: 99%

Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae

Lin

Cheng

et al. 2020

Biotechnol Biofuels

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Background Product toxicity is one of the bottlenecks for microbial production of biofuels, and transporter-mediated biofuel secretion offers a promising strategy to solve this problem. As a robust microbial host for industrial-scale production of biofuels, Saccharomyces cerevisiae contains a powerful transport system to export a wide range of toxic compounds to sustain survival. The aim of this study is to improve the secretion and production of the hydrophobic product (β-carotene) by harnessing endogenous ABC transporters combined with physiological engineering in S. cerevisiae. Results Substrate inducibility is a prominent characteristic of most endogenous transporters. Through comparative proteomic analysis and transcriptional confirmation, we identified five potential ABC transporters (Pdr5p, Pdr10p, Snq2p, Yor1p, and Yol075cp) for β-carotene efflux. The accumulation of β-carotene also affects cell physiology in various aspects, including energy metabolism, mitochondrial translation, lipid metabolism, ergosterol biosynthetic process, and cell wall synthesis. Here, we adopted an inducible GAL promoter to overexpress candidate transporters and enhanced the secretion and intracellular production of β-carotene, in which Snq2p showed the best performance (a 4.04-fold and a 1.33-fold increase compared with its parental strain YBX-01, respectively). To further promote efflux capacity, two strategies of increasing ATP supply and improving membrane fluidity were following adopted. A 5.80-fold increase of β-carotene secretion and a 1.71-fold increase of the intracellular β-carotene production were consequently achieved in the engineered strain YBX-20 compared with the parental strain YBX-01. Conclusions Overall, our results showcase that engineering endogenous plasma membrane ABC transporters is a promising approach for hydrophobic product efflux in S. cerevisiae. We also highlight the importance of improving cell physiology to enhance the efficiency of ABC transporters, especially energy status and cell membrane properties.

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Section: Methodsmentioning

confidence: 99%

Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae

Lin

Cheng

et al. 2020

Biotechnol Biofuels

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“…showed that overexpressing genes putatively involved in cobalt/nickel, manganese and phosphate transports could improve 3-HP production (Wang et al, 2016a).…”

Section: Optimizing 3-hp Production Through Metabolic Engineeringmentioning

confidence: 99%

Process engineering for microbial production of 3-hydroxypropionic acid

Fouchécour

Sánchez-Castañeda

Saulou‐Bérion

et al. 2018

Biotechnology Advances

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Due to concerns about the unsustainability and predictable shortage of fossil feedstocks, research efforts are currently being made to develop new processes for production of commodities using alternative feedstocks. 3-Hydroxypropionic acid (CAS 503-66-2) was recognised by the US Department of Energy as one of the most promising value-added chemicals that can be obtained from biomass. This article aims at reviewing the various strategies implemented thus far for 3-hydroxypropionic acid bioproduction. Special attention is given here to process engineering issues. The variety of possible metabolic pathways is also described in order to highlight how process design can be guided by their understanding. The most recent advances are described here in order to draw up a panorama of microbial 3-hydroxypropionic acid production: best performances to date, remaining hurdles and foreseeable developments. Important milestones have been achieved, and process metrics are getting closer to commercial relevance. New strategies are continuously being developed that involve new microbial strains, new technologies, or new carbon sources in order to overcome the various hurdles inherent to the different microbial routes.

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“…Advances in high-throughput technologies such as proteomics, transcriptomics, and metabolomics are increasingly been used to understand how specific genes are expressed and the role they play in metabolic pathways associated with biofuel production by lignocellulose degrading microorganisms [ 230 , 231 ]. Computational tools are often used to obtain a mechanistic understanding of the information derived from these advanced technologies.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges

Adegboye

Ojuederie

Talia

et al. 2021

Biotechnol Biofuels

144

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The issues of global warming, coupled with fossil fuel depletion, have undoubtedly led to renewed interest in other sources of commercial fuels. The search for renewable fuels has motivated research into the biological degradation of lignocellulosic biomass feedstock to produce biofuels such as bioethanol, biodiesel, and biohydrogen. The model strain for biofuel production needs the capability to utilize a high amount of substrate, transportation of sugar through fast and deregulated pathways, ability to tolerate inhibitory compounds and end products, and increased metabolic fluxes to produce an improved fermentation product. Engineering microbes might be a great approach to produce biofuel from lignocellulosic biomass by exploiting metabolic pathways economically. Metabolic engineering is an advanced technology for the construction of highly effective microbial cell factories and a key component for the next-generation bioeconomy. It has been extensively used to redirect the biosynthetic pathway to produce desired products in several native or engineered hosts. A wide range of novel compounds has been manufactured through engineering metabolic pathways or endogenous metabolism optimizations by metabolic engineers. This review is focused on the potential utilization of engineered strains to produce biofuel and gives prospects for improvement in metabolic engineering for new strain development using advanced technologies.

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Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803

Cited by 32 publications

References 83 publications

Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae

Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae

Process engineering for microbial production of 3-hydroxypropionic acid

Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges

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