Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)

Ji, Chenglong; Wu, Huifeng; Wei, Lei; Zhao, Jianmin; Yu, Junbao

doi:10.1016/j.aquatox.2013.07.009

Cited by 101 publications

(68 citation statements)

References 48 publications

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“…The -omics techniques (genomics, transcriptomics, proteomics, and metabolomics) can compare the whole patterns of molecules (genes, proteins, and metabolite) to present the differences between normal and stressed biological samples, and therefore, they have been extensively used to elucidate the biological effects induced by environmental stressors in organisms (Ji et al, 2013;Santos et al, 2010;Song et al, 2016aSong et al, , 2016b. Among these -omics techniques, metabolomics focuses on the whole set of metabolites (b 1000 Da) in organisms (Jones et al, 2008;Viant et al, 2003).…”

Section: H Nmrmentioning

confidence: 99%

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Zhao

et al. 2016

Marine Pollution Bulletin

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a b s t r a c t a r t i c l e i n f oMarine environment in the Laizhou Bay is potentially contaminated by metals from industrial discharges. In this study, metal concentrations in shrimps Crangon affinis indicated that two typical sites (S6283 and S5283) close to Longkou and Zhaoyuan cities along the Laizhou Bay have been contaminated by metals, including Cd, As, Cu, Ni, Co, and Mn. In particular, Cd and As were the main metal contaminants in S6283. In S5283, however, Cu was the most important metal contaminant. The metabolic responses in the shrimps indicated that the metal pollution in S6283 and S5283 induced disturbances in osmotic regulation and energy metabolism and reduced anaerobiosis, lipid metabolism, and muscle movement. However, alteration in the levels of dimethylglycine, dimethylamine, arginine, betaine, and glutamine indicated that the metal pollution in S5283 induced osmotic stress through different pathways compared to that in S6283. In addition, dimethylamine might be the biomarker of Cu in shrimp C. affinis.

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Section: H Nmrmentioning

confidence: 99%

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Zhao

et al. 2016

Marine Pollution Bulletin

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“…The total spectral area of the remaining bins was normalized to unity to facilitate the comparison between the spectra. All the NMR spectra were generalized log transformed (glog) with a transformation parameter λ = 2.0 × 10 −9 to stabilize the variance across the spectral bins and to increase the weightings of the less intense peaks (Ji et al 2013). As described in details in our previous work, data were mean-centered before multivariate analysis (Ji et al 2013).…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

“…Spectral pre-processing and multivariate analysis All one dimensional 1 H NMR spectra were converted to a data matrix using the custom-written ProMetab software in Matlab version 7.0 (The MathsWorks, Natick, MA) (Ji et al 2013). Each spectrum was segmented into bins with a width of 0.005 ppm between 0.2 and 10.0 ppm.…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

“…Metabolite extracts of mussel samples were analyzed on a Bruker AV 500 NMR spectrometer performed at 500.18 MHz (at 25°C) as described previously (Ji et al 2013). All 1 H NMR spectra were phased, baseline-corrected, and calibrated (TSP at 0.0 ppm) manually using TopSpin (version 2.1, Bruker).…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

“…Polar metabolites in these mussel samples were extracted by the modified extraction protocol as described previously (Ji et al 2013). Briefly, each mussel sample (ca.…”

Section: Metabolite Extractionmentioning

confidence: 99%

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Differential metabolic responses in three life stages of mussels Mytilus galloprovincialis exposed to cadmium

et al. 2016

Ecotoxicology

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Cadmium (Cd) is one of the most important metal contaminants in the Bohai Sea. In this work, NMR-based metabolomics was used to investigate the toxicological effects of Cd at an environmentally relevant concentration (50 µg L −1 ) in three different life stages (D-shape larval, juvenile and adult) of mussels Mytilus galloprovincialis. Results indicated that the D-shape larval mussel was the most sensitive life stage to Cd. The significantly different metabolic profiles meant that Cd induced differential toxicological effects in three life stages of mussels. Basically, Cd caused osmotic stress in all the three life stages via different metabolic pathways. Cd exposure reduced the anaerobiosis in D-shape larval mussels and disturbed lipid metabolism in juvenile mussels, respectively. Compared with the D-shape larval and juvenile mussels, the adult mussels reduced energy consumption to deal with Cd stress.

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Population Genetics, Genomics and Selective Breeding

2021

Marine Mussels

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Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)

Cited by 101 publications

References 48 publications

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Differential metabolic responses in three life stages of mussels Mytilus galloprovincialis exposed to cadmium

Population Genetics, Genomics and Selective Breeding

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